1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 45, Issue 1

gen. nov., a New Genus of the Order , and Description of sp. nov. and sp. nov. Free

Abstract

Ten strains of thermophilic actinomycetes were isolated from waste and mushroom composts, as well as from the air of compost plants and a refuse incineration plant in Germany. These organisms produce white aerial mycelia and form hyphae with so-called pseudosporangia that fragment into rod-like structures. The organisms have type III cell walls (-diaminopimelic acid and whole-cell sugar type C), the phospholipid type is type PII, and mycolic acids are not present. The major menaquinone is MK-9(H), and the fatty acids are mainly iso- and anteiso-branched fatty acids, hydroxy fatty acids, and 10-methyl-branched fatty acids. The guanine-plus-cytosine content of the DNA is 69 to 73 mol%. The chemotaxonomic markers (especially whole-cell sugar type C) and 16S ribosomal DNA sequence data indicated that these organisms represent a new genus of the order , for which the name is proposed. On the basis of phylogenetic and phenotypic data, this new genus is closely related to members of the family and related taxa and contains two species: sp. nov. and sp. nov. The type species of the genus is , with type strain MKD 35 (= DSM 44069), and the type strain of is CHB 77 (= DSM 44070).

  • Published Online:
Copyright © 1995 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-1-67
1995-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/1/ijs-45-1-67.html?itemId=/content/journal/ijsem/10.1099/00207713-45-1-67&mimeType=html&fmt=ahah

References

  1. Amner W., Edwards C., McCarthy A. J. 1989; Improved medium for recovery and enumeration of the farmer's lung organism, Saccharomono-spora viridis . Appl. Environ. Microbiol. 55:2669–2674
     [Google Scholar]
  2. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes. Appl. Microbiol. 13:236–243
     [Google Scholar]
  3. Becking J. H. 1961; Studies on nitrogen-fixing bacteria of the genus Beijerinckia. II. Mineral nutrition and resistance to high levels of certain elements in relation to soil type. Plant Soil 14:297–322
     [Google Scholar]
  4. Bowen T., Stackebrandt E., Dorsch M., Embley T. M. 1989; The phylogeny of Amycolata autotrophica, Kibdelosporangium aridum and Sac-charothrix australiensis . J. Gen. Microbiol. 135:2529–2536
     [Google Scholar]
  5. Brosius J., Palmer J. J., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc. Natl. Acad. SciUSA 75:4801–805
     [Google Scholar]
  6. Collins M. D., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J. Gen. Microbiol. 100:221–230
     [Google Scholar]
  7. Cross T., Attwell R. 1974; Recovery of viable thermoactinomycete endospores from deep mud cores. 11–20 Barker A. N., Gould G. W., Wolf J. Spore research 1973 Academic Press; London:
     [Google Scholar]
  8. Cross T., Lechevalier M. P., Lechevalier H. 1963; A new genus of the Actinomycetales: Microellobosporia gen. nov. J. Gen. Microbiol. 31:421–429
     [Google Scholar]
  9. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
     [Google Scholar]
  10. El-Nakeeb M. A., Lechevalier H. A. 1963; Selective isolation of aerobic actinomycetes. Appl. Microbiol. 11:75–77
     [Google Scholar]
  11. Embley T. M. 1992; The family Pseudonocardiaceae . 996–1027 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes Springer Verlag; Berlin:
     [Google Scholar]
  12. Fergus C. L. 1964; Thermophilic and thermotolerant molds and actinomycetes of mushroom compost during peak heating. Mycologia 56:267–284
     [Google Scholar]
  13. Gochnauer M. B., Leppard G. G., Komaratat P., Kates M., Novitsky T., Kushner D. J. 1975; Isolation and characterization of Actinopolyspora halophila gen. et sp. nov., an extremely halophilic actinomycete. Can. J. Microbiol. 21:1500–1511
