1887

Abstract

We determined nearly complete flagellin gene sequences for sensu lato isolates (11 isolates obtained from ticks and patients in Hokkaido, Japan, and 1 European isolate) representing six different restriction fragment length polymorphism (RFLP) ribotype groups following cloning of the PCR-amplified genes. These sequences were aligned with those of representatives of the three species associated with Lyme disease, and a phylogenetic tree was construced by the Clustal method. On the Lyme disease borrelia portion of the tree, the species were clearly delineated into three different phylogenetic groups, in complete agreement with the division of sensu lato into three species. A phylogenetic analysis revealed that the representatives of RFLP ribotype groups IV, V, and VI clustered tightly with each other and belonged on the same branch as . We used the criteria that are currently used to delineate bacterial species and determined the levels of DNA relatedness for these isolates. For the RFLP ribotype group IV, V, and VI isolates, the levels of DNA relatedness ranged from 79 to 88%, and the levels of relatedness to the reference strain of ranged from 70 to 80%. The levels of DNA relatedness of the RFLP ribotype group IV, V, and VI isolates to the representatives of other species associated with Lyme disease ranged from 53 to 66%. All of these findings indicate that the RFLP ribotype group IV, V, and VI isolates should be included in the species .

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1996-04-01
2024-12-05
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References

