The phylogenetic relationships of 20 relapsing fever (RF) spp. were estimated on the basis of the sequences of genes. Complete sequences were aligned and compared with previously published sequences, and the similarity values were found to be 97.7 to 99.9%. Phylogenetic trees were constructed by using the three neighbor-joining, maximum-parsimony, and maximum-likelihood methods. The results of the comparative phylogenetic analysis divided the RF spp. into three major clusters. One cluster included , and Another cluster comprised two main branches with , and on one side and , and on the other side. constituted the third cluster. The phylogenetic position of was more uncertain. These results suggested that the taxonomy of these spirochetes should be revised. To overcome the problems of culturing the spirochetes, RF primers were defined. Following PCR amplification of the gene, restriction length fragment polymorphism could be used to distinguish between RF strains.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error