Several strains of the four sibling species of the genus (, and ) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as I, I, I, and I) made it possible to differentiate each species. and presented a greater number of large fragments than and with all the assay enzymes. With I and I, species-specific bands clearly permitted differentiation between and . To test the resolution of this method, wild strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping.


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