Partial nucleotide sequences of the genes (DNA gyrase B subunit genes) of 15 strains, including the type and reference strains of genomic species 1 to 12 ( [genomic species 1], [genomic species 2], genomic species 3, [genomic species 4], [genomic species 5], genomic species 6, [genomic species 7], [genomic species 8], genomic species 9, genomic species 10, genomic species 11, and [genomic species 12]), were determined by sequencing the PCR-amplified fragments of . The sequence homology among these strains ranged from 69.6 to 99.7%. A phylogenetic analysis, using the sequences, indicates that genomic species 1, 2, and 3 formed one cluster (87.3 to 90.3% identity), while genomic species 8 and 9 formed another cluster (99.7% identity). These results are consistent with those of DNA-DNA hybridization and of biochemical systematics. On the other hand, the topology of the published phylogenetic tree based on the 16S rRNA sequences of the strains was quite different from that of the -based tree. The numbers of substitution in the 16S rRNA gene sequences were not high enough to construct a reliable phylogenetic tree. The -based analysis indicates that the genus is highly diverse and that a reclassification of this genus would be required.


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