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Methanolobus bombayensis B-1T (= OCM 438T) (T = type strain) was isolated from Arabian Sea sediments obtained near Bombay, India. This strain grew on methylamines, methanol, and dimethyl sulfide, but it did not catabolize H2-CO2, acetate, or formate. The cells were nonmotile, irregular coccoids (diameter, 1.0 to 1.5 µm) and occurred singly. Electron micrographs revealed that a cell membrane and a protein cell wall were present. The cells grew fastest at mesophilic temperatures, at a neutral pH, and at salinity levels near the salinity level of the ocean, and they required about 30 mM divalent cations (Mg2+ and Ca2+). The cells grew in mineral medium, but growth was greatly stimulated by yeast extract and peptones. The guanine-plus-cytosine content of the DNA was 39.2 ± 0.1 mol%. A comparison of 16S rRNA sequences showed that strain B-1T was phylogenetically related to Methanolobus vulcani, but the sequences of these organisms differed by 2%.
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