1887

Abstract

Abstract

Ninety genotypically characterized strains, including members of all 14 currently established genospecies, were studied by performing gas-liquid chromatographic analysis of their cellular fatty acid methyl esters (FAMEs). A total of 44 fatty acids and two alcohols were found in members of the genus All 90 strains contained 12:0, 13:0 iso, 14:0, 15:0 iso 30H, 16:0, 16:1 ω7, 17:0 iso, iso 17:1 ω9, summed feature 3 (16:1 iso I and/or 14:0 30H), and summed feature 7 (18:1 ω7, 18:1 ω, and/or 18:1 ω12), whereas all but one strain (99%) also contained 15:0 iso. Although the FAME profiles were very similar, minor quantitative variations could be used to differentiate phenospecies and/or hybridization groups. A cluster analysis of the mean data revealed five FAME clusters, which were compared with phenotypic and genotypic groups identified in the genus Hybridization groups that constituted the complex, the complex, and the complex were basically grouped into distinct FAME clusters. The taxonomic positions of hybridization groups 7 and 11 in these clusters, however, remained unclear. All of our results were highly reproducible. A new database of FAME fingerprints was generated, and this database can be used for rapid identification of unknown aeromonads. Using a large set of well-characterized aeromonads, we demonstrated for the first time that gas-liquid chromatographic FAME analysis can be used to differentiate the majority of the phenospecies and/or hybridization groups in the genus .

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1994-10-01
2022-08-08
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