%0 Journal Article %A FARROW, J. A. E. %A LAWSON, P. A. %A HIPPE, H. %A GAUGLITZ, U. %A COLLINS, M. D. %T Phylogenetic Evidence that the Gram-Negative Nonsporulating Bacterium Tissierella (Bacteroides) praeacuta Is a Member of the Clostridium Subphylum of the Gram-Positive Bacteria and Description of Tissierella creatinini sp. nov. %D 1995 %J International Journal of Systematic and Evolutionary Microbiology, %V 45 %N 3 %P 436-440 %@ 1466-5034 %R https://doi.org/10.1099/00207713-45-3-436 %I Microbiology Society, %X The 16S rRNA gene sequence of the type strain of Tissierella praeacuta (formerly Bacteroides praeacutus) was determined by PCR direct sequencing. A comparative sequence analysis showed that T. praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and has a close phylogenetic affinity with the species that form Clostridium cluster XII (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994). Although T. praeacuta is gram negative and does not produce endospores, 16S rRNA sequence data showed that it is closely related genealogically (level of sequence similarity, 99.9%) to Clostridium hastiforme. On the basis of our results and the results of previous studies, a second species of Tissierella, Tissierella creatinini sp. nov., is described. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-45-3-436