Different species may be identified by using polymorphisms in their glutamine synthetases (GSII) but not by their GSI profiles. We analyzed the GSs of various and strains (which are capable of nodulating and fixing nitrogen in beans), as well as strains of other species included for comparison. The GS polymorphisms were determined by identifying variations in native enzyme mobility (revealed by GS activity staining) and in the isoelectric points of the monomers (revealed by immunodetection with antibodies against the GS proteins) by using gel electrophoresis. Restriction fragment length polymorphism patterns obtained by hybridizing an internal fragment of the GSII gene obtained from with total fragmented DNAs from different strains clearly distinguished the different groups. GSII is a novel and useful marker for groups and species, and GSII data support and as bona fide species.


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