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Five chitin-degrading bacteria were isolated from soil. These organisms were strictly aerobic and rod shaped, formed spores, contained menaquinone 7 as the major isoprenoid quinone and 12-methyltetradecanoic acid as the major cellular fatty acid, and had guanine-plus-cytosine contents of 51.3 to 54.9 mol%, characteristics which indicate that they belong to the genus Bacillus. These five strains were divided into two groups on the basis of physiological characteristics and the results of a DNA-DNA hybridization study. As low levels of DNA relatedness were found between our isolates and previously described Bacillus strains, we propose that our isolates should be classified in two new Bacillus species, Bacillus ehimensis and Bacillus chitinolyticus. The type strains of B. ehimensis and B. chitinolyticus are strains IFO 15659 and IFO 15660, respectively.
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