1887

Abstract

A spirochete which infects short-tailed shrews () and white-footed mice () has been shown previously to be ultrastructurally and serologically distinct from other spirochetes. Two of the original isolates from Connecticut and Minnesota and 16 new isolates obtained from shrews captured in Minnesota were characterized phenotypically and genetically in this study. A comparative analysis of the 16S rRNA sequences of two shrew isolates and one mouse isolate and the 16S rRNA sequences of 16 other spirochetes and revealed that these organisms exhibited low levels of similarity (range of similarity values, 73.9 to 77.8%; average level of similarity, 74.7%). The shrew and mouse isolates which we examined formed a deeply branching subgroup that was clearly distinct from the other genera of spirochetes examined. These and other results indicated that the new spirochetes represent a unique taxon in the order . Accordingly, we propose that they should be classified as members of a new genus, . The three strains of which we examined had 16S rRNA sequences that were nearly identical. We also compared these isolates by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, fatty acid and enzyme analyses, restriction endonuclease analysis, and Southern hybridization and found that the levels of genetic and phenotypic homogeneity among the strains were very high. We concluded that the isolates which we examined were members of a single species, for which we propose the name . The type strain of is CT11616 (= ATCC 43811). The guanine-plus-cytosine content of the DNA of this species was determined to be 34 to 36 mol% by the thermal denaturation method.

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1995-01-01
2022-05-16
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References

  1. Anderson J. F., Johnson R. C., Magnarelli L. A., Hyde F. W., Andreadis T. G. 1987; New infectious spirochete isolated from short-tailed shrews and white-footed mice. J. Clin. Microbiol. 25:1490–1494
    [Google Scholar]
  2. Anderson J. F., Magnarelli L. A. 1992; Epizootiology of Lyme disease and methods of cultivating Borrelia burgdorferi . Ann. N.Y. Acad. Sci. 653:52–63
    [Google Scholar]
  3. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136:175–179
    [Google Scholar]
  4. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc. Natl. Acad. SciUSA 75:4801–4805
    [Google Scholar]
  5. Defosse D. L., Paster B. J., Dewhirst F. E., Fraser G. J., Johnson R. C. 1993; Brevinema andersonii gen. nov., sp. nov., a new infectious spirochete isolated from feral shrews and mice, abstr. R-12. 295In Abstracts of the 93rd General Meeting of the American Society for Microbiology 1993 American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  6. Dewhirst F. E., Paster B. J., Bright P. L. 1989; Chromobacterium, Eikenella, Kingella, Neiserria, Simonsiella and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Neisseriaceae (emend.). Int. J. Syst. Bacteriol. 39:258–266
    [Google Scholar]
  7. Guilian G. G., Moss R. L., Greaser M. 1983; Improved methodology for analysis and quantitation of proteins on one-dimensional silver stained slab gels. Anal. Biochem. 129:277–287
    [Google Scholar]
  8. Hughes C. A. N., Kodner C. B., Johnson R. C. 1992; DNA analysis of NCH-1, the first north central U.S. human Lyme disease isolate. J. Clin. Microbiol. 30:698–703
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 Munro H. N. Mammalian protein metabolism 3 Academic Press, Inc.; New York:
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 1993; MEGA: molecular evolutionary genetic analysis, version 1.0. The Pennsylvania State University; University Park:
    [Google Scholar]
  11. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. SciUSA 82:6955–6959
    [Google Scholar]
  12. LeFebvre R. B., Perng G. C. 1989; Genetic and antigenic characterization of Borrelia coriaceae putative agent of epizootic bovine abortion. J. Clin. Microbiol. 27:389–393
    [Google Scholar]
  13. LeFebvre R. B., Perng G. C., Johnson R. C. 1989; Characterization of Borrelia burgdorfen isolates by restriction endonuclease analysis and DNA hybridization. J. Clin. Microbiol. 27:636–639
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  15. Pace B., Matthews E. A., Johnson K. D., Cantor C. R., Pace N. R. 1982; Conserved 5S rRNA complement to tRNA is not required for protein synthesis. Proc. Natl. Acad. SciUSA 79:36–40
    [Google Scholar]
  16. Paster B. J., Dewhirst F. E. 1988; Phylogeny of campylobacters, wolinellas, Bacteroides gracilis and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol. 38:56–62
    [Google Scholar]
  17. Paster B. J., Dewhirst F. E., Weisberg W. G., Tardoff L. A., Fraser G. J., Hespell R. B., Stanton T. B., Zablen L., Mandelco L., Woese C. R. 1991; Phylogenetic analysis of the spirochetes. J. Bacteriol. 173:6101–6109
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  19. Sasser M., Wichman M. D. 1991; Identification of microorganisms through the use of gas chromatography and high-performance liquid chromatography. 111–118 Hausler W. J. Jr, Herrmann K. L., Isenberg H. D., Shadomy H. D. Manual of clinical microbiology, 5. American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  20. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. SciUSA 76:4350–4354
    [Google Scholar]
  21. Weisburg W. G., Hatch R. P., Woese C. R. 1986; Eubacterial origin of the chlamydiae. J. Bacteriol. 167:570–574
    [Google Scholar]
  22. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
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