, and (M.-C. Curk, J.-C. Hubert, and F. Bringel, Int. J. Syst. Bacteriol. 46:595–598, 1996) can hardly be distinguished on the basis of their phenotypes. Unlike and ferments glycerol and xylose but not melezitose. We identified two strains (CNRZ 1538 and CNRZ 1544) which ferment glycerol and melezitose but not xylose. α-Methyl-D-mannoside was fermented by 66% of the strains tested but not by strains. In this paper we describe a simple method to identify , and . This method is based on nonradioactive Southern-type hybridization between I DNA digests of the lactobacilli tested and a DNA probe ( genes from strain CCM 1904). A total of 68 lactobacilli were classified into five groups on the basis of the bands detected. Two groups contained strains; one of these groups contained 31 strains, including the type strain, and was characterized by bands at 7, 4, and 1 kb, and the other group contained strain LP 85-2 and was characterized by bands at 5 and 1.1 kb. Only one band (a band at around 7 kb) was detected in the strains belonging to the group, and two bands (at 4 and 1 kb) were found in the strains belonging to the group. No hybridization was detected in the last group, which contained , and strains.


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