1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 45, Issue 1

gen. nov., sp. nov., a New Gram-Positive Polyphosphate-Accumulating Bacterium Isolated from Activated Sludge Free

Abstract

Polyphosphate-accumulating bacteria that were previously isolated from activated sludge and exhibited high phosphate removal activity were studied taxonomically and phylogenetically. These organisms were gram-positive, coccus-shaped, aerobic chemoorganotrophs that had a strictly respiratory type of metabolism in which oxygen was a terminal electron acceptor. They accumulated large amounts of polyphosphate under aerobic conditions. The major quinone was menaquinone MK-9(H). The cell wall peptidoglycan contained -diaminopimelic acid. The guanine-plus-cytosine content of the DNA was 67.9 mol%. Our isolates were similar phenotypically and chemotaxonomically to , which was proposed recently as a new genus and species. However, our isolates differed from in cellular fatty acid composition and some other traits. A phylogenetic analysis based on 16S rRNA sequences showed that our isolate differ from the genus and other genera belonging to the high-G+C-content gram-positive group. Accordingly, we concluded that our strain NM-1 (T = type strain) should be assigned to a new genus and species, for which we propose the name .

  • Published Online:
Copyright © 1995 International Union of Microbiological Societies
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-45-1-17
1995-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/45/1/ijs-45-1-17.html?itemId=/content/journal/ijsem/10.1099/00207713-45-1-17&mimeType=html&fmt=ahah