     [Google Scholar]
  14. Goodfellow M., Lacey J., Athalye M., Embley T. M., Bowen T. 1989; Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J. Gen. Microbiol. 135:2125–2139
     [Google Scholar]
  15. Greiner-Mai E., Korn-Wendisch F., Kutzner H. J. 1988; Taxonomic revision of the genus Saccharomonospora and description of Saccharomono-spora glauca sp. nov. Int. J. Syst. Bacteriol. 38:398–405
     [Google Scholar]
  16. Greiner-Mai E., Kroppenstedt R. M., Korn-Wendisch F., Kutzner H. J. 1987; Morphological and biochemical characterization and emended description of thermophilic actinomycetes species. Syst. Appl. Microbiol. 9:97–106
     [Google Scholar]
  17. Harrigan W. F., McCance M. E. 1966; Laboratory methods in microbiology. Academic Press, Inc. (London), Ltd.; London:
     [Google Scholar]
  18. Hasegawa T. 1988; Actinokineospora: a new genus of the Actinomycetales . Actinomycetology 2:31–45
     [Google Scholar]
  19. Held T., Kutzner H. J. 1990; Transcription of the tyrosinase gene in Streptomyces michiganensis DSM 40015 is induced by copper and repressed by ammonium. J. Gen. Microbiol. 136:2413–2419
     [Google Scholar]
  20. Hsu S. C, Lockwood J. L. 1975; Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl. Microbiol. 29:422–426
     [Google Scholar]
  21. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 Munro H. N. Mammalian protein metabolism Academic Press, Inc.; New York:
     [Google Scholar]
  22. Kempf A., Greiner-Mai E., Schneider J., Korn-Wendisch F, Kutzner H. J. 1987; A group of actinophages of Faenia rectivirgula . Curr. Microbiol. 15:283–285
     [Google Scholar]
  23. Kempf A., Kutzner H. J. 1989; Screening von biopolymerabbauenden Exoenzymen bei thermophilen Actinomyceten. 979–989 VDLUFA (Verband Deutscher Landw. Untersuchungs- und Forschungsanstalten) Schriftenreihe 28, Kongressband 1988 VDLUFA-Verlag; Darmstadt, Germany:
     [Google Scholar]
  24. Korn F., Weingärtner B., Kutzner H. J. 1978; A study of twenty actinophages: morphology, serological relationship and host range. 251–270 Freerksen E., Tarnok I., Thumin J. H. Genetics of the Actinomycetales Gustav Fischer Verlag; Stuttgart, Germany:
     [Google Scholar]
  25. Korn-Wendisch F., Kempf A., Grand E., Kroppenstedt R. M., Kutzner H. J. 1989; Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. tuberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora . Int. J. Syst. Bacteriol. 39:430–441
     [Google Scholar]
  26. Korn-Wendisch F., Kutzner H. J. 1992; The family Streptomycetaceae . 921–995 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes Springer Verlag; Berlin:
     [Google Scholar]
  27. Korn-Wendisch F., Schneider J. 1992; Phage typing—a useful tool in actinomycete systematics. Gene 115:243–247
     [Google Scholar]
  28. Kothe H. W., Vobis G., Kroppenstedt R. M., Henssen A. 1989; A taxonomic study of mycolateless wall chemotype IV actinomycetes. Syst. Appl. Microbiol. 12:61–69
     [Google Scholar]
  29. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reversed phase (RP18) and silver loaded ion exchanger as stationary phases. J. Liq. Chromatogr. 5:2359–2367
     [Google Scholar]
  30. Kroppenstedt R. M. 1985; Fatty acid analysis and menaquinone analysis of actinomycetes and related organisms. Soc. Appl. Bacteriol. Tech. Ser. 20:173–199
     [Google Scholar]
  31. Kroppenstedt R. M. 1992; The genus Nocardiopsis . 1139–1156 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes Springer Verlag; Berlin:
     [Google Scholar]
  32. Kroppenstedt R. M., Goodfellow M. 1992; The family Thermomono-sporaceae . 1085–1114 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. The prokaryotes Springer Verlag; Berlin:
