1887

Abstract

A novel proteolytic bacterium was isolated from rumen contents of New Zealand cattle grazing fresh forage and was designated strain B316 (T = type strain). Strain B316 cells were straight to slightly curved rods (width, 0.4 to 0.6 μm; length, 1.3 to 3.0 μm) that were gram-positive and possessed a single subterminal flagellum. This isolate did not produce catalase, indole, ammonia, lipase, or lecithinase or reduce nitrate, but it did produce a curd reaction with milk. Strain B316 was proteolytic, hydrolyzing casein and fraction I leaf protein. The crude proteinase was predominantly the serine type, but some cysteine proteinase and metallo-proteinase activities were also detected. The DNA base composition of strain B316 was 28 mol% G+C. A 16S ribosomal DNA sequence analysis of strain B316 indicated that it was most closely related to a member of clostridial cluster XIVa, viz., , an amino acid-fermenting organism isolated from the rumen; the similarity value was 92.2%. The results of the phenotypic characterization analysis, G+C content analysis, and phylogenetic analysis of the 16S ribosomal DNA sequence set strain B316 apart from all of the members of cluster XIVa. We propose that strain B316 should be designated a new species of the genus . Strain B316 is the type strain and has been deposited in the American Type Culture Collection as strain ATCC 51982.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-3-753
1996-07-01
2024-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/3/ijs-46-3-753.html?itemId=/content/journal/ijsem/10.1099/00207713-46-3-753&mimeType=html&fmt=ahah

References

  1. Attwood G. T., Reilly K. 1995; Identification of proteolytic rumen bacteria isolated from New Zealand cattle. J. Appl. Bacteriol 79:22–29
    [Google Scholar]
  2. Brock F. M., Forsbei C. W., Buchanan-Smith J. G. 1982; Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl. Environ. Microbiol 44:561–569
    [Google Scholar]
  3. Bryant M. P. 1984 Butyrivibrio. 641–643 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Bryant M. P. 1984 Lachnospira. 661–662 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  5. Charney J., Tomarelli R. M. 1947; A colorimetric method for the determination of the proteolytic activity of duodenal juice. J. Biol. Chem 171:501–505
    [Google Scholar]
  6. Chen G., Russell J. B. 1988; Fermentation of peptides and amino acids by a monensin-sensitive ruminal Peptostreptococcus. Appl. Environ. Microbiol 54:2742–2749
    [Google Scholar]
  7. Chen G., Russell J. B. 1989; More monensin-sensitive, ammoniaproducing bacteria from the rumen. AppL Environ. Microbiol 55:1052–1057
    [Google Scholar]
  8. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium-, proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol 44:812–826
    [Google Scholar]
  9. Doetsch R. N. 1981 Determinative methods of light microscopy. 21–33 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  10. Forster R. Personal communication
  11. Hazlewood G. P., Edwards R. 1981; Proteolytic activities of a rumen bacterium, Bacteroides ruminicola R8/4. J. Gen. Microbiol 125:11–15
    [Google Scholar]
  12. Holdeman L. V., Moore W. E. C. 1972 Anaerobe laboratory manual Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism 3 Academic Press, Inc.; New York:
    [Google Scholar]
  14. Leedk J. A., Hespell R. B. 1980; Differential carbohydrate media and anaerobic replica plating technique in delineating carbohydrate utilizing subgroups in rumen bacterial populations. Appl. Environ. Microbiol 39:709–719
    [Google Scholar]
  15. MacRae J. C., Ulyatt M. J. 1974; Quantitative digestion of fresh herbage by sheep. IL The sites of digestion of some nitrogenous constituents. J. Agric. Sci 82:309–319
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular cloning: a laboratory manual Cold Spring Harbor Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  17. Moore W. E. C., Holdeman-Moore L. V. 1986 Eubacterium. 1353–1373 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  18. Nugent J. H. A., Mangan J. L. 1978; Rumen proteolysis of fraction 1 leaf protein, casein and bovine serum albumin. Proc. Nutr. Soc 37:48
    [Google Scholar]
  19. Nugent J. H. A., Mangan J. L. 1981; Characteristics of the rumen proteolysis of fraction I (18S) leaf protein from lucerne (Medicago sativa L.). Br. J. Nutr 46:39–59
    [Google Scholar]
  20. Rees G. N., Grassia G. S., Sheehy A. J., Dwivedi P. P., Patel B. K. C. 1995; Desulfacinum infemum gen. nov., sp. nov., a thermophilic sulfatereducing bacterium from a petroleum reservoir. Int. J. Syst. Bacteriol 45:85–89
    [Google Scholar]
  21. Russell J. B., Strobel H. J., Chen G. 1988; The enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production. Appl. Environ. Microbiol 54:872–877
    [Google Scholar]
  22. Saito H., Miura K.-I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619–629
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  25. Spiers A. G., Hopcroft D. H. 1992; Some electron microscope observations of conidium ontogeny of Sphaceioma tnurrayae on Salix. N. Z. J. Bot 30:353–358
    [Google Scholar]
  26. Twining S. S. 1984; Fluorescein isothiocyanate-labelled casein assay for proteolytic enzymes. Anal. Biochem 143:30–34
    [Google Scholar]
  27. Wallace R. J., Joblin K. N. 1985; Proteolytic activity of a rumen anaerobic fungus. FEMS Microbiol. Lett 29:19–25
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-3-753
Loading
/content/journal/ijsem/10.1099/00207713-46-3-753
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error