RT Journal Article SR Electronic(1) A1 YOON, JUNG-HOON A1 KIM, HONGIK A1 KIM, SAM-BONG A1 KIM, HONG-JOONG A1 KIM, WON YONG A1 LEE, SUNG TAIK A1 GOODFELLOW, MICHAEL A1 PARK, YONG-HAYR 1996 T1 Identification of Saccharomonospora Strains by the Use of Genomic DNA Fragments and rRNA Gene Probes JF International Journal of Systematic and Evolutionary Microbiology, VO 46 IS 2 SP 502 OP 505 DO https://doi.org/10.1099/00207713-46-2-502 PB Microbiology Society, SN 1466-5034, AB Restriction digestion fragments of DNAs extracted from 14 representative strains of Saccharomonospora azurea, “Saccharomonospora caesia,” Saccharomonospora cyanea, Saccharomonospora glauca, and Saccharomonospora viridis and six “Saccharomonospora”-like isolates were separated by electrophoresis, Southern blotted onto nylon membranes, and hybridized by using two rRNA gene probes cloned from Streptomyces griseus subsp. griseus KCTC 9080. The following four restriction endonucleases were used: Bam HI, Sal I, Pvu II, and Xho I. The resultant five ribotype patterns were considered species specific. The genomic diversity revealed by ribotyping indicates that this method can be used to both characterize and identify saccharomonosporae. All of the test strains contained DNA with three rRNA gene clusters., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-46-2-502