1887

Abstract

Aerobic, thermophilic, gram-negative bacteria obtained from Yellowstone National Park that were placed in the genus on the basis of phenotypic data were examined by chemotaxonomic techniques to determine their peptidoglycan compositions, their respiratory quinones, their mean DNA base compositions, and their levels of DNA-DNA homology as determined by both the filter hybridization and reassociation rate methods. These isolates from hot springs included strains and strains of a new genospecies. We propose the name for this new genospecies; strain YS38 is the type strain of this taxon. A collection of 10 strains, including the type strain of which were isolated from widely separated geothermal sites, exhibited high levels of DNA-DNA homology with each other and had similar physiological properties. Therefore, we propose that the species (Oshima and Imahori) should be reestablished, with strain HB8 as the type strain.

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1995-07-01
2022-10-04
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References

  1. Alfredsson G. A., Baldursson S., Kristjansson J. K. 1985; Nutritional diversity among Thermus spp. isolated from Icelandic hot springs. Syst. Appl. Microbiol. 6:308–311
    [Google Scholar]
  2. Brock T. D., Boylen K. L. 1973; Presence of thermophilic bacteria in laundry and domestic hot-water heaters. Appl. Microbiol. 25:72–76
    [Google Scholar]
  3. Brock T. D., Freeze H. 1969; Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. J. Bacteriol. 98:289–297
    [Google Scholar]
  4. Collins M. D., Jones D. 1981; Distribution of isoprene quinone structural types in bacteria and their taxonomic implications. Microbiol. Rev. 45:316–354
    [Google Scholar]
  5. Cometta S., Sonnleitner B., Sidler W., Fiechter A. 1982; Population distribution of aerobic extremely thermophilic microorganisms in an Icelandic natural hot spring. Eur. J. Appl. Microbiol. Biotechnol. 16:151–156
    [Google Scholar]
  6. Degryse E., Glansdorff N., Pierard A. 1978; A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus . Arch. Microbiol. 117:189–196
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridisation from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  8. Embley T. M., Thomas R. H., Williams R. A. D. 1993; Reduced thermophilic bias in the 16S rDNA sequence from Thermus ruber provides further support for a relationship between Thermus and Deinococcus . Syst. Appl. Microbiol. 16:25–29
    [Google Scholar]
  9. Georganta G., Smith K. E., Williams R. A. D. 1993; DNA:DNA homology and cellular components of Thermus filiformis and other strains of Thermus from New Zealand hot springs. FEMS Microbiol. Lett. 107:145–150
    [Google Scholar]
  10. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur. J. Biochem. 12:143–153
    [Google Scholar]
  11. Hardy T. L., Holland D. O., Nayler J. H. C. 1955; One-phase solvent systems for the separation of amino acids. Anal. Chem. 27:971–973
    [Google Scholar]
  12. Hartmann R. K., Wolters J., Kroger B., Schultze S., Specht T., Erdmann V. A. 1989; Does Thermus represent another deep eubacterial branching?. Syst. Appl. Microbiol. 11:243–249
    [Google Scholar]
  13. Hensel R., Demharter W., Kandler O., Kroppenstedt R. M., Stackebrandt E. 1986; Chemotaxonomic and molecular-genetic studies of the genus Thermus: evidence for a phylogenetic relationship of Thermus aquaticus and Thermus ruber to the genus Deinococcus . Int. J. Syst. Bacteriol. 36:444–453
    [Google Scholar]
  14. Hudson J. A., Morgan H. W., Daniel R. M. 1986; A numerical classification of some Thermus isolates. J. Gen. Microbiol. 132:531–540
    [Google Scholar]
  15. Hudson J. A., Morgan H. W., Daniel R. M. 1987; Thermus filiformis sp. nov., a filamentous caldoactive bacterium. Int. J. Syst. Bacteriol. 37:431–436
    [Google Scholar]
  16. Hudson J. A., Morgan H. W., Daniel R. M. 1987; Numerical classification of some Thermus isolates from Icelandic hot springs. Syst. Appl. Microbiol. 9:218–223
    [Google Scholar]
  17. Kanasawud P., Teeyapan S., Lumyong S., Hoist O., Mattiasson B. 1992; Thermus 2S from Thai hot springs: isolation and immobilization. World J. Microbiol. Biotechnol. 8:137–140
    [Google Scholar]
  18. Koyama Y., Furukawa K. 1990; Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells. J. Bacteriol. 172:3490–3495
    [Google Scholar]
  19. Kristjansson J. K., Alfredsson G. A. 1983; Distribution of Thermus spp. in Icelandic hot springs and a thermal gradient. Appl. Environ. Microbiol. 45:1785–1789
    [Google Scholar]
  20. Kristjansson J. K., Hjorleifsdottir S., Marteinsson V. T., Alfredsson G. A. 1994; Thermus scotoductus, sp. nov., a pigment-producing thermophilic bacterium from hot tap water in Iceland and including Thermus sp. XI. Syst. Appl. Microbiol. 17:44–50
    [Google Scholar]
  21. Kristjansson J. K., Hreggvidsson G. O., Alfredsson G. A. 1986; Isolation of halotolerant Thermus spp. from submarine hot springs in Iceland. Appl. Environ. Microbiol. 52:1313–1316
