1887

Abstract

A gram-negative, anaerobic, non-spore-forming bacterium which is a curved rod and motile by means of a single polar or subpolar flagellum was isolated from the rumen of a cow on pasture. The bacterium fermented a range of carbohydrates. Glucose was fermented to formate, butyrate, and lactate. The composition of cellular fatty acids was determined. The DNA base composition was 40 to 41 mol% G+C. The complete 16S rRNA sequence (EMBL accession number, X95893) was obtained, and the phylogenetic relationships were determined. The most closely related taxa were , and . The name proposed for this bacterium is gen. nov., the type strain is A12-1 (DSM 9787).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-2-559
1996-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/2/ijs-46-2-559.html?itemId=/content/journal/ijsem/10.1099/00207713-46-2-559&mimeType=html&fmt=ahah

References

  1. Bryant M. P. 1984 Genus Butyrivibrio Bryant and Small 1956, 18, emend. Moore, Johnson and Holdeman 1976, 241AL. 641–643 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  2. Biyant M. P., Small N. 1956; The anaerobic monotrichous butyric acidproducing curved rod-shaped bacteria of the rumen. J. Bacteriol 72:16–21
    [Google Scholar]
  3. Caldwell D. R., Bryant M. P. 1966; Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. AppL Microbiol 14:794–801
    [Google Scholar]
  4. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridiumproposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol 44:812–826
    [Google Scholar]
  5. Cornick N. A., Jensen N. S., Stahl D. A., Hartman P. A., Allison M. J. 1994; Lachnospira pectinoschiza sp. nov., an anaerobic pectinophile from the pig intestine. Int. J. Syst. Bacteriol 44:87–93
    [Google Scholar]
  6. Hespell R. B. 1992 The genera Butyrivibrio, Lachnospira, and Roseburia,. 2022–2033 Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. 2 Springer-Verlag; New York:
    [Google Scholar]
  7. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobe laboratory manual. , 4th. Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  8. Holdeman L. V., Moore W. E. C. 1974; New genus, Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human feces. Int. J. Syst. Bacteriol 24:260–277
    [Google Scholar]
  9. Hungate R. E. 1966 The rumen and its microbes Academic Press, Inc.; New York:
    [Google Scholar]
  10. Ifkovits R. W., Ragheb R. S. 1968; Cellular fatty acid composition and identification of rumen bacteria. Appl. Microbiol 16:1406–1413
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  12. Leatherwood J. M., Sharma M. P. 1972; Novel anaerobic cellulolytic bacterium. J. Bacteriol 110:751–753
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol 3:208–218
    [Google Scholar]
  14. Meier A., Kirschner P., Schroder K.-H., Wolters J., Kroppenstedt R. M., Bottger E. C. 1993; Mycobacterium intermedium sp. nov. Int. J. Syst. Bacteriol 43:204–209
    [Google Scholar]
  15. Meyer S. A., Schleifer K. H. 1975; Rapid procedure for the approximate determination of the deoxyribonucleic acid base composition of micrococci, staphylococci, and other bacteria. Int. J. Syst. Bacteriol 25:383–385
    [Google Scholar]
  16. Moore L. V. H., Bourne D. M., Moore W. E. C. 1994; Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int. J. Syst. Bacteriol 44:338–347
    [Google Scholar]
  17. Moore W. E. C., Holdeman L. V. 1974; Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl. Microbiol 27:961–979
    [Google Scholar]
  18. Moore W. E. C., Holdeman Moore L. V. 1986 Genus Eubacterium Prevot 1938, 294AL. 1353–1373 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology, voL 2 Williams & Wilkins; Baltimore:
    [Google Scholar]
  19. Moore W. E. C., Johnson J. L., Holdeman L. V. 1976; Emendation of Bacteroidaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species in the genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus. Int. J. Syst. Bacteriol 26:238–252
    [Google Scholar]
  20. Nakazawa F., Hoshino E. 1994; Genetic relationships among Eubacterium species. Int. J. Syst. Bacteriol 44:787–790
    [Google Scholar]
  21. Rainey F. A., Dorsch M., Morgan H. W., Stackebrandt E. 1992; 16S rDNA analysis of Spirochaeta thermophila\ position and implications for the systematics of the order Spirochaetales. Syst. Appl. Microbiol 16:224–226
    [Google Scholar]
  22. Rainey F. A., Stackebrandt E. 1993; 16S rDNA analysis reveals phylogenetic diversity among the polysaccharolytic clostridia. FEMS Microbiol. Lett 113:125–128
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  24. Stanton T. B., Savage D. C. 1983; Roseburia cecicola gen. nov., sp. nov., a motile, obligately anaerobic bacterium from a mouse cecum. Int. J. Syst. Bacteriol 33:618–627
    [Google Scholar]
  25. Stewart C. S., Bryant M. P. 1988 The rumen bacteria. 21–75 Hobson P. N.ed The rumen microbial ecosystem Elsevier Applied Science; London:
    [Google Scholar]
  26. Ulitzur S. 1972; Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochim. Biophys. Acta 272:1–11
    [Google Scholar]
  27. Vainshtein M., Hippe H., Kroppenstedt R. M. 1992; Cellular fatty acid composition of Desulfovibrio species and its use in classification of sulfatereducing bacteria. Syst. Appl. Microbiol 15:554–566
    [Google Scholar]
  28. van Gylswyk N. O. 1980; Fusobacterium polysaccharolyticum sp. nov., a gram-negative rod from the rumen that produces butyrate and ferments cellulose and starch. J. Gen. Microbiol 116:157–163
    [Google Scholar]
  29. van Gylswyk N. O. 1995; Succiniclasticum mminis gen. nov., sp. nov., a ruminal bacterium converting succinate to propionate as the sole energyyielding mechanism. Int. J. Syst. Bacteriol 45:297–300
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-2-559
Loading
/content/journal/ijsem/10.1099/00207713-46-2-559
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error