1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 45, Issue 2

sp. nov., from the Vespid Wasp Free

Abstract

Spiroplasma strain MQ-4 (T = type strain), which was isolated from the hemolymph of the vespid wasp , was serologically distinct from other spiroplasma species, groups, putative groups, and subgroups. Each strain MQ-4 cell was helical and motile and was surrounded by a single cytoplasmic membrane; there was no evidence of a cell wall. The strain grew well in 1% serum fraction medium, as well as in SM-1, M1D, and SP-4 liquid media, under both aerobic and anaerobic conditions. Strain MQ-4 grew at temperatures ranging from 10 to 41°C but did not grow at 43°C. The strain grew optimally at 37°C with a doubling time of 0.6 h, the shortest doubling time recorded for any spiroplasma. Strain MQ-4 catabolized glucose and arginine but did not hydrolyze urea. The guanine-plus-cytosine content of the DNA was about 27.5 ± 1 mol%. The genome size was 1,480 kbp (940 MDa). Strain MQ-4 (= ATCC 35262) is designated the type strain of a new species, .

  • Published Online:
Copyright © 1995, International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-2-203
1995-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/2/ijs-45-2-203.html?itemId=/content/journal/ijsem/10.1099/00207713-45-2-203&mimeType=html&fmt=ahah

References

  1. Alluotto B. B., Wittler R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
     [Google Scholar]
  2. Barile M. F. 1983; Arginine hydrolysis. Methods Mycoplasmol. 1:345–349
     [Google Scholar]
  3. Carle P., Laigret F., Tully J. G., Bové J. M. 1995; Heterogeneity of genome sizes within the genus Spiroplasma . Int. J. Syst. Bacteriol. 45:178–181
     [Google Scholar]
  4. Carle P., Saillard C., Bové J. M. 1983; DNA extraction and purification. Methods Mycoplasmol. 1:295–299
     [Google Scholar]
  5. Carle P., Saillard C., Bové J. M. 1983; Determination of guanine plus cytosine content of DNA. Methods Mycoplasmol. 1:301–308
     [Google Scholar]
  6. Clark T. B. 1982; Spiroplasmas: diversity of arthropod reservoirs and host-parasite relationships. Science 217:57–59
     [Google Scholar]
  7. Clark T. B., Whitcomb R. F., Tully J. G., Mouches C., Saillard C., Bové J. M., Wróblewski H., Carle P., Rose D. L., Henegar R. B., Williamson D. L. 1985; Spiroplasma melliferum, a new species from the honeybee (Apis mellifera). Int. J. Syst. Bacteriol. 35:296–308
     [Google Scholar]
  8. Clyde W. A. Jr. 1983; Growth inhibition tests. Methods Mycoplasmol. 1:405–410
     [Google Scholar]
  9. Freundt E. A. 1983; Film and spot production. Methods Mycoplasmol. 1:373–374
     [Google Scholar]
  10. Gardella R. S., DelGiudice R. A. 1983; Hemagglutination, hemadsorption, and hemolysis. Methods Mycoplasmol. 1:379–384
     [Google Scholar]
  11. Guo Y. H., Chen T. A., Whitcomb R. F., Rose D. L., Tully J. G., Williamson D. L., Ye X. D., Chen Y. X. 1990; Spiroplasma chinense sp. nov. from flowers of Calystegia hederacea in China. Int. J. Syst. Bacteriol. 40:421–425
     [Google Scholar]
  12. Hackett K. J., Clark T. B. 1989; Ecology of spiroplasmas. Mycoplasmas 5:113–200
     [Google Scholar]
  13. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes. 1979; Proposal of minimal standards for descriptions of new species of the class Mollicutes . Int. J. Syst. Bacteriol. 29172–180
     [Google Scholar]
  14. Konai M., Clark E. A., Whitcomb R. F. 1992; Temperature ranges and optima of spiroplasmas. IOM Lett. 2:137
     [Google Scholar]
