* Corresponding author. Mailing address: Microbial Diseases Research Laboratory, Bldg. 304, Room E3-224, VAMC West Los Angeles 691/151J, Los Angeles, CA 90073. Phone: (310) 268-3404. Fax: (310) 268-4646.
Campylobacter gracilis (formerly Bacteroides gracilis) is an asaccharolytic, nitrate-positive, urease-negative organism that requires formate and fumarate or hydrogen as a growth additive and may pit agar media. Clinical isolates that were obtained primarily from appendiceal and peritoneal fluid specimens and initially were identified in our laboratory as B. gracilis were later found to include “unusual” strains that could be distinguished by biochemical and genetic criteria. These unusual C. gracilis strains were bile resistant, could not reduce tetrazolium chloride under aerobic conditions if formate and fumarate were added to the medium, and could grow in the presence of 2 or 6% oxygen if no blood was added to the medium. C. gracilis, other campylobacters, and the unusual strains produced distinctive dehydrogenase patterns when gels were incubated anaerobically. A cellular fatty acid analysis revealed that the cluster formed by the unusual organisms was distinct from the (separate) clusters formed by C. gracilis, Bacteroides ureolyticus, and other Campylobacter species. 16S rRNA sequence data indicated that these organisms are not related phylogenetically to either C. gracilis or other Campylobacter species; the most closely related taxa as determined by rRNA sequence analysis were unrelated aerobes (members of the genera Bordetella, Alcaligenes, Rhodocyclus, and Comamonas). DNA homology data confirmed that these taxa are separate groups. Our data indicate that the unusual organisms are members of a new genus and new species, for which we propose the name Sutterella wadsworthensis. The type strain of S. wadsworthensis is strain WAL 9799 (= ATCC 51579).
EtohY., DewhirstF. E., PasterB. J., YamamotoA., GotoN.1993; Campylobacter showae sp. nov., isolated from the human oral cavity. Int. J. Syst. BacterioL 43:631–639
FrothinghamR., AllenR. L., WilsonK. H.1991; Rapid 16S ribosomal DNA sequencing from a single colony without DNA extraction or purification. BioFeedback 11:40–44
HanY.-H., SmibertR. M., KriegN. R.1991; Wolinella recta, Wolinella ciirva, Bacteroides ureolyticus, and Bacteroides gracilis are microaerophiles, not anaerobes. Int. J. Syst. Bacteriol 41:218–222
JohnsonC. C., ReinhardtJ. F., EdelsteinM. A., MulliganM. E., GeorgeW. L., FinegoldS. M.1985; Bacteroides gracilis, an important anaerobic bacterial pathogen. J. Clin. Microbiol 22:799–802
MolitorisE., FinegoldS. M., WexlerH.1991 Use of triphenytetrazolium chloride (TTC) to facilitate determination of ceftizoxime (ZOX) MICs against anaerobes, abstr. A25. 5Abstracts of the 91st General Meeting of the American Society for Microbiology 1991 American Society for Microbiology; Washington, D.C.:
MossC. W., Nunez-MontielO. L.1982; Analysis of short-chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column. J. Clin. Microbiol 15:308–311
Popovic-UroicT., PattonC. M., NicholsonM. A., KiehlbauchJ. A.1990; Evaluation of the indoxyl acetate hydrolysis test for rapid differentiation of Campylobacter, Helicobacter, and Wolinella species. J. Clin. Microbiol 28:2335–2339
ShahH.j, GharbiaS. E.1992; Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol 42:542–546
ShahH. N., CollinsM. D.1989; Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int. J. Syst. Bacteriol 39:85–87
TannerA. C. R., BadgerS., LaiC. H., ListgartenM. A., ViscontiR. A., SocranskyS. S.1981; Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb, nov., and description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from humans with periodontal disease. Int. J. Syst. Bacteriol 31:432–445
VandammeP., DaneshvarM. L., DewhirstF. E., PasterB. J., KerstersK., GoossensH., MossC. W.1995; Chemotaxonomic analyses of Bacteroides gracilis and Bacteroides ureolyticus and reclassification of B. gracilis as Campylobacter gracilis comb. nov. Int. J. Syst. Bacteriol 45:145–152
WhileyR A., BeightonD.1991; Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus as distinct species. Int. J. Syst. Bacteriol 41:1–5
WilsonK. H., BlitchingtonR., HindenachB., GreeneR. C.1988; Species-specific oligonucleotide probes for rRNA of Clostridium difficile and related species. J. Clin. Microbiol 26:2484–2488