1887

Abstract

A molecular systematic investigation of members of the ascomycetous yeast genera , and was performed by using 18S rRNA gene sequence analysis. Our comparative sequence analysis revealed that and were closely related to one another and also to their teleomorphs, and , respectively. Together with and , these four species formed a stable and distinct phylogenetic group. The three representative species of the genus examined (viz., , and ) were found to be genealogically highly related to each other and exhibited a specific phylogenetic affinity (level of sequence similarity, approximately 99.2%) with (telemorph, species and formed a distinct phylogenetic group, which displayed a significant association with a phylogenetically coherent cluster encompassing , and four other species. In contrast to the situation with the genera and , the genus displayed very marked phylogenetic heterogeneity. , the type species of the genus , and six other species (viz., , and ) were phylogenetically intermixed with species of the genera , and In contrast, , and three varieties, along with their anamorph, , formed a highly stable monophyletic group worthy of separate generic status. and formed two distinct phylogenetic lines that did not exhibit particularly close affinity with each other or other ascomycetous yeast genera. Our phylogenetic findings are discussed in the context of the results of other genotypic and phenotypic studies.

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1996-04-01
2023-02-08
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References

  1. Boekhout T., Kurtzman C. P., O’Donnell K., Smith M. T. 1994; Phylogeny of the yeast genera Hanseniaspora (anamorph Kloeckera), Dekkera (anamorph Brettanomyces), and Eeniella as inferred from partial 26S ribosomal DNA nucleotide sequences. Int. J. Syst, Bacteriol 44:781–786
    [Google Scholar]
  2. Devereux J., Haeberli P., Smithies D. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  3. Felsenstein J. 1993 PHYLIP: phylogenetic inference package, version 3.5 University of Washington; Seattle:
    [Google Scholar]
  4. Feng D. F., Doolittle R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J. Mol. Evol 35:351–360
    [Google Scholar]
  5. Fuson G. B., Presley H. L., Phaff H. J. 1987; Deoxyribonucleic acid base sequence relatedness among members of the yeast genus Kluyveromyces. Int. J. Syst. Bacteriol 37:371–379
    [Google Scholar]
  6. Hendriks L., Goris A., Van de Peer Y., Neefs J.-M., Vancanneyt M., Kersters K., Berny J.-F., Henebert G. L., De Wachter R. 1992; Phylogenetic relationships among ascomycetes and ascomycete-like yeasts as deduced from small ribosomal subunit RNA sequences. Syst. Appl. Microbiol 15:98–104
    [Google Scholar]
  7. Hutson R. A., Thompson D. E., Collins M. D. 1993; Genetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related Clostridia as revealed by small-subunit rRNA gene sequences. FEMS Microbiol. Lett 108:103–110
    [Google Scholar]
  8. James S. A., Collins M. D., Roberts I. N. 1994; Genetic interrelationship among species of the genus Zygosaccharomyces as revealed by smallsubunit rRNA gene sequences. Yeast 10:871–881
    [Google Scholar]
  9. Johannsen E. 1980; Hybridization studies within the genus Kluyveromyces van der Walt emend, van der Walt. Antonie van Leeuwenhoek J. Microbiol 46:177–189
    [Google Scholar]
  10. Johannsen E., van der Walt J. P. 1978; Interfertility as basis for the delimitation of Kluyveromyces marxianus. Arch. Microbiol 118:45–48
    [Google Scholar]
  11. Kreger-van Rij N. J. W. 1979; A comparative ultrastructural study of the ascospores of some Saccharomyces and Kluyveromyces species. Arch. Microbiol 121:53–59
    [Google Scholar]
