*Corresponding author. Mailing address: Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Cresent Singapore 119260. Phone: 65 7783207. Fax: 65 7791117. Electonic mail address: mebwangy@leonis.nus.sg.
The 16S rRNA gene sequences of 7 Microbispora strains, 14 Microtetraspora species, 9 Streptosporangium species, and 12 Actinomadura species were determined. A phylogenetic analysis showed that Microtetraspora fusca, Microtetraspora glauca, and Microtetraspora niveoalba formed a coherent cluster with the members of the genus Microbispora. This cluster is distantly related to two other clusters, one of which consists of the Microtetraspora species transferred from the former Actinomadura pusilla group and one of which consists of members of the genus Streptosporangium. Our results show that it is necessary to review the taxonomic definitions of the genera Microtetraspora and Microbispora, which have been separated by a single morphological characteristic, the number of spores on spore chains.
BrosiusJ., PalmerJ. J., KennedyJ. P., NollerH. F.1978; Complete nucleotide sequence of a 16S ribosomal gene from Escherichia coll. Proc. Natl. Acad. Sci. USA 75:4801–1805
GoodfellowM.1989 Maduromycetes. 2509–2551WilliamsS. T., SharpeM. E., HoltJ. G.edBergy’s manual of systematic bacteriology4 The Williams & Wilkins Co.; Baltimore:
GoodfellowM.1992 The family Streptosporangiaceae. 1116–1138BalowsA., TriiperH. G., DworkinM., HarderW., SchieiferK.-H.edThe prokaryotes, 2nd. Springer-Verlag; New York:
GoodfellowM., StantonL. J., SimpsonK. E., MinnikinD. E.1990; Numerical and chemical classification of Actinoplanes and some related actinomycetes. J. Gen. Microbiol 136:19–34
KroppenstedtR., StackebrandtE., GoodfellowM.1990; Taxonomic revision of the actinomycete genera Actinomadura and Microtetraspora. Syst. Appl, Microbiol 13:148–160
KroppenstedtR. M., GoodfellowM.1992 The family Thermomonosporaceae. 1085–1114BalowsA, TriiperH. G., DworkinM., HarderW., SchieiferK.-H.edThe prokaryotes, 2nd. Springer-Verlag; New York:
MiyadohS., AmanoS., TohyamaH., ShomuraT.1990; A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea. J. Gen. MicrobioL 136:1905–1913
MiyadobS., AnzaiH., AmanoS., ShomuraT.1989; Actinomadura malachitica and Microtetraspora viridis are synonyms and should be transferred as Actinomadura viridis comb. nov. Int. J. Syst. Bacteriol 39:152–158
NonomuraH., OharaY.1971; Distribution of actinomycetes in soil. VIII. Green-spore group of Microtetraspora, its preferential isolation and taxonomic characteristics. J. Ferment. Technol 49:1–7
NonomuraH., OharaY.1971; Distribution of actinomycetes in soil. IX. New species of genera Microbispora and Microtetraspora, and their isolation method. J. Ferment TechnoL 49:887–894
OchiK., HaraguchiK., MiyadohS.1993; A taxonomic review of the genus Microbispora by analysis of ribosomal protein AT-L30. Int. J. Syst. Bacteriol 43:58–62
OchiK., MiyadohS.1992; Polyacrylamide gel electrophoresis analysis of ribosomal protein AT-L30 from an actinomycete genus, Streptosporangium. Int. J. Syst. Bacteriol 42:151–155
OchiK., MiyadohS., TamuraT.1991; Polyacrylamide gel electrophoresis analysis of ribosomal protein AT-L30 as a novel approach to actinomycete taxonomy: application to the genera Actinomadura and Microtetraspora. Int. J. Syst. Bacteriol 41:234–239
RaineyF. A., BurgardtJ., KroppenstedtR. M., KlatteS., StackebrandtE.1995; Phylogenetic analysis of the genera Rhodococcus and Nocardia and evidence for the evolutionary origin of the genus Nocardia from within the radiation of Rhodococcus species. Microbiology 141:523–528
StackebrandtE., GoebelB. M.1994; Taxonomic note: a place for DNA-DNA association and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol 44:846–849
StackebrandtE., KroppenstendtR. M., JahnkeK.-D., KemmerlingC., GurtlerH.1994; Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb, nov., Kutzneria Kofuensis comb, nov., and Kutzneria albida comb, nov., respectively, and emendation of the genus Streptosporangium. Int. J. Syst. Bacteriol 44:265–269
WoeseC. R., KandlerO., WheelisM. L.1990; Towards a natural system of organisms: proposal for the domains Archaea, Bacteria and Eucarya. Proc. NatL Acad. Sci. USA 87:4576–4579