gen. nov., a New Genus of Phototrophic Bacteria Including as comb. nov. and sp. nov. Free

Abstract

Abstract

Two new strains (AS130 and AS140) of phototrophic purple nonsulfur bacteria isolated from activated sludge were characterized and compared with and some other species of the genus The new isolates produced pink photosynthetic cultures, had rod-shaped cells that divided by budding, and formed intracytoplasmic membranes of the lamellar type together with bacteriochlorophyll and carotenoids of the normal spirilloxanthin series. They were also characterized by their capacity for complete denitrification and their production of both ubiquinone-10 and rhodoquinone-10 as major quinones. The isolates were phenotypically most similar to but exhibited low levels of genomic DNA hybridization to this species and to all other species compared. Phylogenetic analyses on the basis of PCR-amplified 16S rRNA gene sequences showed that our isolates and formed a cluster distinct from other members of the genus The phenotypic, genotypic, and phylogenetic data show that the new isolates and should be placed in a new single genus rather than included in the genus Thus, we propose to transfer to a new genus, as gen. nov., comb. nov. (type species) and to designate strains AS130 and AS140 a new species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-4-665
1994-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/44/4/ijs-44-4-665.html?itemId=/content/journal/ijsem/10.1099/00207713-44-4-665&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75:4801–4805
    [Google Scholar]
  2. De Ley J. 1992; The Proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. 2111–2140 In Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. (ed.) The prokaryotes, 2nd ed.. Springer-Verlag; Berlin:
    [Google Scholar]
  3. Drews G., Giesbrecht P. 1966; Rhodopseudomonas viridis, nov. spec, ein neu isoliertes, obligat phototrophes Bakterium. Arch. Mikrobiol. 53:255–262
    [Google Scholar]
  4. Ezaki T., Dejsirilert S., Yamamoto H., Takeuchi N., Liu S., Yabuuchi E. 1988; Simple and rapid genetic identification of Legionella species with photobiotin-labeled DNA. J. Gen. Appl. Microbiol. 34:191–199
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comp. Appl. Biosci. 8:189–191
    [Google Scholar]
  7. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett. Appl. Microbiol. 15:210–213
    [Google Scholar]
  8. Hiraishi A., Hoshino Y. 1984; Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J. Gen. Appl. Microbiol. 30:435–448
    [Google Scholar]
  9. Hiraishi A., Hoshino Y., Satoh T. 1991; Rhodoferax fermentons gen. nov., sp. nov., a phototrophic purple nonsulfur bacterium previously referred to as the “Rhodocyclus gelatinosus-like” group. Arch. Microbiol. 155:330–336
    [Google Scholar]
  10. Hiraishi A., Kitamura H. 1984; Distribution of phototrophic purple nonsulfur bacteria in activated sludge systems and other aquatic environments. Bull. Jpn. Soc. Sci. Fish. 50:1929–1937
    [Google Scholar]
  11. Hiraishi A., Santos T. S., Sugiyama J., Komagata K. 1992; Rhodopseudomonas rutila is a later subjective synonym of Rhodopseudomonas palustris. Int. J. Syst. Bacteriol. 42:186–188
    [Google Scholar]
  12. Hiraishi A., Shi J.-L., Kitamura H. 1989; Effects of organic nutrient strength on the purple nonsulfur bacterial content and metabolic activity of photosynthetic sludge for wastewater treatment. J. Ferment. Bioeng. 68:269–276
    [Google Scholar]
  13. Hiraishi A., Shin Y. K., Ueda Y., Sugiyama J. 1994; Automated sequencing of PCR-amplified 16S rDNA on “Hydrolink” gels. J. Microbiol. Methods 19:145–154
    [Google Scholar]
  14. Hiraishi A., Ueda Y. 1994; Intrageneric structure of the genus Rhodobacter. transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov. Int. J. Syst. Bacteriol. 44:15–23
