A Polyphasic Reassessment of the Genus , Reclassification of (Nakamura 1984) as comb. nov. and of (Montefusco et al. 1993) as comb. nov., and Emended Descriptions of and of Free

Abstract

Seventy-seven strains representing 10 species in the 16S rRNA group and 3 other species that exhibit phenetic relatedness to members of this group, and , were characterized genotypically and phenotypically by performing an amplified ribosomal DNA restriction analysis, a randomly amplified polymorphic DNA analysis, a fatty acid methyl ester analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, pyrolysis mass spectrometry, and API and other routine phenotypic tests. These analyses revealed distinct clusters representing subsp. larvae. subsp. , and , which confirmed the distinctness of these species, but appreciable within-species heterogeneity was observed in , and P. validus. The type strain of did not cluster with other strains of this species, and in several analyses a relationship between strains of and was observed. As the analyses showed that and are closely related to the genus , these species are reclassified as members of this genus.

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1996-10-01
2024-03-28
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