We examined aerobic, thermophilic, gram-negative bacteria that were isolated from hot springs in Portugal and were identified as strains and placed in phenetic groups on the basis of their phenotypic characteristics. We determined the composition of the peptidoglycan, identified the respiratory quinones, and determined the mean base composition of the DNA, and the levels of DNA-DNA homology were determined by both the filter hybridization and reassociation rate methods. , and were not detected in this collection of organisms, although three strains were identified. We propose that the isolates that belonged to phenetic clusters E and F are members of a new species, ; the type strain of is strain SPS17.


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