Genomic Diversity and Phylogenetic Relationships among Lipid-Requiring Diphtheroids from Humans and Characterization of Corynebacterium macginleyi sp. nov.
* Corresponding author. Mailing address: Institut de Bactériologie de la Faculté de Médecine, 3 rue Koeberlé, F-67000 Strasbourg, France. Phone: 33.88.21.19.70. Fax: 33.88.25.11.13.
DNA relatedness experiments were performed with 38 clinical isolates and 13 reference strains of coryneform taxa exhibiting a lipid requirement for optimal growth. Forty-five of these strains split into five genomic groups at the species level, whereas six other strains remained unclustered. Genomospecies II fits Corynebacterium accolens, but the other genomospecies were not genetically related to any of the defined Corynebacterium species. Phylogenetic analyses of genes coding for small-subunit rRNA sequences revealed that two genomospecies (I and III) and C. accolens form a tight cluster within the robust branch that groups all Corynebacterium species presently sequenced. Reference strains of biotypes C-1, C-2, and C-3 of “Corynebacterium pseudogenitalium” were found to fall into genomospecies I, as well as “Corynebacterium tuberculostearicum” center for Disease Control and Prevention (CDC) coryneform group G-1, and CDC coryneform group G-2 reference strains. Biochemical tests allowed differentiation between genomospecies except between genomospecies IV and V and between six unclustered strains and genomospecies I. We propose a new classification for these lipid-requiring diphtheroids within the genus Corynebacterium with the delineation of some CDC coryneform group G-1 strains (genomospecies III) as a new species for which the name Corynebacterium macginleyi is proposed. The type strain is strain JCL-2 (CIP 104099), isolated from a human corneal ulcer.
BernardK. A.,
BellefeuilleM.,
EwanE. P.1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J. Clin. Microbiol 29:83–89
BrennerD. J.,
GrimontP. A. D.,
SteigerwaltA. G.,
FanningG. R.,
AgeronE.,
RiddleC. F.1993; Classification of citrobacteria by DNA hybridization: designation of Citrobacter farmed sp. nov., Citrobacteryoungae sp. nov., Citrobacter braakii sp. nov., Citrobacter werkmanni sp. nov., Citrobacter sedlakii sp. nov., and three unnamed Citrobacter genomospecies. Int. J. Syst. Bacteriol 43:645–658
Cocito C,
DelvilleJ.1985; Biological, chemical, immunological, and staining properties of bacteria isolated from tissues of leprosy patients. Eur. J. Epidemiol 1:202–231
De BrielD.,
CoudercF.,
RiegelP.,
JehlF.,
MinckR.1992; High-performance liquid chromatography of corynomycolic acids as a tool in identification of Corynebacterium species and related organisms. J. Clin. Microbiol 30:1407–1417
EvangeiistaA. T.,
SahaA.,
LechevalierM. P.,
FurnessG.1978; Analysis of the cell wall constituents of Corynebacterium genitalium. Int. J. Syst. Bacteriol 28:344–348
FurnessG.,
EvangeiistaA. T.1978; A diagnostic key employing biological reactions for differentiating pathogenic Corynebacterium genitalium (NSU corynebacteria) from commensals of the urogenital tract. Invest. Urol 16:1–4
GrimontP. A. D.,
PopoffM. Y.,
GrimontF.,
CoynaultC.,
LemelinM.1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol 4:325–330
ImaedaT.,
CoppolaK. M.,
FurnessG.1985; Deoxyribonucleic acids of Corynebacterium genitalium and Corynebacterium pseudogenitalium: their genome molecular weights, base ratios, and DNA relatedness with other corynebacteria involved in urinary tract infections. Can. J. Microbiol 31:1068–1070
LeydenJ. J.,
McGinleyK. J.,
HôlzleE.,
LabowsJ. N.,
KligmanA. M.1981; The microbiology of the human axilla and its relationship to axillary odor. J. Invest. Dermatol 77:413–416
McGinleyK. J.,
LabowsJ. N.,
ZechmanJ. M.,
NordstromK. M.,
WebsterG. F.,
LeydenJ. J.1985; Analysis of cellular components, biochemical reactions, and habitat of human cutaneous lipophilic diphtheroids. J. Invest. Dermatol 85:374–377
RiegelP.,
de BrielD.,
PrévostG.,
JehlF.,
MonteilH.1994; Genomic diversity among Corynebacterium jeikeium strains and comparison with biochemical characteristics and antimicrobial susceptibilities. J. Clin. Microbiol 32:1860–1865
RiegelP.,
de BrielD.,
PrévostG.,
JehlF.,
MonteilH.,
MinckR.1993; Taxonomic study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C. afermen-tans subsp. afermentans subsp. nov. and C. afermentans subsp. lipophilum subsp. nov. Int. J. Syst. Bacteriol 43:287–292
RiegelP.,
GrimontP. A. D.,
de BrielD.,
AgeronE.,
JehlF.,
PelegrinM.,
MonteilH.,
MinckR.1992; Corynebacterium group D-2 (“Corynebacterium urealyticum”) constitutes a new genomic species. Res. Microbiol 143:307–313
RuimyR.,
BreittmayerV.,
ElbazeP.,
LafayB.,
BoussemartO.,
GauthierM.,
ChristenR.1994; Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences. Int. J. Syst. Bacteriol 44:416–426
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol 44:846–849
SwoffordD.1990; PAUP: phylogenetic analysis using parsimony, version 3.0. Computer program distributed by the Illinois Natural History Survey. Champaign 111:
Genomic Diversity and Phylogenetic Relationships among Lipid-Requiring Diphtheroids from Humans and Characterization of Corynebacterium macginleyi sp. nov.