We studied five strains of a new taxon recently identified among strains associated with invasive diseases (R. J. Wallace, Jr., B. A. Brown, Z. Blacklock, R. Ulrich, K. Jost, J. M. Brown, M. M. McNeil, G. Onyi, V. A. Steingrube, and J. Gibson, J. Clin. Microbiol. 33:1528-1533, 1995) to determine their taxonomic status. Several characteristics of these organisms, including the presence of chemotype IV cell walls, nocardomycolic acids, a predominant menaquinone similar to that of ATCC 19247 (T = type strain), and G+C contents ranging from 67 to 68 mol%, are characteristics of the genus . Phylogenies based on small-subunit ribosomal DNA sequences clearly confirmed that all five strains belong to the genus and occur on a single branch that is clearly distinct from . This branch forms a clade with , and . The five new strains exhibited high levels of DNA relatedness with each other, as determined by DNA-DNA hybridization experiments (S1 nuclease procedure), but not with strains or with strains of the four phylogenetically related species mentioned above. The five new strains differ from in the following characteristics: mycolic acid pattern, decomposition of adenine, nitrate reduction, and antimicrobial agent susceptibilities. Therefore, we propose that these strains belong to a new species, . The type strain is strain ATCC 51512, which was isolated from a leg abscess on a patient suffering from ulcerative colitis.


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