    [Google Scholar]
  22. Loginova L. G., Egorova L. A. 1976; An obligately thermophilic bacterium Thermus ruber from hot springs in Kamchatka. Microbiology (Engl. Transl. Mikrobiologiya) 44:593–597
    [Google Scholar]
  23. Loginova L. G., Egorova L. A., Golovacheva R. S., Seregina L. M. 1986; Thermus ruber sp. nov. Int. J. Syst. Bacteriol. 34:498–499
    [Google Scholar]
  24. Munster M. J., Munster A. P., Woodrow J. R., Sharp R. J. 1986; Isolation and preliminary taxonomic studies of Thermus strains isolated from Yellowstone National Park, USA. J. Gen. Microbiol. 132:1677–1683
    [Google Scholar]
  25. Murzina N. V., Vorozheykina D. P., Marvienko N. I. 1988; Nucleotide sequence of Thermus thermophilus HB8 gene coding 16S rRNA. Nucleic Acids Res. 16:8172
    [Google Scholar]
  26. Neefs J.-M., Van de Peer Y., De Rijk P., Goris A., De Wachter R. 1991; Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res 19: Suppl 1987–2015
    [Google Scholar]
  27. Olsen G. J., Larsen N., Woese C. R. 1991; The Ribosomal RNA Database Project. Nucleic Acids Res 19: Suppl1 2017–2021
    [Google Scholar]
  28. Oshima T., Imahori K. 1971; Isolation of an extreme thermophile and thermostability of its transfer ribonucleic acid and ribosomes. J. Gen. Appl. Microbiol. 17:513–517
    [Google Scholar]
  29. Oshima T., Imahori K. 1974; Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa. Int. J. Syst. Bacteriol. 24:102–112
    [Google Scholar]
  30. Owen R. J., Hill L. R. 1979; The estimation of base composition, base pairing and genome sizes of bacterial DNA. Soc. Appl. Bacteriol. Tech. Ser. 14:277–296
    [Google Scholar]
  31. Pask-Hughes R., Williams R. A. D. 1975; Extremely thermophilic gram-negative bacteria from hot tap water. J. Gen. Microbiol. 88:321–328
    [Google Scholar]
  32. Pask-Hughes R. A., Williams R. A. D. 1977; Yellow-pigmented strains of Thermus spp. from Icelandic hot springs. J. Gen. Microbiol. 102:375–383
    [Google Scholar]
  33. Pask-Hughes R. A., Williams R. A. D. 1978; Cell envelope components of strains belonging to the genus Thermus . J. Gen. Microbiol. 107:65–72
    [Google Scholar]
  34. Peckova M. 1991; Properties of a hyperthermophilic bacterium (Thermus sp.) isolated from a Carlsbad spring. Folia Microbiol. 36:515–521
    [Google Scholar]
  35. Ramaley R. F., Hixson J. 1970; Isolation of a nonpigmented, thermophilic bacterium similar to Thermus aquaticus . J. Bacteriol. 103:527–528
    [Google Scholar]
  36. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviour of isomers of α-ε-diaminopimelic acid on paper chromatograms. J. Am. Chem. Soc. 77:4844–4846
    [Google Scholar]
  37. Saiki T., Kimura R., Arima K. 1972; Isolation and characterization of extremely thermophilic bacteria from hot springs. Agric. Biol. Chem. 36:2357–2366
    [Google Scholar]
  38. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular cloning: a laboratory manual,. , 2. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  39. Santos M. A., Williams R. A. D., da Costa M. S. 1990; Numerical taxonomy of Thermus isolates from hot springs in Portugal. Syst. Appl. Microbiol. 12:310–315
    [Google Scholar]
  40. Saul D. J., Rodrigo A. G., Reeves R. A., Williams L. C., Borges K. M., Morgan H. W., Bergquist P. L. 1993; Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data. Int. J. Syst. Bacteriol. 43:754–760
    [Google Scholar]
  41. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:245–292
    [Google Scholar]
  42. Sharp R. J., Williams R. A. D. 1988; Properties of Thermus ruber strains isolated from Icelandic hot springs and DNA-DNA homology of Thermus ruber and Thermus aquaticus . Appl. Environ. Microbiol. 54:2049–2053
    [Google Scholar]
  43. Skennan V. B. D., McGowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  44. Stramer S. L., Starzyk M. J. 1981; The occurrence and survival of Thermus aquaticus . Microbios 32:99–110
    [Google Scholar]
  45. Taguchi H., Yamashita M., Matsuzawa H., Ohta T. 1982; Heat stable and fructose 1,6-biphosphate activated l-lactate dehydrogenase from an extremely thermophilic bacterium. J. Biochem. 91:1343–1348
    [Google Scholar]
  46. Takase M., Horikoshi K. 1989; Purification and properties of a P-glu-cosidase from Thermus sp. Z-l. Agric. Biol. Chem. 53:559–560
    [Google Scholar]
  47. Williams R. A. D. 1975; Caldoactive and thermophilic bacteria and their thermostable proteins. Sci. Prog. 62:373–393
    [Google Scholar]
  48. Williams R. A. D. 1989; Biochemical taxonomy of the genus Thermus, . 82–97 da Costa M. S., Duarte J. C., Williams R. A. D. Microbiology of extreme environments and its potential for biotechnology Elsevier; London:
    [Google Scholar]
  49. Williams R. A. D. Unpublished data
  50. Williams R. A. D., da Costa M. S. 1992; The genus Thermus and related microorganisms,. 3745–3753 Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications Springer-Verlag; New York:
    [Google Scholar]
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