1887

Abstract

16S rRNA gene sequences of the type strains of 11 species belonging to the and groups were determined. On the basis of the results of gene sequence analyses, these species were separated into two clusters. The cluster included 10 species, namely, , and and belonged to the cluster. Moreover, the two clusters were phylogenetically distinct from other , and species. On the basis of our data, we propose reclassification of the cluster as gen. nov. and reclassification of the cluster as gen. nov. By using 16S rRNA gene sequence alignments, two specific PCR amplification primers were designed for differentiating the two new genera from each other and from other aerobic, endospore-forming organisms.

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1996-10-01
2024-03-29
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References

  1. Abe M., Kimoto M. 1984; Distribution of two types of regular-array particles in cell wall of Bacillus aneurinolyticus (Kimura et Aoyama). Microbiol. Immunol 28:841–846
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett. Appl. Microbiol 13:202–206
    [Google Scholar]
  3. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
    [Google Scholar]
  4. Avgustin G., Wright F., Flint H. J. 1994; Genetic diversity and phylogenetic relationships among strains of Prevotella (Bacteroides) ruminicola from the rumen. Int. J. Syst. Bacteriol 44:246–255
    [Google Scholar]
  5. Claus D., Berkeley R. C. W. 1986 Genus Bacillus Cohn 1872. 1105–1140 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  6. Fahmy F., Flossdorf J., Claus D. 1985; The DNA base composition of the type strains of the genus Bacillus. Syst. Appl. Microbiol 6:60–65
    [Google Scholar]
  7. Farrow J. A E., Wallbanks S., Collins M. D. 1992; Phylogenetic analysis of the genera Pianococcus, Marinococcus, and Sporosarcina and their relationship to members of the genus Bacillus. FEMS Microbiol. Lett 93:167–172
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  9. Forsman M., Sandstrom G., Sjostedt A. 1994; Analysis of 16S ribosomal DNA sequences of Francisella strains and utilization for determination of the phylogeny of the genus and for identification of strains by PCR. Int. J. Syst. Bacteriol 44:38–46
    [Google Scholar]
  10. Fox G. E., Wisotzkey J., Jurtshuk P. Jr. 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol 42:166–170
    [Google Scholar]
  11. Heyndrickx M., Vandemeulebroecke K., Hoste B., Janssen P., Kersters K., De Vos P., Logan N. A., Ali N., Berkeley R. C. W. 1996; Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1984) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1966) Ash et al. 1994, as a subspecies of P. larvae, with emended description of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens. Int. J. Syst. Bacteriol 46:270–279
    [Google Scholar]
  12. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., Hoste B., Kersters K., De Vos P., Logan N. A., Aziz A. M., Ali N., Berkeley R. C. W. 1995; Paenibacillus (formerly Bacillus) gordonae (Pichinoty et al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus. Int. J. Syst. Bacteriol 45:661–668
    [Google Scholar]
  13. Hiraishi H., Kaneko M. 1994; Use of polymerase chain reaction-amplified 16S rRNA gene sequences to identify pink-pigmented bacteria found in a potable water treatment system. Bull. Jpn. Soc. Microb. Ecol 9:55–65
    [Google Scholar]
  14. Jagoueix S., Bove J.-M., Gamier M. 1994; The phloem-limited bacterium of greening disease of citrus is a member of the a subdivision of the Proteobacteria. Int. J. Syst. Bacteriol 44:379–386
    [Google Scholar]
  15. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol 16:111–120
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  17. Manachini P. L., Fortina M. G., Parini C., Craveri R. 1985; Bacillus thermoruber sp. nov., nom. rev., a red-pigmented thermophilic bacterium. Int. J. Syst. Bacteriol 35:493–496
    [Google Scholar]
  18. Nakamura L. K. 1991; Bacillus brevis Migula 1900 taxonomy: reassociation and base composition of DNA. Int. J. Syst. Bacteriol 41:510–515
    [Google Scholar]
  19. Nakamura L. K. 1993; DNA relatedness of Bacillus brevis Migula 1900 strains and proposal of Bacillus agri sp. nov., nom. rev., and Bacillus centrosporus sp. nov., nom. rev. Int. J. Syst. Bacteriol 43:20–25
    [Google Scholar]
  20. Nielsen P., Rainey F. A., Outtrup F. A., Priest F. G., Fritze D. 1994; Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. FEMS Microbiol. Lett 117:61–66
    [Google Scholar]
  21. Niimura Y., Koh E., Yanagida F., Suzuki K., Komagata K., Kozaki M. 1990; Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic sporeforming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. Int. J. Syst. Bacteriol 40:297–301
    [Google Scholar]
  22. Priest F. G. 1993 Systematics and ecology of Bacillus. 3–16 Sonenshein A. L., Hoch J., Losick R.ed Bacillus subtilis and other grampositive bacteria: biochemistry, physiology, and molecular genetics American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  23. Rainey F. A., Fritze D., Stackebrandt E. 1994; The phylogenetic diversity of thermophilic members of the genus Bacillus as revealed by 16S rDNA analysis. FEMS Microbiol. Lett 115:205–212
    [Google Scholar]
  24. Rossler D., Ludwig W., Schleifer K. H., Lin C., McGill T. J., Wisotzkey J. D., Jurtshuk P. Jr., Fox G. E. 1991; Phylogenetic diversity in the genus Bacillus as seen by 16S rRNA sequencing studies. Syst. Appl. Microbiol 14:266–269
    [Google Scholar]
  25. Saitou N., Nei M. 1987; A neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  27. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  28. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1995; Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., and Bacillus borstelensis sp. nov., nom. rev. Int. J. Syst. Bacteriol 45:93–100
    [Google Scholar]
  29. Shida O., Takagi H., Kadowaki K., Udaka S., Komagata K. 1994; Bacillus galactophilus is a later subjective synonym of Bacillus agri. Int. J. Syst. Bacteriol 44:172–173
    [Google Scholar]
  30. Shida O., Takagi H., Kadowaki K., Yano H., Abe M., Udaka S., Komagata K. 1994; Bacillus aneurinolyticus sp. nov., nom. rev. Int. J. Syst. Bacteriol 44:143–150
    [Google Scholar]
  31. Shida O., Takagi H., Kadowaki K., Yano H., Komagata K. 1996; Differentiation of species in the Bacillus brevis group and the Bacillus aneurinolyticus group based on the electrophoretic whole-cell protein pattern. Antonie Leeuwenhoek 70:31–39
    [Google Scholar]
  32. Spanka R., Fritze D. 1993; Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. Int. J. Syst. Bacteriol 43:150–156
    [Google Scholar]
  33. Suzuki T., Yamasato K. 1994; Phylogeny of spore-forming lactic acid bacteria based on 16S rRNA gene sequences. FEMS Microbiol. Lett 115:13–18
    [Google Scholar]
  34. Takagi H., Shida O., Kadowaki K., Komagata K., Udaka S. 1993; Characterization of Bacillus brevis, with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov. Int. J. Syst. Bacteriol 43:221–231
    [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  36. Towbin H., Staehlin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354
    [Google Scholar]
  37. Udaka S. 1976; Screening for protein-producing bacteria. Agric. Biol. Chern 40:523–528
    [Google Scholar]
  38. Wisotzkey J. D., Jurtshuk P. Jr., Fox G. E., Deinhard G., Poralla K. 1992; Comparative sequence analysis on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int. J. Syst. Bacteriol 42:263–269
    [Google Scholar]
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