1887

Abstract

A bacterial strain isolated from a fluoranthene-polluted soil was characterized with respect to its metabolic and chemotaxonomic properties, and its phylogenetic position was determined. This bacterium exhibits all of the genus-specific properties of the genus and clusters phylogenetically with the group of fast-growing mycobacteria. On the basis of its unique fatty acid pattern, the distinctness of its physiological properties and the uniqueness of the primary structure of its 16S ribosomal DNA, we propose that the new isolate should be assigned to a new species, . This novel species is phylogenetically closely related to and . The type strain of is strain EMI2 (= DSM 44183).

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1996-07-01
2024-07-19
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References

  1. Bonicke R. 1961; Die Bedeutung der Acylamidasen fur die Identifizierung und Differenzierung der verschiedenen Arten der Gattung Mycobacterium. Jahresber. Borstel 5:7–87
    [Google Scholar]
  2. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  3. Deutsche Sammlung von Mikroorganismen 1993 DSM catalogue of strains. , 5th. Deutsche Sammlung von Mikroorganismen; Braunschweig, Germany:
    [Google Scholar]
  4. Felsenstein J. 1993 PHYLIP (phylogeny inference package), version 3.5c University of Washington; Seattle:
    [Google Scholar]
  5. Finnerty W. R. 1992; The biology and genetics of the genus Rhodococcus. Annu. Rev. Microbiol 46:193–218
    [Google Scholar]
  6. Haggeblom M. M., Nohynek L. J., Palleroni N. J., Kronquist K., Nurmiaho-Lassila E.-L., Salkinoja-Salonen M. S., Klatte S., Kroppenstedt R. 1994; Transfer of polychlorophenol-degrading Rhodococcus chlorophenolicus (Apajalahti et al. 1986) to the genus Mycobacterium as Mycobacterium chlorophenolicum comb. nov. Int. J. Sy st. Bacteriol 44:485–493
    [Google Scholar]
  7. Hartmans S., deBont J. A. M. 1992; Aerobic vinyl chloride metabolism in Mycobacterium aurum LI. AppL Environ. Microbiol 58:1220–1226
    [Google Scholar]
  8. Heitkamp M. A., Cerniglia C. E. 1988; Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field. AppL Environ. Microbiol 54:1612–1614
    [Google Scholar]
  9. Hinrikson H. P., Pfyffer G. E. 1994; Mycobacterial my colic acids. Med. Microbiol. Lett 3:49–57
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  11. Kampfer P., Dott W., Kroppenstedt R. M. 1990; Numerical classification and identification of some nocardioform bacteria. J. Gen. AppL Microbiol 35:309–331
    [Google Scholar]
  12. Kampfer P., Kroppenstedt R. M. 1994; Enzymatic profiles of Mycobacterium spp. and their value for species differentiation. Microbiology 140:209–214
    [Google Scholar]
  13. Kelley I., Freeman J. P., Evans F. E., Cerniglia C. E. 1993; Identification of metabolites from the degradation of fluoranthene by Mycobacterium sp. strain PYR-1. AppL Environ. Microbiol 59:800–806
    [Google Scholar]
  14. Kirchof H., Maas C., Runge M., Franz B., Schmidt R., Quentmeier H., Miihlradt P. F. 1992; Tetrazolium-[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction by mycoplasmas. Int. J. Syst. Bacteriol 42:506–508
    [Google Scholar]
  15. Klatte S., Kroppenstedt R. M., Rainey F. A. 1994; Rhodococcus opacus sp. nov., an unusual nutritionally versatile Rhodococcus species. Syst. AppL Microbiol 17:335–360
    [Google Scholar]
  16. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. Soc. AppL Bacteriol. Tech. Ser 20:173–199
    [Google Scholar]
  17. Kroppenstedt R. M., Korn-Wendisch F., Fowler V. F., Stackebrandt E. 1981; Biochemical and molecular genetic evidence for transfer of Actinoplanes armeniacus into the family Streptomycetaceae. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 2:254–262
    [Google Scholar]
  18. Kubica G. P., Pool G. L. 1960; Studies on the catalase activity of acid fast bacilli. I. An attempt to subgroup these organisms on the basis of their catalase activities at different temperatures and pH. Am. Rev. Respir. Dis 81:387–391