  15. Mouches C., Bové J. M., Albisetti J., Clark T. B., Tully J. G. 1982; A spiroplasma of serogroup IV causes a May-disease-like disorder of honeybees in southwestern France. Microb. Ecol. 8:387–399
     [Google Scholar]
  16. Razin S. 1983; Urea hydrolysis. Methods Mycoplasmol. 1:351–353
     [Google Scholar]
  17. Razin S., Cirillo V. P. 1983; Sugar fermentation. Methods Mycoplasmol. 1:337–343
     [Google Scholar]
  18. Rose D. L., Tully J. G., Bové J. M., Whitcomb R. F. 1993; A test for measuring growth responses of mollicutes to serum and polyoxyethylene sorbitan. Int. J. Syst. Bacteriol. 43:527–532
     [Google Scholar]
  19. Skripal I. G. 1983; Revival of the name Spiroplasmataceae fam. nov., nom. rev., omitted from the 1980 Approved Lists of Bacterial Names. Int. J. Syst. Bacteriol. 33:408
     [Google Scholar]
  20. Taylor-Robinson D. 1983; Metabolism inhibition tests. Methods Mycoplasmol. 1:411–417
     [Google Scholar]
  21. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol. 1:173–177
     [Google Scholar]
  22. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol. 1:355–362
     [Google Scholar]
  23. Tully J. G., Rose D. L., Clark E., Carle P., Bové J. M., Henegar R. B., Whitcomb R. F., Colflesh D. E., Williamson D. L. 1987; Revised group classification of the genus Spiroplasma (class Mollicutes), with proposed new groups XII to XXIII. Int. J. Syst. Bacteriol. 37:357–364
     [Google Scholar]
  24. Tully J. G., Whitcomb R. F. 1991; The genus Spiroplasma,. 1960–1980 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K. H. The prokaryotes,, 2. 2 Springer-Verlag; New York:
     [Google Scholar]
  25. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol. 1:147–158
     [Google Scholar]
  26. Whitcomb R. F., Bové J. M., Chen T. A., Tully J. G., Williamson D. L. 1987; Proposed criteria for an interim serogroup classification for members of the genus Spiroplasma (class Mollicutes). Int. J. Syst. Bacteriol. 37:82–84
     [Google Scholar]
  27. Whitcomb R. F., Tully J. G., McCawley P., Rose D. L. 1982; Application of the growth inhibition test to Spiroplasma taxonomy. Int. J. Syst. Bacteriol. 32:387–394
     [Google Scholar]
  28. Whitcomb R. F., Tully J. G., Rose D. L., Carle P., Bové J. M., Henegar R. B., Hackett K. J., Clark T. B., Konai M., Adams J., Williamson D. L. 1993; Spiroplasma monobiae sp. nov. from the vespid wasp Monobia quadridens (Hymenoptera: Vespidae). Int. J. Syst. Bacteriol. 43:256–260
     [Google Scholar]
  29. Whitcomb R. F., Tully J. G., Williamson D. L., Bové J. M., French F. E., Konai M., Gasparich G., Abalain-Colloc M. L., Saillard C., Chastel C., Carle P., Rose D. L., Henegar R. B., Clark E. A., Hackett K. J. 1992; Revised classification of spiroplasmas. IOM Lett. 2:135
     [Google Scholar]
  30. Williamson D. L. 1983; Specialized electron microscopic techniques for spiroplasmas in plant and insect tissues. Methods Mycoplasmol. 1:71–76
     [Google Scholar]
  31. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma . Mycoplasmas 5:71–111
     [Google Scholar]
  32. Williamson D. L., Whitcomb R. F. 1983; Special serological tests for spiroplasma identification. Methods Mycoplasmol. 2:249–259
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-2-203
Loading
Spiroplasma velocicrescens sp. nov., from the Vespid Wasp Monobia quadridens
Int J Syst Evol Microbiol 45, 203 (1995); https://doi.org/10.1099/00207713-45-2-203
/content/journal/ijsem/10.1099/00207713-45-2-203
/content/journal/ijsem/10.1099/00207713-45-2-203
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error