1887

Abstract

During a survey of bacterial agents that cause subclinical mastitis in water buffalos, we isolated several strains of gram-positive cocci that appeared to be enterococci except that they grew very slowly at 45°C and grew slowly in broth containing 6.5% NaCl. On the basis of the results of conventional physiologic tests, these strains were identified as However, none of the strains reacted with the AccuProbe genetic probe. The whole-cell protein profiles of these organisms were compared with the profiles of and reference strains. Apart from minor quantitative differences, the mastitis isolates had indistinguishable protein profiles that were similar to the profiles of the and type strains. The results of DNA relatedness studies performed by using the hydroxy-apatite method at 55 and 70°C indicated that all of the mastitis isolates were related to the type strain of at the species level, despite the fact that they exhibited several uncommon phenotypic characteristics (growth at 45°C, growth in broth containing 6.5% NaCl, and failure to produce acid from mannitol and sucrose). The high levels of DNA relatedness between strains of and demonstrated that these taxa are members of a single species. Since is a senior synonym of should be retained as the species name and strain ATCC 43921 should remain the type strain of this species.

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1996-07-01
2024-04-17
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References

  1. Aguirre M., Collins M. D. 1993; Lactic acid bacteria and human clinical infection. J. Appl. Bacteriol 75:95–107
    [Google Scholar]
  2. Brenner D. J., McWhorter A. C., Leete Knutson J. K., Steigerwalt A. G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. J. Clin. Microbiol 15:1133–1140
    [Google Scholar]
  3. Collins M. D., Farrow J. A. E., Phillips B. A., Kandler O. 1983; Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J. Gen. Microbiol 129:3427–3431
    [Google Scholar]
  4. Domenech A., Prieta J., Fernandez-Garayzabal J. F., Collins M. D., Jones D., Dominguez L. 1993; Phenotypic and phylogenetic evidence for a close relationship between Lactococcusgarvieae and Enterococcus seriolicida. Microbiol. Sem 9:63–68
    [Google Scholar]
  5. Elliot J. A., Collins M. D., Pigott N. E., Facklam R. R. 1991; Differentiation of Lactococcus lactis and Lactococcus garvieae from humans by comparison of whole-cell protein patterns. J. Clin. Microbiol 29:2731–2734
    [Google Scholar]
  6. Facklam R. R., Collins M. D. 1989; Identification of Enterococcus species isolated from human infections by a conventional test scheme. J. Clin. Microbiol 27:731–734
    [Google Scholar]
  7. Facklam R. R., Elliot J. A. 1995; Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin. Microbiol. Rev 8:479–495
    [Google Scholar]
  8. Facklam R. R., Sahm D. F. 1995 Enterococcus. 308–314 Murray P. R., Baron E. J, Pfaller M. A, Tenover F. C., Yolken R. H.ed Manual of clinical microbiology, 6th. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  9. Kusuda R., Kawai K., Salati F., Banner C. R., Fryer J. L. 1991; Enterococcus seriolicida sp. nov., a fish pathogen. Int. J. Syst. Bacteriol 41:406–409
    [Google Scholar]
  10. Merquior V. L. C., Peralta J. M., Facklam R. R., Teixeira L. M. 1994; Analysis of electrophoretic whole-cell protein profiles as a tool for characterization of Enterococcus species. Curr. Microbiol 28:149–153
    [Google Scholar]
  11. Ruoff K. L. 1995 Leuconostoc, Pediococcus, Stomatococcus, and miscellaneous gram-positive cocci that grow aerobically. 315–323 Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H.ed Manual of clinical microbiology, 6th. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  12. Schleifer K. H., Klaus J., Dvorak C., Kilpper-Balz R., Collins M. D., Fischer W. 1985; Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov. Syst. Appl. Microbiol 6:183–195
    [Google Scholar]
  13. Teixeira L. M., Facklam R. R., Steigerwalt A. G., Pigott N. E., Merquior V. L. C., Brenner D. J. 1995; Correlation between phenotypic characteristics and DNA relatedness within Enterococcus faecium strains. J. Clin. Microbiol 33:1520–1523
    [Google Scholar]
  14. Vianni M. C. E. et al. Unpublished data
  15. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol 37:463–464
    [Google Scholar]
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