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Volume 45, Issue 4

Chromosomal Heterogeneity among Various Strains of Free

Abstract

The genomes of various strains were digested with several restriction enzymes, and the fragments were analyzed by pulsed-field gel electrophoresis. Polymorphism of the restriction patterns was observed. The genome sizes of the strains obtained when we added the restriction fragment sizes ranged from 1,650 to 1,910 kbp. Physical and genetic maps of 12 strains were constructed by using several DNA probes as genetic markers. The relative positions of mapped loci were conserved in most of the strains; the main differences were differences in the order and number of restriction sites and differences in the sizes of certain fragments. The distribution of viral sequences, which occur at multiple sites in the . genome and are homologous to the sequences of virus SpV1 strains R8A2B and S102, was also studied.

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Copyright © 1995 International Union of Microbiological Societies
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1995-10-01
2024-04-24
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Chromosomal Heterogeneity among Various Strains of Spiroplasma citri
Int J Syst Evol Microbiol 45, 729 (1995); https://doi.org/10.1099/00207713-45-4-729
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