References

  1. Bergan T., Kocur M. 1982; Stomatococcus mucilaginosus gen. nov., sp. rev., a member of the family Micrococcaceae . Int. J. Syst. Bacteriol 32:374–377
     [Google Scholar]
  2. Brooks B. W., Murray. R. G. E. 1981; Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int. J. Syst. Bacteriol 31:353–360
     [Google Scholar]
  3. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc. Natl. Acad. SciUSA 75:4801–4805
     [Google Scholar]
  4. Callaway J. O. 1989; Determination of inorganic nonmetalic constituents. 4-175–4-176 Clesceli L. S. Standard methods for the examination of water and wastewater, 17. American Public Health Association; Washington, D.C:
     [Google Scholar]
  5. Collins M. D., Stackebrandt E. 1989; Molecular taxonomic studies on some ll-diaminopimelic acid-containing coryneforms from herbage: description of Nocardioides fastidiosa sp. nov. FEMS Microbiol. Lett 57:289–294
     [Google Scholar]
  6. Dainema M. H., van Loosdrecht M., Schölten A. 1985; Some physiological characteristics of Acinetobacter spp. accumulating large amounts of phosphate. Water Sci. Technol 17:119–125
     [Google Scholar]
  7. Fuhs C. W., Chen M. 1975; Microbial basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microb. Ecol 2:119–138
     [Google Scholar]
  8. Higgines D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comput. Appl. Biosci 8:189–191
     [Google Scholar]
  9. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol 15:210–213
     [Google Scholar]
  10. Hiraishi A., Shin Y. K., Sugiyama J. 1992; Rapid profiling of bacterial quinones by two-dimensional thin-layer chromatography. Lett. Appl. Microbiol 14:170–173
     [Google Scholar]
  11. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J. 1994; Automated sequencing of PCR-amplified 16S rDNA on ‘Hydrolink’ gels. J. Microbiol. Methods 19:145–154
     [Google Scholar]
  12. Kamagata Y., Mikami E. 1991; Isolation and characterization of a novel thermophilic Methanosaeta strain. Int. J. Syst. Bacteriol 41:191–196
     [Google Scholar]
  13. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol 16:111–120
     [Google Scholar]
  14. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
     [Google Scholar]
  15. Lowson E. N., Tonhazy N. E. 1980; Changes in morphology and phosphate uptake patterns of Acinetobacter calcoaceticus strains. Water S A (Pretoria) 6:105–112
     [Google Scholar]
  16. Magee C. M., Rodeheaver G., Edgerton R. F. 1975; A more reliable Gram staining technique for diagnosis of surgical infections. Am. J. Surg 130:341–346
     [Google Scholar]
  17. Miller E. S., Woese C. R., Brenner S. 1991; Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium gen. nov., sp. nov. Int. J. Syst. Bacteriol 41:363–368
     [Google Scholar]
  18. Nakamura K., Masuda K., Mikami E. 1989; Polyphosphate accumulating bacteria and their ecological characteristics in activated sludge process. 427–431 Hattori T. Recent advances in microbial ecology Japan Scientific Societies Press; Tokyo:
     [Google Scholar]
  19. Nakamura K., Masuda K., Mikami E. 1991; Isolation of a new type of polyphosphate accumulating bacterium and its phosphate removal characteristics. J. Ferment. Technol 4:258–263
     [Google Scholar]
  20. Pitcher D. G., Collins M. D. 1991; Phylogenetic analysis of some ll-diaminopimelic acid-containing coryneform bacteria from human skin: description of Propionibacterium innocuum sp. nov. FEMS Microbiol. Lett 84:295–300
     [Google Scholar]
  21. Poston J. M. 1976; Pelczaria aurantia gen. nov., sp. nov., a newly described orange-colored bacterium. Arch. Microbiol 160:114–120
     [Google Scholar]
  22. Prauser H. 1976; Nocardioides, a new genus of the order Actinomycetales . Int. J. Syst. Bacteriol 26:58–65
     [Google Scholar]
  23. Satiou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
     [Google Scholar]
  24. Schleifer K. H. 1986; Section 12. Gram-positive cocci. 999–1103 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Bergey’s manual of systematic bacteriology 4 The Williams & Wilkins Co; Baltimore:
     [Google Scholar]
  25. Shoda M., Ohsumi T., Udaka S. 1980; Screening for high phosphate accumulating bacteria. Agric. Biol. Chem 44:319–324
     [Google Scholar]
  26. Stackebrandt E., Woese C. R. 1979; A phylogenetic dissection of the family Micrococcaceae . Curr. Microbiol 2:317–322
     [Google Scholar]
  27. Suresh N., Warburg R., Timmerman M., Wells J., Coccia M., Roberts M. F., Halvorson H. O. 1985; New strategies for the isolation of microorganisms responsible for phosphate accumulation. Water Sci. Technol 17:11/1243–56
     [Google Scholar]
  28. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett 25:125–128
     [Google Scholar]
  29. Tamura T., Takeuchi M., Yokota A. 1994; Luteococcus japonicus gen. nov., sp. nov., a new gram-positive coccus with LL-diaminopimelic acid in the cell wall. Int. J. Syst. Bacteriol 44:348–356
     [Google Scholar]
  30. Wagner M., Erhart R., Manz W., Amann R., Lemmer H., Wedi D., Schleifer K.-H. 1994; Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol 60:792–800
     [Google Scholar]
  31. Yokota A., Tamura T., Nishii T., Hasegawa T. 1993; Kineococcus aurantiacus gen. nov., sp. nov., a new aerobic, gram-positive, motile coccus with meso-diaminopimelic acid and arabinogalactan in the cell wall. Int. J. Syst. Bacteriol 43:52–57
     [Google Scholar]
  32. Yokota A., Tamura T., Takeuchi M., Weiss N., Stackebrandt E. 1994; Transfer of Propionibacterium innocuum Pitcher and Collins 1991 to Propioniferax gen. nov. as Propioniferax innocuum comb. nov. Int. J. Syst. Bacteriol 44:579–582
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-45-1-17
Loading
Microlunatus phosphovorus gen. nov., sp. nov., a New Gram-Positive Polyphosphate-Accumulating Bacterium Isolated from Activated Sludge
Int J Syst Evol Microbiol 45, 17 (1995); https://doi.org/10.1099/00207713-45-1-17
/content/journal/ijsem/10.1099/00207713-45-1-17
/content/journal/ijsem/10.1099/00207713-45-1-17
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error