1887

Abstract

Analysis of the genomic DNAs of chlamydial isolates from sheep, cattle, and pigs was performed by Southern blot hybridization and by restriction endonuclease (RE) profiling of DNA amplified by PCR. The hybridization probes were derived from whole genomic DNA, the major outer membrane protein (MOMP) gene, the 16S rRNA gene, and an avian isolate plasmid. The PCR analysis used targets in the MOMP gene, the 16S rRNA gene, and the 60-kDa cysteine-rich protein gene. Together, the results showed that although there was considerable heterogeneity in the DNA sequence in the MOMP gene region, all the isolates had the same underlying total genomic RE profiles and yielded identical RE profiles for the rRNA and 60-kDa-protein gene regions. Most of the isolates were found to hybridize with the plasmid probe. Comparison of the MOMP sequence of one of the isolates (P787) with that of a known strain together with the results of the RE analyses allowed the conclusion that the isolates should all be classified within this new species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-46-1-245
1996-01-01
2022-09-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/46/1/ijs-46-1-245.html?itemId=/content/journal/ijsem/10.1099/00207713-46-1-245&mimeType=html&fmt=ahah

References

  1. Andersen A. A., Tappe J. P. 1989; Genetic, immunologic and pathologic characterisation of avian chlamydial strains. J. Am. Vet. Med. Assoc 195:1512–1516
    [Google Scholar]
  2. Anderson I. E., Baxter T. A. 1986; Chlamydia psittaci’. inclusion morphology in cell culture and virulence in mice of ovine isolates. Vet. Rec 119:453–454
    [Google Scholar]
  3. Baxter S. I. F. 1990; Development of molecular techniques for the detection of Chlamydia psittaci infection in sheep and the typing of chlamydial isolates. Ph.D. thesis University of Edinburgh; Edinburgh:
    [Google Scholar]
  4. Carter M. W., Al-Mahdawi A. H., Giles I. G., Trehame J. D., Ward M. E., Clarke I. N. 1991; Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207. J. Gen. Microbiol 137:465–475
    [Google Scholar]
  5. Cox R. L., Kuo C.-C., Grayston J. T., Campbell L. A. 1988; Deoxyribonucleic acid relatedness of Chlamydia sp. isolate TWAR to Chlamydia trachomatis and Chlamydia psittaci. Int. J. Syst. BacterioL 38:265–268
    [Google Scholar]
  6. Denamur E., Sayada C., Souriau A., Orfila J., Rodolakis A., Elion J. 1991; Restriction pattern of the major outer membrane protein gene provides evidence for a homogeneous invasive group among ruminant isolates of Chlamydia psittaci. J. Gen. Microbiol 137:2525–2530
    [Google Scholar]
  7. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling to high specific activity. Anal. Biochem 132:6–13
    [Google Scholar]
  8. Frost E. H., Deslandes S., Bourgaux-Ramoisy D. 1990 Typing Chlamydia isolates with the polymerase chain reaction. 499–502 Bowie W. R. et al.ed Chlamydial infections Cambridge University Press; Cambridge:
    [Google Scholar]
  9. Fukushi H., Hirai K. 1989; Genetic diversity of avian and mammalian Chlamydia psittaci isolates and relation to host origin. J. BacterioL 171:2850–2855
    [Google Scholar]
  10. Fukushi H., Hirai K. 1992; Proposal of Chlamydia pecorum sp. nov. for Chlamydia isolates derived from ruminants, Int. J. Syst. BacterioL 42:306–308
    [Google Scholar]
  11. Fukushi H., Hirai K. 1993; Restriction fragment length polymorphisms of rRNA as genetic markers to differentiate Chlamydia spp. Int. J. Syst. BacterioL 43:613–617
    [Google Scholar]
  12. Fukushi H., Hirai K. 1993; Chlamydia pecorum–the fourth species of the genus Chlamydia. Microbiol. Immunol 37:515–522
    [Google Scholar]
  13. Gaydos C. A., Palmer L., Quinn T. C., Falkow S., Brooks G. F., Eiden J. J. 1993; Phylogenetic relationship of Chlamydia pneumoniae to Chlamydia psittaci and Chlamydia trachomatis as determined by analysis of 16S ribosomal DNA sequences. Int. J. Syst. BacterioL 43:610–612
    [Google Scholar]
  14. Griffiths P. C., Philips H. L., Dawson M., Clarkson M. J. 1992; Antigenic and morphological differentiation of placental and intestinal isolates of Chlamydia psittaci of ovine origin. Vet. Microbiol 30:165–177
    [Google Scholar]
  15. Herring A. J. 1993; Typing Chlamydia psittaci–a review of methods and recent findings. Br. Vet. J 159:455–475
    [Google Scholar]
  16. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. 1982; Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J. Clin. Microbiol 16:473–477
    [Google Scholar]
  17. Herring A. J., Tan T. W., Baxter S. 1990 Chlamydial abortion in sheep: molecular approaches to vaccination, pathogen detection and isolate typing. 378–382 Bowie W. R. et al.ed Chlamydial infections Cambridge University Press; Cambridge:
    [Google Scholar]
  18. Herring A. J., Tan T. W., Baxter S., Inglis N. F., Dunbar S. 1989; Sequence analysis of the major outer membrane protein gene of an ovine abortion isolate of Chlamydia psittaci. FEMS Microbiol. Lett 65:153–158
    [Google Scholar]
  19. Hugall A., Timms P., Girges A. A., Lavin M. F. 1989; Conserved sequences in chlamydial plasmids. Plasmid 22:91–98
    [Google Scholar]
