1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 45, Issue 2

Classification of Strains on the Basis of Restriction Fragment Length Polymorphisms Free

Abstract

Restriction fragment length polymorphisms of the 16S rRNA genes of strains were studied by cleavage of the chromosomal DNA with restriction endonuclease RI, followed by hybridization to a 420-bp internal fragment of the 16S rRNA gene. Thirty-two type and reference strains were classified, together with seven field strains. Reference strains of all serotypes and the type strains of and were included. Nine ribopatterns were observed. Pattern A was represented by 16 strains and included strains of serotypes 1b, 2 to 8, 11 to 13, 15 to 17, 19, and 23. Pattern B was represented by two strains (serotypes la and 9). Pattern C was represented by five strains (serotypes 5, 6, and 21). Pattern D was represented by one strain of serotype 4. Pattern E was represented by 11 strains of serotypes 2, 7, 10, 20, 22, 24, and 25. Patterns F, G, H, and I were each represented by a single strain of serotypes 26,2,18, and 3, respectively. All the different ribopatterns had some bands in common. Patterns B, C, and D were most similar to pattern A, while patterns F, G, H, and I resembled pattern E. Partial sequencing of the 16S rRNA gene of nine selected strains resulted in three different sequences, i.e., the typical sequence, the sequence, and a third sequence found for two strains. Strains of the same serotype were found to have different ribopatterns as well as different partial 16S rDNA sequences.

  • Published Online:
Copyright © 1995, International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-2-382
1995-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/2/ijs-45-2-382.html?itemId=/content/journal/ijsem/10.1099/00207713-45-2-382&mimeType=html&fmt=ahah

References

  1. Collins M. D., Rodrigues U., Ash C., Aguirre M., Farrow J. A. E., Martinez-Murcia A., Phillips B. A., Williams A. M., Wallbanks S. 1991; Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett. 77:5–12
     [Google Scholar]
  2. Eamens G. J., Turner M. J., Catt R. E. 1988; Serotypes of Erysipelothrix rhusiopathiae in Australian pigs, small ruminants, poultry, and captive wild birds and animals. Aust. Vet. J. 65:249–252
     [Google Scholar]
  3. Grimont F., Grimont P. A. D. 1992; Identification and typing by rRNA gene restriction patterns,. 15–18 Schindler J. Proceedings of the Conference on Taxonomy and Automated Identification of Bacteria Czech-oslovak Society for Microbiology; Prague:
     [Google Scholar]
  4. Hultman T., Bergh S., Moks T., Uhlen M. 1991; Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. BioTechniques 10:84–93
     [Google Scholar]
  5. Hultman T., Ståhl S., Homes E., Uhlén M. 1989; Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17:4937–4946
     [Google Scholar]
  6. Jaquet C., Aubert S., el Solh N., Roucourt J. 1992; Use of rRNA gene restriction patterns for the identification of Listeria . Syst. Appl. Microbiol. 15:42–46
     [Google Scholar]
  7. Johansson M.-L., Molin G., Pettersson B., Uhlén M., Ahrné S. Classification of Lactobacillus plantarum with special reference to restriction fragment length polymorphisms of the 16S rRNA gene. Submitted for publication
  8. Jones D. 1986; Genus Erysipelothrix. 1245–1249 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
     [Google Scholar]
  9. Lucchini G. M., Altwegg M. 1992; rRNA gene restriction patterns as taxonomic tools for the genus Aeromonas . Int. J. Syst. Bacteriol. 442:384–389
     [Google Scholar]
  10. Nørrung V., Molin G. 1991; A new serotype of Erysipelothrix rhusiopathiae isolated from pig slurry. Acta Vet. Hung. 39:137–138
     [Google Scholar]
  11. Pettersson B., Johansson K.-E., Uhlén M. 1994; Sequence analysis of 16S rRNA from mycoplasmas by direct solid-phase DNA sequencing. Appl. Environ. Microbiol. 60:2456–2461
     [Google Scholar]
  12. Reboli A. C, Farrar W. E. 1989; Erysipelothrix rhusiopathiae: an occupational pathogen. Clin. Microbiol. Rev. 2:354–359
     [Google Scholar]
  13. Rodrigues U. M., Aguirre M., Facklam R. R., Collins M. D. 1991; Specific and intra-specific molecular typing of lactococci based on polymorphism of DNA encoding rRNA. J. Appl. Bacteriol. 71:509–516
     [Google Scholar]
  14. Sneath P. H. A. 1978; Classification of microorganisms. 9/1–9/13 Norris J. R., Richmond M. H. Essays in microbiology Wiley; London:
     [Google Scholar]
  15. Ståhl M., Molin G., Persson A., Ahrné S., Ståhl S. 1990; Restriction endonuclease patterns and multivariate analysis as a classification tool for Lactobacillus spp. Int. J. Syst. Bacteriol. 40:189–193
     [Google Scholar]
  16. Ståhl M., Pettersson B., Uhlén M., Molin G., Ahrné S. 1994; Restriction fragment length polymorphism of Lactobacillus reuteri and Lactobacillus fermentum originating from intestinal mucosa, based on 16S rRNA genes. Syst. Appl. Microbiol. 17:108–115
     [Google Scholar]
  17. Stenström I.-M., Norrung V., Ternström A., Molin G. 1992; Occurrence of different serotypes of Erysipelothrix rhusiopathiae in retail pork and fish. Acta Vet. Scand. 33:169–173
     [Google Scholar]
  18. Takahashi T., Fujisawa T., Benno Y., Tamura Y., Sawada T., Suzuki S., Muramatsu M., Mitsuoka T. 1987; Erysipelothrix tonsillarum sp. nov. isolated from tonsils of apparently healthy pigs. Int. J. Syst. Bacteriol. 37:166–168
     [Google Scholar]
  19. Takahashi T., Fujisawa T., Tamura Y., Suzuki S., Muramatsu M., Sawada T., Benno Y., Mitsuoka T. 1992; DNA relatedness among Erysipelothrix rhusiopathiae strains representing all twenty-three serovars and Erysipelothrix tonsillarum . Int. J. Syst. Bacteriol. 42:469–473
     [Google Scholar]
  20. Tamura Y., Takahashi T., Zarkasie K., Nakamura M., Yoshimura H. 1993; Differentiation of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cell proteins. Int. J. Syst. Bacteriol. 43:111–114
     [Google Scholar]
  21. Wellmann G., Kucsera G., Nørrung V. 1983; Comparative studies on different methods in typing strains of Erysipelothrix rhusiopathiae. Methods and influence of some factors on their results. Zentralbl. Bakteriol. Hyg. Abt. 1 Orig. A 254:42–54
     [Google Scholar]
  22. Williams F., Grimont P. A. D. 1991; DNA fingerprinting of Streptococcus uberis based on polymorphism of DNA encoding rRNA.. Lett. Appl. Microbiol. 12:23–28
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-2-382
Loading
Classification of Erysipelothrix Strains on the Basis of Restriction Fragment Length Polymorphisms
Int J Syst Evol Microbiol 45, 382 (1995); https://doi.org/10.1099/00207713-45-2-382
/content/journal/ijsem/10.1099/00207713-45-2-382
/content/journal/ijsem/10.1099/00207713-45-2-382
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error