1887

Abstract

In order to develop a PCR method to detect in human feces and to clarify the phylogenetic position of this species, its 16S rRNA gene sequence was determined. The sequence described in this paper is different from the 16S rRNA gene sequence of in the GenBank database (accession number M58682). A PCR assay based on the new sequence is specific for , and the results of this assay confirmed that is the most common species in human feces. However, a PCR assay based on the original GenBank sequence was negative when it was performed with two strains of obtained from the American Type Culture Collection. A phylogenetic tree based on the new 16S rRNA gene sequence was constructed. On this tree was not a member of the group but was closer to some spp. and located between “clusters III and IV” (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812–826, 1994).

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1996-01-01
2022-05-20
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