1887

Abstract

The phylogenetic relationships of 20 relapsing fever (RF) spp. were estimated on the basis of the sequences of genes. Complete sequences were aligned and compared with previously published sequences, and the similarity values were found to be 97.7 to 99.9%. Phylogenetic trees were constructed by using the three neighbor-joining, maximum-parsimony, and maximum-likelihood methods. The results of the comparative phylogenetic analysis divided the RF spp. into three major clusters. One cluster included , and Another cluster comprised two main branches with , and on one side and , and on the other side. constituted the third cluster. The phylogenetic position of was more uncertain. These results suggested that the taxonomy of these spirochetes should be revised. To overcome the problems of culturing the spirochetes, RF primers were defined. Following PCR amplification of the gene, restriction length fragment polymorphism could be used to distinguish between RF strains.

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1996-10-01
2022-08-13
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