    [Google Scholar]
  15. Imhoff J. F. 1983; Rhodopseudomonas marina sp. nov., a new marine phototrophic purple bacterium. Syst. Appl. Microbiol. 4:512–521
    [Google Scholar]
  16. Imhoff J. F., Trüper H. G., Pfennig N. 1984; Rearrangements of the species and genera of the phototrophic “purple nonsulfur bacteria.” Int. J. Syst. Bacteriol. 34:340–343
    [Google Scholar]
  17. Janssen P. H., Harfoot C. G. 1991; Rhodopseudomonas rosea sp. nov., a new purple nonsulfur bacterium. Int. J. Syst. Bacteriol. 41:26–30
    [Google Scholar]
  18. Kawasaki H., Hoshino Y., Hirata A., Yamasato K. 1993; Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. Arch. Microbiol. 160:358–362
    [Google Scholar]
  19. Keppen O.L, Gorlenko V. M. 1975; A new species of purple budding bacteria containing bacteriochlorophyll b. Mikrobiologiya 44:258–264 (In Russian.)
    [Google Scholar]
  20. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111–120
    [Google Scholar]
  21. Klemme J. H., Chyla I., Preuss M. 1980; Dissimilatory nitrate reduction by strains of the facultative phototrophic bacterium Rhodopseudomonas palustris. FEMS Microbiol. Lett. 9:137–140
    [Google Scholar]
  22. Kompantseva E. I. 1989; A new species of the budding purple bacterium Rhodopseudomonas julia sp. nov. Mikrobiogiya 58:319–325 (In Russian.)
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  24. Orso S., Gouy M., Navarro E., Normand P. 1994; Molecular phylogenetic analysis of Nitrobacter spp. Int. J. Syst. Bacteriol. 44:83–86
    [Google Scholar]
  25. Pellelin N. B., Gest H. 1983; Diagnostic features of the photosynthetic bacterium Rhodopseudomonas sphaeroides. Curr. Microbiol. 9:339–344
    [Google Scholar]
  26. Pfennig N. 1969; Rhodopseudomonas acidophila, sp. n., a new species of budding purple nonsulfur bacteria. J. Bacteriol. 99:597–602
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  28. Satoh T., Hoshino Y., Kitamura H. 1976; Rhodopseudomonas sphaeroides forma sp. denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides. Arch. Microbiol. 108:265–269
    [Google Scholar]
  29. Seewaldt E., Schleifer K. H., Bock E., Stackebrandt E. 1982; The close phylogenetic relationship of Nitrobacter and Rhodopseudomonas palustris. Arch. Microbiol. 131:287–290
    [Google Scholar]
  30. Shen J., Hirayama O. 1991; Hydrogen photoproduction and denitrification by photosynthetic bacteria isolated from lake Nakaumi and its vicinity. J. Ferment. Bioeng. 72:338–342
    [Google Scholar]
  31. Siefert E., Irgens R. L., Pfennig N. 1978; Phototrophic purple and green bacteria in a sewage treatment plant. Appl. Environ. Microbiol. 35:38–44
    [Google Scholar]
  32. Trüper H. G., Imhoff J. F. 1989; Genus Rhodopseudomonas Kluyver and van Niel in Czurda and Maresch 1937, 119AL. 1672–1677 In Staley J. T., Bryant M. P., Pfennig N., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 3 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  33. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
  34. Woese C. R., Stackebrandt E., Weisburg W. G., Paster B. J., Madigan M. T., Fowler V. J., Hahn C. M., Blanz P., Gupta R., Nealson K. H., Fox G. E. 1984; The phylogeny of purple bacteria: the alpha subdivision. Syst. Appl. Microbiol. 5:315–326
    [Google Scholar]
  35. Wong F. Y. K., Stackebrandt E., Ladha J. K., Fleischman D. E., Date R. A., Fuerst J. A. 1994; Phylogenetic analysis of Bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from Aeschynomene species grown in separated geographical regions. Appl. Environ. Microbiol. 60:940–946
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-4-665
Loading
/content/journal/ijsem/10.1099/00207713-44-4-665
Loading

Data & Media loading...

Most cited Most Cited RSS feed