     [Google Scholar]
  33. Kurup V. P. 1984; Thermophilic actinomycetes: their role in hypersensitivity pneumonitis. 145–159 Ortiz-Ortiz L., Bojajil L. F., Yakoleff V. Biological, biochemical, and biomedical aspects of actinomycetes Academic Press, Inc. (London), Ltd.; London:
     [Google Scholar]
  34. Kutzner H. J. 1981; The family Streptomycetaceae . 2028–2090 Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. The prokaryotes Springer Verlag; Berlin:
     [Google Scholar]
  35. Kutzner H. J., Böttiger V., Heitzer R. D. 1978; The use of physiological criteria in the taxonomy of Streptomyces and Streptoverticillium . 25–29 Mordarski M., Kurylowicz W., Jeljaszewicz J. Nocardia and StreptomycesProceedings of the International Symposium on Nocardia and Streptomyces, Warsaw, 1976Gustav Fischer Verlag, Stuttgart, Germany
     [Google Scholar]
  36. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. 1984; Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis . Int. J. Syst. Bacteriol. 34:426–431
     [Google Scholar]
  37. Lacey J. 1988; Actinomycetes as biodeteriogens and pollutants of the environment. 359–432 Goodfellow M., Williams S. T., Mordarski M. Actinomycetes in biotechnology Academic Press; San Diego, Calif:
     [Google Scholar]
  38. Larsen N., Olsen G. J., Maidak B. L., McCaughey M. J, Overbeek R., Macke T. J., Marsh T. L., Woese C. R. 1993; The Ribosomal Database Project. Nucleic Acids Res. 21:3021–3023
     [Google Scholar]
  39. Lechevalier H. A., Lechevalier M. P., Gerber N. N. 1971; Chemical composition as a criterion in the classification of actinomycetes. Adv. Appl. Microbiol. 14:47–72
     [Google Scholar]
  40. Lechevalier M. P., de Bievre D., Lechevalier H. A. 1977; Chemotax-onomy of aerobic actinomycetes: phospholipid composition. Biochem. Syst. Ecol. 5:249–260
     [Google Scholar]
  41. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int. J. Syst. Bacteriol. 20:435–444
     [Google Scholar]
  42. Lechevalier M. P., Prauser H., Labeda D. P., Ruan J.-S. 1986; Two new genera of nocardioform actinomycetes: Amycolata gen. nov. and Amycola-topsis gen. nov. Int. J. Syst. Bacteriol. 36:29–37
     [Google Scholar]
  43. Meyer J. 1989; Genus Actinomadura Lechevalier and Lechevalier 1970a, 400AL . 2511–2526 Williams S. T., Sharpe M. E., Holt J. G. Bergey's manual of systematic bacteriology 4 Williams & Wilkins; Baltimore:
     [Google Scholar]
  44. Meyer J. 1989; Genus Nocardiopsis Meyer 1976, 487AL . 2562–2568 Williams S. T., Sharpe M. E., Holt J. G. Bergey's manual of systematic bacteriology 4 Williams & Wilkins; Baltimore:
     [Google Scholar]
  45. Miller L. T. 1982; A single derivatization method for bacterial fatty acid methyl esters including hydroxy acids. J. Clin. Microbiol. 16:584–586
     [Google Scholar]
  46. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia, and related taxa by thin layer Chromatographie analysis of whole-cell methanolysates. J. Gen. Microbiol. 88:200–204
     [Google Scholar]
  47. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2:233–241
     [Google Scholar]
  48. Nonomura H. 1989; Genus Microtetraspora Thiemann, Pagani and Beretta 1968b, 296AL . 2531–2536 Williams S. T., Sharpe M. E., Holt J. G. Bergey's manual of systematic bacteriology 4 Williams & Wilkins; Baltimore:
     [Google Scholar]
  49. Owen R. J., Pitcher D. 1985; Current methods for estimating base composition and levels of DNA-DNA hybridization. Soc. Appl. Bacteriol. Tech. Ser. 20:67–93
     [Google Scholar]
  50. Rainey F. A. R., Dorsch M., Morgan H. W., Stackebrandt E. 1992; 16S rDNA analysis of Spirochaeta thermophilia: its phylogenetic position and implications for the systematics of the order Spirochaetales . Syst. Appl. Microbiol. 15:197–202