    [Google Scholar]
  19. Kuykendall L. D., Roy M. A., O’Neill J. J., Devine T- E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int. J. Syst. Bacteriol 38:358–361
    [Google Scholar]
  20. Lefford M. J. 1980 Mycobacterial culture collection. National Institutes of Health Publication 80–289 U.S. Department of Health and Human Services; Washington, D.C:
    [Google Scholar]
  21. Levy-Frebault V. V., Portaels F. 1992; Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int. J. Syst. Bacteriol 42:315–323
    [Google Scholar]
  22. Luquin M., Ausina V., Lopez Calahorra F., Belda F., Garcia Barcelo M., Celma C., Prats G. 1991; Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J. Clin. Microbiol 29:120–130
    [Google Scholar]
  23. Maidak B. L., Larsen N., McCaughey M. A., Overbeek R., Olsen G. J., Fogel K., Blandy J., Woese C. R. 1994; The Ribosomal Database Project. Nucleic Acids Res 22:3485–3487
    [Google Scholar]
  24. Malachowsky K. J., Phelps T. J., Teboli A. B., Minnikin D. E., White D. C. 1994; Aerobic mineralization of trichloroethylene, vinylchloride, and aromatic compounds by Rhodococcus species. AppL Environ. Microbiol 60:542–548
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol 39:159–167
    [Google Scholar]
  26. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J. Clin. Microbiol 16:584–586
    [Google Scholar]
  27. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J. Chromatogr 188:221–233
    [Google Scholar]
  28. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J. Microbiol. Methods 2:233–24
    [Google Scholar]
  29. Pitulle C., Dorsch M., Kazda J., Wolters J., Stackebrandt E. 1992; Phylogeny of rapidly growing members of the genus Mycobacterium. Int. J. Syst. Bacteriol 42:337–343
    [Google Scholar]
  30. Rafii F., Butler W. R., Cerniglia C. E. 1992; Differentiation of rapidly growing, scotochromogenic, polycyclic-aromatic-hydrocarbon metabolizing strains of Mycobacterium sp. from other known Mycobacterium species. Arch. Microbiol 157:512–520
    [Google Scholar]
  31. Rainey F. A., Dorsch M., Morgan H. W., Stackebrandt E. 1992; 16S rDNA analysis of Spirochaeta thermophila: its phylogenetic position and implications for the systematics of the order Spirochaetales. Syst. Appl. Microbiol 15:197–202
    [Google Scholar]
  32. Rainey F. A., Klatte S., Kroppenstedt R. M., Stackebrandt E. 1995; Dietzia, a new genus including Dietzia marls comb. nov., formerly Rhodococcus marls. Int. J. Syst. Bacteriol 45:32–36
    [Google Scholar]
  33. Rainey F. A., Lang E. Unpublished data
  34. Silcox V. A., Good R. C., Floyd M. M. 1981; Identification of clinically significant Mycobacterium fortuitum complex isolates. J. Clin. Microbiol 14:686–691
    [Google Scholar]
  35. Stackebrandt E., Smida J. 1988 The phylogeny of the genus Mycobacterium as determined by 16S rRNA sequences, and development of DNA probes. 244–250 Okami Y., Beppu T. T., Ogawara H.ed Biology of actinomycetes Japan Scientific Press; Tokyo:
    [Google Scholar]
  36. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl. Microbiol 28:226–231
    [Google Scholar]
  37. Tiem B. A., Boldrien B., Fritsche C. 1993; Degradation of phenanthrene, fluorene fluoranthene, and pyrene by a Mycobacterium species. Appl. Environ. Microbiol 59:1927–1930
    [Google Scholar]
  38. Tsukamura M., van der Meulen H., Grabow W. O. K. 1983; Numerical taxonomy of rapidly growing, scotochromogenic mycobacteria of the Mycobacterium parafortuitum complex: Mycobacterium austroafncanum sp. nov. and Mycobacterium diemhoferi sp. nov., nom. rev. Int. J. Syst. Bacteriol 33:460–469
    [Google Scholar]
  39. Wang R. F., Cao W. W., Cerniglia C. E. 1995; Phylogenetic analysis of polycyclic aromatic hydrocarbon degrading mycobacteria by 16S rRNA sequencing. FEMS Microbiol. Lett 130:75–80
    [Google Scholar]
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