1887

Abstract

Five chitin-degrading bacteria were isolated from soil. These organisms were strictly aerobic and rod shaped, formed spores, contained menaquinone 7 as the major isoprenoid quinone and 12-methyltetradecanoic acid as the major cellular fatty acid, and had guanine-plus-cytosine contents of 51.3 to 54.9 mol%, characteristics which indicate that they belong to the genus . These five strains were divided into two groups on the basis of physiological characteristics and the results of a DNA-DNA hybridization study. As low levels of DNA relatedness were found between our isolates and previously described strains, we propose that our isolates should be classified in two new species, and . The type strains of and are strains IFO 15659 and IFO 15660, respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-1-76
1996-01-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/1/ijs-46-1-76.html?itemId=/content/journal/ijsem/10.1099/00207713-46-1-76&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Baumann P., Gauthier M. J., Baumann L. 1984 Genus Alteromonas Baumann, Baumann, Mandel and Allern 1972. 343–352 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  3. Baumann P., Schubert R. H. W. 1984 Family IL Vibrionaceae Veron 1965. 516–550 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Berger L. R., Reynolds D. M. 1958; The chitinase system of a strain of Streptomyces griseus. Biochim. Biophys. Acta 29:522–534
    [Google Scholar]
  5. Claus D., Berkeley R. C. W. 1986 Genus Bacillus Cohn 1872. 1105–1139 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  6. Cody R. M. 1989; Distribution of chitinase and chitobiase in Bacillus. Curr. Microbiol 19:201–205
    [Google Scholar]
  7. Cowan S. T., Steel K. J. 1965 Manual for the identification of medical bacteria Cambridge University Press; London:
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol 39:224–229
    [Google Scholar]
  9. Farrow J. A. E., Ash C., Wallbanks S., Collins M. D. 1992; Phylogenetic analysis of the genera Pianococcus, Marinococcus and Sporosarcina and their relationships to members of the genus Bacillus. FEMS Microbiol. Lett 93:167–172
    [Google Scholar]
  10. Goodfellow M. 1992 The family Streptosporangiaceae,. 1115–1138 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. IL Springer-Verlag; New York:
    [Google Scholar]
  11. Gordon R. E., Haynes W. C., Pang C. H.-N. 1973 The genus Bacillus. Agriculture Handbook No. 427. U.S. Government Printing Office; Washington, D.C:
    [Google Scholar]
  12. Jeuniaux C. 1966; [III]Chitinase. Methods Enzymol 8:644–650
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol 39:159–167
    [Google Scholar]
  14. Miller J. H. 1972 Experiments in molecular genetics Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y:
    [Google Scholar]
  15. Molise E. M., Drake C. H. 1973; Chitinolysis by serratiae, including Serratia liquefaciens (Enterobacter liquefaciens). Int. J. Syst. Bacteriol 23:278–280
    [Google Scholar]
  16. Monreal J., Reese E. T. 1969; The chitinase of Serratia marcescens. Can. J. Microbiol 15:689–696
    [Google Scholar]
  17. Nakamura L. K. 1984; Bacillus amylofyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev., Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev. Int. J. Syst. Bacteriol 34:224–226
    [Google Scholar]
  18. Nakamura L. K. 1990; Bacillus thiaminofyticus sp. nov., nom. rev. Int. J. Syst. Bacteriol 40:242–246
    [Google Scholar]
  19. Nakamura L. K. 1993; DNA relatedness of Bacillus brevis Migula 1900 strains and proposal of Bacillus agri sp. nov., nom. rev., and Bacillus centrosporus sp. nov., nom. rev. Int. J. Syst. Bacteriol 43:20–25
    [Google Scholar]
  20. Nakamura L. K., Swezey J. 1983; Deoxyribonucleic acid relatedness of Bacillus circulans Jordan 1890 strains. Int. J. Syst. Bacteriol 33:703–708
    [Google Scholar]
  21. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus. J. Gen. Microbiol 134:1847–1882
    [Google Scholar]
  22. Reichenbach H. 1989 Order 1. Cytophagales Leadbetter 1974. 2011–2083 Staley J. T., Bryant M. P., Pfennig N., Holt J. G.ed Bergey’s manual of systematic bacteriology 3 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  23. Reichenbach H. 1992 The genus Lysobacter,. 3256–3275 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. IV Springer-Verlag; New York:
    [Google Scholar]
  24. Reichenbach H. 1992 The genus Herpetosiphon,. 3785–3805 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H.ed The prokaryotes, 2nd. IV Springer-Verlag; New York:
    [Google Scholar]
  25. Robbins P. W., Albright C., Benfield B. 1988; Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. J. Biol. Chern 263:443–447
    [Google Scholar]
  26. Saito H., Miuya K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619–629
    [Google Scholar]
  27. Sangkhobol V., Skerman V. B. D. 1981; Chitinophaga, a new genus of chitinolytic myxobacteria. Int. J. Syst. Bacteriol 31:285–293
    [Google Scholar]
  28. Shida O., Takagi H., Kadowaki K., Udaka S., Nakamura L. K., Komagata K. 1995; Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., an,d Bacillus borstelensis sp. nov., nom. rev. Int. J. Syst. Bacteriol 45:93–100
    [Google Scholar]
  29. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int. J. Syst. Bacteriol 33:188–193
    [Google Scholar]
  30. Takagi H., Shida O., Kadowaki K., Komagata K., Udaka S. 1993; Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov. Int. J. Syst. Bacteriol 43:221–231
    [Google Scholar]
  31. Watanabe T., Oyanagi W., Suzuki K., Tanaka H. 1990; Chitinase system of Bacillus circulans WL-12 and importance of chitinase Al in chitin degradation. J. Bacteriol 172:4017–4022
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-46-1-76
Loading
/content/journal/ijsem/10.1099/00207713-46-1-76
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error