     [Google Scholar]
  51. Rainey F. A. R., Stackebrandt E. 1993; Phylogenetic analysis of the bacterial genus Thermobacteroides indicates an ancient origin of Thermobac-teroides proteolyticus . Lett. Appl. Microbiol. 16:282–286
     [Google Scholar]
  52. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol. 49:1–7
     [Google Scholar]
  53. Schneider J., Korn-Wendisch F., Kutzner H. J. 1990; ϕSC623, a temperate actinophage of Streptomyces coelicolor Müller, and its relatives ϕSC347 and ϕSC681. J. Gen. Microbiol. 136:767–772
     [Google Scholar]
  54. Schneider J., Kramer D., Grund E., Kutzner H. J. 1989; Preliminary characterization of a group of actinophages of the thermophilic actinomycete genus Saccharomonospora . Intervirology 30:323–329
     [Google Scholar]
  55. Schneider J., Kutzner H. J. 1989; Distribution of modules among the central regions of the genomes of several actinophages of Faenia and Saccharopolyspora . J. Gen. Microbiol. 135:1671–1678
     [Google Scholar]
  56. Schneider J., Kutzner H. J. 1991; Description of eight new actinophage species of the Pseudonocardiaceae genus Saccharopolyspora . Syst. Appl. Microbiol. 14:72–78
     [Google Scholar]
  57. Shearer M. C, Colman P. M., Ferrin R. M., Nisbet L. J., Nash C. H. 1986; A new genus of the Actinomycetales: Kibdelosporangium aridum gen. nov., sp. nov. Int. J. Syst. Bacteriol. 36:47–54
     [Google Scholar]
  58. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int. J. Syst. Bacteriol. 16:313–340
     [Google Scholar]
  59. Stackebrandt E., Kroppenstedt R. M., Jahnke K.-D., Kemmerling C., Gürtler H. 1994; Transfer of Streptosporangium viridogriseum (Okuda et al. 1966), Streptosporangium viridogriseum subsp. kofuense (Nonomura and Ohara 1969), and Streptosporangium albidum (Furumai et al. 1968) to Kutzneria gen. nov. as Kutzneria viridogrisea comb, nov., Kutzneria kofuensis comb, nov., and Kutzneria albida comb, nov., respectively, and emendation of the genus Streptosporangium . Int. J. Syst. Bacteriol. 44:265–269
     [Google Scholar]
  60. Stanek J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28:226–231
     [Google Scholar]
  61. Takeuchi M., Nishii T., Yokota A. 1992; Taxonomic significance of arabinosè in the family Pseudonocardiaceae . Actinomycetology 6:79–90
     [Google Scholar]
  62. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
     [Google Scholar]
  63. Van den Bogart H. G. G., Van den Ende G., Van Loon P. C. C., Van Griensven L. J. L. D. 1993; Mushroom worker's lung: serologic reactions to thermophilic actinomycetes present in the air of compost tunnels. Mycopathologia 122:21–28
     [Google Scholar]
  64. Warwick S., Bowen T., McVeigh H, Embley T. M. 1994; A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the geneiaAmycolata and Pseudonocardia in an emended genus Pseudonocardia . Int. J. Syst. Bacteriol. 44:293–299
     [Google Scholar]
  65. Williams S. T., Cross T. 1971; Actinomycetes. Methods Microbiol. 4:295–334
     [Google Scholar]
  66. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . 2452–2492 Williams S. T., Sharpe M. E., Holt J. G. Bergey's manual of systematic bacteriology 4 Williams & Wilkins; Baltimore:
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-1-67
Loading
Thermocrispum gen. nov., a New Genus of the Order Actinomycetales, and Description of Thermocrispum municipale sp. nov. and Thermocrispum agreste sp. nov.
Int J Syst Evol Microbiol 45, 67 (1995); https://doi.org/10.1099/00207713-45-1-67
/content/journal/ijsem/10.1099/00207713-45-1-67
/content/journal/ijsem/10.1099/00207713-45-1-67
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error