Evidence for the Placement of the Gram-Negative Catonella morbi (Moore and Moore) and Johnsonella ignava (Moore and Moore) within the Clostridium Subphylum of the Gram-Positive Bacteria on the Basis of 16S rRNA Sequences
* Corresponding author. Mailing address: Department of Microbial Physiology, Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, United Kingdom.
Comparative 16S rRNA analysis was used to determine the phylogenetic positions of Catonella morbi and Johnsonella ignava, which are members of two monospecific genera of gram-negative anaerobic bacilli isolated from human gingival crevices. Both of these genera were found to belong to cluster XIVa (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812–826, 1994) of the Clostridium subphylum of gram-positive bacteria. Within this cluster, which contains several Clostridium, Coprococcus, Eubacterium, and Ruminococcus species, C. morbi and J. ignava formed two distinct lines that were separate from all other taxa. Our findings support the separate generic status of the genera Catonella and Johnsonella and show that these genera do not belong to the family Bacteroidaceae but instead belong to the gram-positive Clostridium subphylum.
CollinsM. D.,
LawsonP. A.,
WillemsA.,
CordobaJ. J.,
Fernandez-GarayzabalJ.,
GarciaP.,
CaiJ.,
HippeH.,
FarrowJ. A. E.1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int. J. Syst. Bacteriol 44:812–826
FarrowJ. A. E.,
LawsonP. A.,
HippeH.,
GauglitzU.,
CollinsM. D.1995; Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella (Bacteroides) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov. Int. J. Syst. Bacteriol 45:436–440
LawsonP. A.,
GharbiaS. E.,
ShahH. N.,
ClarkD. R.1989; Recognition of Fusobacterium nucleatum subgroups Fn-1, Fn-2 and Fn-3 by ribosomal RNA gene restriction patterns. FEMS Microbiol. Lett 65:41–46
MooreL. V. H.,
MooreW. E. C.1994; Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialisterpneumosintes comb. nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. Int. J. Syst. Bacteriol 44:187–192
WillemsA.,
CollinsM. D.1995; Phylogenetic placement of Dialisterpneumosintes (formerly Bacteroidespneumosintes) within the Sporomusa subbranch of the Clostridium subphylum of the gram-positive bacteria. Int. J. Syst. Bacteriol 45:403–405
WillemsA.,
CollinsM. D.1995; Reclassification of Oribaculum catoniae (Moore and Moore 1994) as Porphyromonas catoniae comb. nov. and emendation of the genus Porphyromonas. Int. J. Syst. Bacteriol 45:578–581
WillemsA.,
CollinsM. D.1995; 16S rRNA gene similarities indicate that Hallella seregens (Moore and Moore) and Mitsuokella dentalis (Haapasalo et al) are genealogically highly related and are members of the genus Prevotella (Shah and Collins): emended description of the genus Prevotella (Shah and Collins) and description of Prevotella dentalis comb. nov. Int. J. Syst. Bacteriol 45:832–836
Evidence for the Placement of the Gram-Negative Catonella morbi (Moore and Moore) and Johnsonella ignava (Moore and Moore) within the Clostridium Subphylum of the Gram-Positive Bacteria on the Basis of 16S rRNA Sequences