  20. Johnston J. L. 1984 Nucleic acids in bacterial classification. 8–11 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 Williams & Wilkins; Baltimore:
    [Google Scholar]
  21. Kaltenboeck B., Kousoulas K., Storz J. 1992; Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp. J. Clin. Microbiol 30:1098–1104
    [Google Scholar]
  22. Kaltenboeck K., Kousoulas G., Storz J. 1993; Structures of and allelic diversity and relationships among the major outer membrane protein (pmpA) genes of the four chlamydial species. J. BacterioL 175:487–501
    [Google Scholar]
  23. Kaltenboeck B., Storz J. 1992; Biological properties and genetic analysis of the ompA locus in chlamydiae isolated from swine. Am. J. Vet. Res 53:1582–1587
    [Google Scholar]
  24. Kuroda-Kitagawa Y., Suzuki-Muramatsu C., Yamaguchi T., Fukushi H., Hirai K. 1993; Antigenic analysis of Chlamydia pecorum and mammalian Chlamydia psittaci by use of monoclonal antibodies to the major outer membrane protein and to 56 to 64 kD protein. Am. J. Vet. Res 54:709–712
    [Google Scholar]
  25. Lusher M., Storey C. C., Richmond S. J. 1989; Plasmid diversity within the genus Chlamydia. J. Gen. Microbiol 135:1145–1151
    [Google Scholar]
  26. Markey B. K., McNulty M. S., Todd D., Mackie D. P. 1993; Comparison of ovine abortion and non-abortion isolates of Chlamydia psittaci using inclusion morphology, polyacrylamide gel electrophoresis, restriction endonuclease analysis and reactivity with monoclonal antibodies. Vet. Microbiol 35:151–159
    [Google Scholar]
  27. McClenaghan M., Herring A. J., Aitken I. D. 1984; Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect. Immun 45:384–389
    [Google Scholar]
  28. McClenaghan M., Honeycombe J. R., Bevan B. J., Herring A. J. 1988; Distribution of plasmid sequences in avian and mammalian isolates of Chlamydia psittaci. J. Gen. Microbiol 134:559–565
    [Google Scholar]
  29. McClenaghan M., Inglis N. F., Herring A. J. 1991; Comparison of isolates of Chlamydia psittaci of ovine, avian and feline origin by analysis of polypeptide profiles from purified elementary bodies. Vet. Microbiol 26:269–278
    [Google Scholar]
  30. Perez-Martinez J. A., Storz J. 1985; Antigenic diversity of Chlamydia psittaci of mammalian origin determined by microimmunofluorescence. Infect Immun 50:905–910
    [Google Scholar]
  31. Rodolakis A., Bernard F., Lantier F. 1989; Mouse models for evaluation of virulence of Chlamydia psittaci isolated from ruminants. Res. Vet. Sci 46:34–39
    [Google Scholar]
  32. Rodolakis A., Souriau A. 1992; Restriction endonuclease analysis of DNA from ruminant Chlamydia psittaci and its relation to mouse virulence. Vet. Microbiol 31:263–271
    [Google Scholar]
  33. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horne G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  34. Salinas J., Souriau A., Cuello F., Rodolakis A. 1995; Antigenic diversity of ruminant Chlamydia psittaci strains demonstrated by the indirect microimmunofluorescence test with monoclonal antibodies. Vet. Microbiol 43:219–226
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  37. Schachter J., Banks J., Sugg N., Sung M., Storz J., Meyer K. F. 1974; Serotyping of Chlamydia. I. Isolates of ovine origin. Infect. Immun 9:92–94
    [Google Scholar]
  38. Smith G. E., Summers M. D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose of diazobenzyloxymethyl-paper. Anal. Biochem 109:123–129
    [Google Scholar]
  39. Stephens R. S., Sanches-Pescador R., Wagar E. A., Inouye C., Urdea M. S. 1987; Diversity of Chlamydia trachomatis major outer membrane characterization. J. Immunol 128:1083–1089
    [Google Scholar]
  40. Storey C., Lusher M., Richmond S. J. 1992 Use of comparative MOMP gene sequence data for subdivision of Chlamydia psittaci species. 25 Mardh P.-A. et al.ed Proceedings of the European Society for Chlamydia Research Uppsala University Centre for STD Research; Uppsala, Sweden:
    [Google Scholar]
  41. Storz J. 1988 Overview of animal diseases induced by chlamydial infections. 167–192 Barron A. L.ed The microbiology of Chlamydia CRC Press; Boca Raton, Fla:
    [Google Scholar]
  42. Timms P., Eaves F. W., Giijes A. A., Lavin M. F. 1988; Comparison of Chlamydia psittaci isolates by restriction endonuclease and DNA probe analyses. Infect. Immun 56:287–290
    [Google Scholar]
  43. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose gels. Proc. Natl. Acad. Sci. USA 76:615
    [Google Scholar]
  44. Watson M. W., Lambden P. R., Clarke I. N. 1991; Genetic diversity and identification of human infection by amplification of the chlamydial 60- kilodalton cysteine-rich outer membrane protein gene. J. Clin. Microbiol 29:1188–1193
    [Google Scholar]
  45. Weisburg W. G., Hatch T. P., Woese C. R. 1986; Eubacterial origin of chlamydiae. J. BacterioL 167:570–574
    [Google Scholar]
  46. Weisburg W. G., Hatch T., Woese C. R. 1990; Genbank submission no. M59178. Chlamydia trachomatis 16S ribosomal RNA gene.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-46-1-245
Loading
/content/journal/ijsem/10.1099/00207713-46-1-245
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error