  12. Kurtzman C. P. 1992; rRNA sequence comparisons for assessing phylogenetic relationships among yeasts (minireview). Int. J. Syst. Bacteriol 42:1–6
    [Google Scholar]
  13. Kurtzman C. P., Robnett C. J. 1991; Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequences. Yeast 7:61–72
    [Google Scholar]
  14. Lachance M.-A. 1989; Restriction mapping of rDNA and the taxonomy of Kluyveromyces van der Walt emend, van der Walt. Yeast 5:S379–S383
    [Google Scholar]
  15. Lachance M.-A. 1993; Kluyveromyces. systematics since 1970. Antonie Leeuwenhoek 63:95–104
    [Google Scholar]
  16. Martini A., Vaughan Martini A. 1992; Assignment of Kluyveromyces cellobiovorus nomen nudum to Candida intermedia (Ciferri & Ashford) Langeron et Guerra. Antonie Leeuwenhoek 61:57–60
    [Google Scholar]
  17. Meyer S. A., Ahearn D. G., Yarrow D. 1984 Genus 4. Candida Berhout. 585–844 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  18. Molina F. I., Shen P., Jong S.-C. 1993; Validation of the species concept in the genus Dekkera by restriction analysis of genes coding for rRNA. Int. J. Syst. Bacteriol 43:32–35
    [Google Scholar]
  19. Philippsen P., Stotz A., Scherf C. 1991; DNA of Saccharomyces cerevisiae. Methods EnzymoL 194:169–182
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd.655–656 Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  21. Shen P., Jong S.-C., Molina F. I. 1994; Analysis of ribosomal DNA restriction patterns in the genus Kluyveromyces. Antonie Leeuwenhoek 65:99–105
    [Google Scholar]
  22. Sidenberg D. G., Lachance M.-C. 1986; Electrophoretic isoenzyme variation in Kluyveromyces populations and revision of Kluyveromyces marxianus (Hansen) van der Walt. Int. J. Syst. Bacteriol 36:94–102
    [Google Scholar]
  23. Sor R., Fukuhara H. 1989; Analysis of chromosomal DNA patterns of the genus Kluyveromyces. Yeast 5:1–10
    [Google Scholar]
  24. van der Walt J. P., Johannsen E. 1979; A comparison of interfertility and in vitro DNA-DNA reassociation as criteria for speciation in the genus Kluyveromyces. Antonie van Leeuwenhoek J. Microbiol 45:281–291
    [Google Scholar]
  25. van der Walt J. P., Johannsen E. 1984 Genus 13, Kluyveromyces,. 224–251 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  26. Viljoen B. C-, Kock J. L. F., Coetzee D. J. 1988; Orthogonal-fieldalternation gel electrophoresis banding patterns of some asporogenous yeasts and their respective ascosporogenous states. Syst. Appl. Microbiol 10:228–230
    [Google Scholar]
  27. Wilmotte A., Van de Peer Y., Goris A., Chapelle S., De Baere R., Nelissen B., Neefs J.-M., Hennebert G. L., De Wachter R. 1993; Evolutionary relationships among higher fungi inferred from small ribosomal subunit RNA sequence analysis. Syst. Appl. Microbiol 16:436–444
    [Google Scholar]
  28. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev 51:221–271
    [Google Scholar]
  29. Yamada Y., Maeda K., Mikata K. 1994; The phylogenetic relationships of Rhodosporidium dacryodum. Fell, Hunter et Tallman based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Sakaguchia gen. nov., a heterobasidiomycetous yeast genus. Biosci. Biotechnol. Biochem 58:99–103
    [Google Scholar]
  30. Yamada Y., Matsuda M., Maeda K., Mikata K. 1994; The phylogenetic relationships of species of the genus Dekkera van der Walt based on the partial sequences of 18S and 26S ribosomal RNAs (Saccharomycetaceae). Biosci. Biotechnol. Biochem 58:1803–1808
    [Google Scholar]
  31. Yamada Y., Nagahama T., Banno I. 1991; The molecular phylogeny of the Q9-equipped ascomycetous teleomorphic yeast genus Debaryomyces Lodder et Kreger-van Rij based on the partial sequences of 18S and 26S ribosomal ribonucleic acids. J. Gen. Appl. Microbiol 37:277–288
    [Google Scholar]
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