The genetic relationships among species were assessed by using DNAs from American Type Culture Collection type strains of 10 species and two subspecies of the genus , i.e., subsp. , and subsp. . The DNA base compositions (determined by high-performance liquid chromatography) of these species varied widely, from 38 to 62 mol% G+C. Seven species showed significant differences (nearly 10%) in G+C content compared with , the type species of the genus. DNA-DNA hybridization (by the membrane filter method) showed that two subspecies, subsp. yurii and subsp. , and two strains of exhibited high levels of DNA relatedness. However, the DNA reassociations among the 10 species studied were 1 to 16%. None of the species examined shared a high level of DNA reassociation with the type species of the genus . The protein profile patterns (determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) of whole bacterial cells from these species were distinct, and no major peptide bands were shared among the 10 species. Therefore, the species we tested must be considered genetically distinct from each other.


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