  1. Anderson J. F. 1989; Epizootiology of Borrelia in Ixodes tick vectors and reservoir hosts. Rev. Infect. Dis 11:Suppl. 6sl451–sl459
    [Google Scholar]
  2. Anderson J. F., Duray P. H., Magnarelli L. A. 1987; Prevalence of Borrelia burgdorferi in white-footed mice and Ixodes dammini at Fort McCoy, Wis. J. Clin. Microbiol 25:1495–1497
    [Google Scholar]
  3. Assous M. V., Postic D., Paul G., Nevot P., Baranton G. 1994; Individualisation of two new genomic groups among American Borrelia burgdorferi sensu lato strains. FEMS Microbiol. Lett 121:93–98
    [Google Scholar]
  4. Baba S., Hao T., Fujita H., Fujino K., Mizoguchi Y., Yoshikawa K., Suzuki H., Woo L. C., Kubo N., Arashima Y., Kawano K., Iguchi K. 1990; Lyme borreliosis-two subclinical cases. Rinsho Hifuka 44:483–486
    [Google Scholar]
  5. Baranton G., Postic D., Saint Girons I., Boerlin P., Piffaretti J. C., Assous M., Grimont P. A. D. 1992; Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol 42:378–383
    [Google Scholar]
  6. Burgdorfer W. 1984; Discovery of the Lyme disease spirochete and its relationship to tick vectors. Yale J. Biol. Med 57:71–76
    [Google Scholar]
  7. Buidorfer W., Haynes S. F., Corwin D. 1989; Pathophysiology of the Lyme disease spirochete, Borrelia burgdorferi, in ixodid ticks. Rev. Infect. Dis 11:Suppl. 6sl442–sl450
    [Google Scholar]
  8. Canica M. M., Nato F., Merle L. du, Mazie J. C., Baranton G., Postic D. 1993; Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand. J. Infect. Dis 25:441–448
    [Google Scholar]
  9. Fukunaga M., Hamase A. 1995; Outer surface protein C gene sequence analysis of Borrelia burgdorferi sensu lato isolates of Japan. J. Clin. Microbiol 33:2415–2420
    [Google Scholar]
  10. Fukunaga M., Mifuchi I. 1989; Unique organization of Leptospira interrogans rRNA genes. J. Bacteriol 171:5763–5767
    [Google Scholar]
  11. Fukunaga M., Sohnaka M. 1992; Tandem repeat of the 23s and 5S ribosomal RNA genes in Borrelia burgdorferi, the etiological agent of Lyme disease. Biochem. Biophys. Res. Commun 183:952–957
    [Google Scholar]
  12. Fukunaga M., Sohnaka M., Nakao M., Miyamoto K. 1993; Evaluation of genetic divergence of borrelial isolates from Lyme disease patients in Hokkaido, Japan, by rRNA gene probes. J. Clin. Microbiol 31:2044–2048
    [Google Scholar]
  13. Fukunaga M., Sohnaka M., Takahashi Y., Nakao M., Miyamoto K. 1993; Antigenic and genetic characterization of Borrelia species isolated from Ixodes persulcatus in Hokkaido, Japan. J. Clin. Microbiol 31:1388–1391
    [Google Scholar]
  14. Fukunaga M., Sohnaka M., Yanagihara Y. 1993; Analysis of Borrelia species associated with Lyme disease by rRNA gene restriction fragment length polymorphism. J. Gen. Microbiol 139:1141–1146
    [Google Scholar]
  15. Fukunaga M., Takahashi Y. 1994; Pulsed field gene electrophoresis analysis of Borrelia burgdorferi sensu lato isolated in Japan and taxonomic implications with Lyme disease spirochetes. Microbiol. Immunol 38:747–751
    [Google Scholar]
  16. Fukunaga M., Takahashi Y., Tsuruta Y., Matsushita O., Ralph D., McClelland M., Nakao M. 1995; Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus, the vector for Lyme disease in Japan. Int. J, Syst. Bacteriol 45:804–810
    [Google Scholar]
  17. Fukunaga M., Yanagihara Y., Sohnaka M. 1992; The 23S/5S ribosomal RNA genes (rrl/nf) are separate from the 16S ribosomal RNA gene (rrs) in Borrelia burgdorferi, the aetiological agent of Lyme disease. J. Gen. Microbiol 138:871–877
    [Google Scholar]
  18. Gassmann G. S., Jacobs E., Deutzmann R., Gobel U. B. 1991; Analysis of the Borrelia burgdoiferi GeHo/7a gene and antigenic characterization of its gene product. J. Bacteriol 173:1452–1459
    [Google Scholar]
  19. Higgines D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comput. Appl. Biosci 8:189–191
    [Google Scholar]
  20. Jaenson T. G. T. 1991; The epidemiology of Lyme borreliosis. Parasitol. Today 7:39–45
    [Google Scholar]
  21. Johnson R. C., Hyde F. W., Schmid G. P., Brenner D. J. 1984; Borrelia burgdorferi sp. nov, etiological agent of Lyme disease. Int. J. Syst. BacterioL 34:496–497
    [Google Scholar]
  22. Kawabata M., Baba S., Iguchi K., Yamaguchi N., Russell H. 1987; Lyme disease in Japan and its possible incriminated tick vector, Ixodes persulcatus. J. Infect. Dis 156:854
    [Google Scholar]
  23. Livey I., Gibbs C. P., Schuster R., Dorner F. 1995; Evidence for lateral transfer and recombination in OspC variation in Lyme disease borrelia. Mol. Microbiol 18:257–269
    [Google Scholar]
  24. Marconi R. T., Garon C. F. 1992; Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16S rRNA signature nucleotide analysis. J. Clin. Microbiol 30:2830–2834
    [Google Scholar]
  25. Meyer S. A., Scleifer K. H. 1978; Deoxyribonucleic acid reassociation in the classification of coagulase positive staphylococci. Arch. Microbiol 117:183–188
    [Google Scholar]
  26. Miyamoto K., Nakao M., Sato N., Mori M. 1991; Isolation of Lyme disease spirochetes from an ixodid tick in Hokkaido, Japan. Acta Trop 49:65–68
    [Google Scholar]
  27. Miyamoto K., Nakao M., Uchikawa K., Fujita H. 1992; Prevalence of Lyme borreliosis spirochetes in ixodid ticks of Japan, with special reference to a new potential vector, Ixodes ovatus (Acari: Ixodidae). J. Med. Entomol 29:216–220
    [Google Scholar]
  28. Miyamoto K., Takahashi K., Sato N., Uraguchi K., Matsuo K., Iizuka J., Mori M., Tsuboi Y., Ohtsuka K. 1990; Cases of erythema and Lyme disease associated with tick-bite in Hokkaido. Jpn. J. Sanit. Zool 41:63–65
    [Google Scholar]
  29. Nakao M., Miyamoto K. 1993; Reservoir competence of the wood mouse, Apodemus speciosus ainu, for the Lyme disease spirochete, Botrelia burgdorferi, in Hokkaido, Japan. Jpn. J. Sanit. Zool 44:69–84
    [Google Scholar]
  30. Nakao M., Miyamoto IC, Fukunaga M. 1994; Lyme disease spirochetes in Japan: enzootic transmission cycles in birds, rodents, and Ixodes persulcatus ticks. J. Infect. Dis 170:878–882
    [Google Scholar]
  31. Nakao M., Miyamoto K., Fukunaga M., Hashimoto Y., Takahashi H. 1994; Comparative studies on Borrelia afzelii isolated from a patient of Lyme disease, Ixodes persulcatus ticks, and Apodemus speciosus rodents in Japan. Microbiol. Immunol 38:413–420
    [Google Scholar]
  32. Nakao M., Miyamoto K., Kawaguchi N., Hashimoto Y., Iizuka H. 1992; Comparison of Borrelia burgdorferi isolated from humans and ixodid ticks in Hokkaido, Japan. Microbiol. Immunol 36:1189–1193
    [Google Scholar]
  33. Nakao M., Miyamoto K., Uchikawa K., Fujita H. 1992; Characterization of Borrelia burgdorferi isolated from Ixodes persulcatus and Ixodes ovatus ticks in Japan. Am. J. Trop. Med. Hyg 47:505–511
    [Google Scholar]
  34. Noppa L., Burman N., Sadziene A., Barbour A. G., Bergstrom S. 1995; Expression of the flagellin gene in Borrelia is controlled by an alternative p factor. Microbiology 141:85–93
    [Google Scholar]
  35. Park K. H., Chang W. H., Schwan T. G. 1993; Identification and characterization of Lyme disease spirochetes, Borrelia burgdorferi sensu lato, isolated in Korea. J. Clin. Microbiol 31:1831–1837
    [Google Scholar]
  36. Picken R. N. 1992; Polymerase chain reaction primers and probes derived from flagellin gene sequences for specific detection of the agents of Lyme disease and North American relapsing fever. J. Clin. Microbiol 30:99–114
    [Google Scholar]
  37. Postic D., Assous M. V., Grimont P. A. D., Baran ton G. 1994; Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol 44:743–752
    [Google Scholar]
  38. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  39. Southern E. M. 1975; Detection of specific sequence among DNA fragments separated by gel electrophoresis. J. Mol. Biol 98:503–517
    [Google Scholar]
  40. Takahashi Y., Sohnaka M., Nakao M., Miyamoto K., Fukunaga M. 1993; Characterization of Borrelia species isolated from ixodid ticks, Ixodes ovatus. Microbiol. Immunol 37:721–727
    [Google Scholar]
  41. Wallich R., Helmes C., Schaible U. E., Lobet Y., Moter S. E., Kramer M. D., Simon M. M. 1992; Evaluation of genetic divergence among Borrelia burgdorferi isolates by use of OspA, fla, HSP60, and HSP70 gene probes. Infect. Immun 60:4856–4866
    [Google Scholar]
  42. Wilske B., Preac-Mursic V., Gobel U. B., Graf B., Jauris S., Soutschek E., Schwab E., Zumstein G. 1993; An OspA serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J. Clin. Microbiol 31:340–350
    [Google Scholar]
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