1887

Abstract

Pulsed-field gel electrophoresis was used to characterize the genomes of 15 strains. An analysis of chromosomal macrorestriction patterns allowed us to determine the chromosome sizes, which ranged from 1,435 to 3,342 kb depending on the strain. Moreover, this analysis revealed that, even though the genus is a phylogenetically coherent taxon, there is a great deal of genomic heterogeneity within it. This heterogeneity was corroborated by performing a ribotyping analysis, which showed that the rRNA hybridization patterns of most strains are unique.

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1996-10-01
2024-04-16
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References

  1. Avissar Y. J., Beale S. I. 1990; Cloning and expression of a structural gene from Chlorobium vibrioforme that complements the hemA mutation in Escherichia coli. J. Bacteriol 172:1656–1659
    [Google Scholar]
  2. Cole S. T., Saint Girons I. 1994; Bacterial genomics. FEMS Microbiol. Rev 14:139–160
    [Google Scholar]
  3. Gibson J., Ludwig W., Stackebrandt E., Woese C. R. 1985; The phylogeny of the green photosynthetic bacteria: absence of a close relationship between Chlorobium and Chloroflexus. Syst. Appl. Microbiol 6:152–156
    [Google Scholar]
  4. Guerrero R., Montesinos E., Pedrós-Alió C., Esteve I., Mas J., van Gemerden H., Hofman P. A. G., Bakker J. F. 1985; Phototrophic sulfur bacteria in two Spanish lakes: vertical distribution and limiting factors. Limnol. Oceanogr 30:919–931
    [Google Scholar]
  5. Kjaerulff S., Diep D. B. J., Okkels S., Scheller H. V., Ormerod J. G. 1994; Highly efficient integration of foreign DNA into the genome of the green sulfur bacterium Chlorobium vibrioforme by homologous recombination. Photosynth. Res 41:277–283
    [Google Scholar]
  6. McClelland M., Jones M., Patel Y., Nelson M. 1987; Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res 15:5985–6005
    [Google Scholar]
  7. Méndez-Álvarez S., Pavón V., Esteve I., Guerrero R., Gaju N. 1994; Transformation of Chlorobium limicola by a plasmid that confers the ability to utilize thiosulfate. J. Bacteriol 176:7395–7397
    [Google Scholar]
  8. Méndez-Álvarez S., Pavón V., Esteve I., Guerrero R., Gaju N. 1995; Genomic heterogeneity in Chlorobium limicola: chromosomic and plasmidic differences among strains. FEMS Microbiol. Lett 134:279–285
    [Google Scholar]
  9. Nassar A., Bertheau Y., Dervin C., Narcy J., Lemattre M. 1994; Ribotyping of Erwinia chrysanthemi strains in relation to their pathogenic and geographic distribution. Appl. Environ. Microbiol 60:3781–3789
    [Google Scholar]
  10. Naterstad K., Kolsto A., Sirevag R. 1995; Physical map of the genome of the green phototrophic bacterium Chlorobium tepidum. J. Bacteriol 177:5480–5484
    [Google Scholar]
  11. Pfennig N. 1989 Green sulfur bacteria. 1682–1709 Staley J. T., Bryant M. P., Pfennig N., Holt J. G.ed Bergey’s manual of systematic bacteriology 3 Williams and Wilkins; Baltimore:
    [Google Scholar]
  12. Pfennig N., Trüper H. G. 1992 The family Chlorobiaceae,. 3583–3592 Starr M. P., Stolp H., Triiper H. G., Schlegel H. G.ed The prokaryotes Springer-Verlag; Berlin:
    [Google Scholar]
  13. Pibernat I. 1996 Ph.D. thesis Universitat de Girona; Girona, Spain:
  14. Rodrigo A. G., Borges K. M., Bergquist P. L. 1994; Pulsed-field gel electrophoresis of genomic digests of Thermus strains and its implications for taxonomic and evolutionary studies. Int. J. Syst. Bacteriol 44:547–552
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  16. Sameeh S. M., Garcia M., Taylor D. E. 1992; Differentiation of the subspecies of Campylobacter fetus by genomic sizing. Int. J. Syst. Bacteriol 42:446–450
    [Google Scholar]
  17. Schwartz D. C., Cantor C. R. 1984; Separation of yeast chromosome sized DNAs by pulsed field gel gradient electrophoresis. Cell 37:67–75
    [Google Scholar]
  18. Smith C. L., Cantor C. R. 1987; Purification, specific fragmentation and separation of large DNA molecules. Methods Enzymol 155:449–467
    [Google Scholar]
  19. Smith C. L., Klco C. R., Cantor C. R. 1988 Pulsed field gel electrophoresis and the technology of large DNA molecules. 211–272 Davies K. E.ed Genome analysis A practical approach. IRL; Oxford:
    [Google Scholar]
  20. Van Gemerden H., Beeftink H. H. 1983 Ecology of phototrophic bacteria. 146–185 Ormerod J. G.ed The phototrophic bacteria Blackwell; Oxford:
    [Google Scholar]
  21. Wahlund T. M., Madigan M. T. 1995; Genetic transfer by conjugation in the thermophilic green sulfur bacterium Chlorobium tepidum. J. Bacteriol 177:2583–2588
    [Google Scholar]
  22. Wahlund T. M., Woese C. R., Castenholz R. W., Madigan M. T. 1991; A thermophilic green sulfur bacterium from New Zealand hot springs, Chlorobium tepidum sp. nov. Arch. Microbiol 156:81–90
    [Google Scholar]
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