Identification of the Species Group with RI Fragments Containing rRNA Sequences and Description of sp. nov. Free

Abstract

Abstract

Strains of a new species, , were isolated from food and a variety of mammals. This species was recognized on the basis of the results of an analysis of genomic RI fragments containing portions of the rRNA operons. The patterns of hybridized fragments obtained from strains belonging to the new taxon were sorted into a distinguishable cluster and were distinct from the and patterns. The results of DNA-DNA hybridization reactions demonstrated that strains in this cluster were more closely related to and than to other species and yet were significantly different. While these strains had some of the phenotypic characteristics of the species group, the newly recognized taxon could be distinguished by its very small colonies on P agar, absence of alkaline phosphatase activity, and lack of acid production from -arabinose, maltose, -acetylglucosamine, -mannose, and raffinose. The type strain of the new species is strain DD 756 (= ATCC 51145).

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1994-01-01
2024-03-28
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References

  1. Albersheim J. W., Nevins D. J., English P. D., Karr A. 1967; A method for the analysis of sugars in plant cell wall polysaccharide by gas liquid chromatography. Carbohydr. Res. 5:340–345
    [Google Scholar]
  2. Ames B. N. 1966; Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 8:115–118
    [Google Scholar]
  3. Anderson A. J., Green R. S., Archibald A. R. 1977; Specific determination of ribitol teichoic acid in whole bacteria and isolated walls of Bacillus subtilis W23. Carbohydr. Res. 57:C7–C10
    [Google Scholar]
  4. Bergmeyer H. U. 1974 Methoden der enzymatischen Analyse Verlag Chemie; Weinheim, Germany:
    [Google Scholar]
  5. Brenner D. J., Fanning G. R., Rake A., Johnson K. E. 1969; A batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem. 28:447–459
    [Google Scholar]
  6. Brosius J., Ullrich A., Raker M. A., Gray A., Dull T. J., Gutell R. R., Noller H. F. 1981; Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA Operon of E. coli. Plasmid 6:112–118
    [Google Scholar]
  7. Chesneau O., Morvan A., Grimont F., Labischinski H., El Solh N. 1993; Staphylococcus pasteuri sp. nov., isolated from human, animal, and food specimens. Int. J. Syst. Bacteriol. 43:237–244
    [Google Scholar]
  8. De Buyser M.-L., Morvan A., Aubert S., Dilasser F., El Solh N. 1992; Evaluation of ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus. J. Gen. Microbiol. 138:889–899
    [Google Scholar]
  9. De Buyser M.-L., Morvan A., Grimont F., El Solh N. 1989; Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns. J. Gen. Microbiol. 135:989–999
    [Google Scholar]
  10. Faller A. H., Schleifer K. H. 1981; Modified oxidase and benzidine tests for the separation of staphylococci from micrococci. J. Clin. Microbiol. 13:1031–1035
    [Google Scholar]
  11. Glauner B., Holtje J. V., Schwarz U. 1988; The composition of the murein of Escherichia coli. J. Biol. Chem. 263:10088–10095
    [Google Scholar]
  12. Grimont F., Grimont P. A. D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann. Inst. Pasteur/Microbiol. (Paris) 137B:165–175
    [Google Scholar]
  13. Hébert G. A., Crowder C. G., Hancock G. A., Jarvis W. R., Thornsberry C. 1988; Characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family Micrococcaceae. J. Clin. Microbiol. 26:1939–1949
    [Google Scholar]
  14. Hubner R. December 1989 U.S. patent 4,885,697
    [Google Scholar]
  15. Ishikawa E., Imagawa M., Hashida S., Yoshitake S., Hmaguchi Y., Ueno T. 1983; Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining. J. Immunoassay 4:209–327
    [Google Scholar]
  16. Ishikawa E., Yamada Y., Yoshitake S., Kawaguchi H. 1981; A more stable maleimide, N-(4-carboxycyclohexylmethyl) maleimide for enzyme labeling. 90–105 In Ishikawa E., Kawai T., Miyai K. (ed.) Enzyme immunoassay Igaku-Shoin; Tokyo:
    [Google Scholar]
  17. Kaya S., Araki Y., Ito E. 1985; Characterization of a novel linkage unit between ribitol teichoic acid and peptidoglycan in Listeria monocytogenes cell walls. Eur. J. Biochem. 146:517–522
    [Google Scholar]
  18. Kloos W. E., Lambe D. W. Jr. 1991; Staphylococcus. 222–237 In Balows A., Hausier W. J. Jr., Herrmann K. L., Isenberg H. D., Shadomy H. J. (ed.) Manual of clinical microbiology, 5th ed.. American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  19. Kloos W. E., Schleifer K. H. 1975; Simplified scheme for routine identification of human Staphylococcus species. J. Clin. Microbiol. 1:82–88
    [Google Scholar]
  20. Kloos W. E., Schleifer K. H. 1986; Genus IV. Staphylococcus. 1013–1035 In Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 2 Williams and Wilkins; Baltimore:
    [Google Scholar]
  21. Kloos W. E., Schleifer K. H., Smith R. F. 1976; Characterization of Staphylococcus sciuri sp. nov. and its subspecies. Int. J. Syst. Bacteriol. 26:22–37
    [Google Scholar]
  22. Kloos W. E., Tornabene T. G., Schleifer K. H. 1974; Isolation and characterization of micrococci from human skin, including two new species, Micrococcus lylae and Micrococcus kristinae. Int. J. Syst. Bacteriol. 24:79–101
    [Google Scholar]
  23. Kloos W. E., Wolfshohl J. F. 1979; Evidence for deoxyribo-nucleotide sequence divergence between staphylococci living on human and other primate skin. Curr. Microbiol. 3:167–172
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 4:109–118
    [Google Scholar]
  25. Moeller V. 1955; Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol. Microbiol. Scand. 36:158–172
    [Google Scholar]
  26. Razin S., Gross M., Wormser M., Pollack Y., Glasser G. 1984; Detection of mycoplasmas infecting cell cultures by DNA hybridization. In Vitro (Rockville) 20:404–408
    [Google Scholar]
  27. Schleifer K. H. 1986; Taxonomy of coagulase-negative staphylococci. 11–26 In Mardh P. A., Schleifer K. H. (ed.) Coagulase-negative staphylococci Almqvist and Wiksell International; Stockholm:
    [Google Scholar]
  28. Schleifer K. H., Kandier O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  29. Thomson-Carter F. M., Carter P. E., Pennington T. H. 1989; Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns. J. Gen. Microbiol. 135:2093–2097
    [Google Scholar]
  30. Verdlov E. D., Monastyrakya G. S., Guskova L. I., Levitan T. L., Sheichenko V. I., Budowsky E. I. 1974; Modification of cytidine residues with a bisulfite–O-methylhydroxylamine mixture. Biochim. Biophys. Acta 340:153–165
    [Google Scholar]
  31. Webster J. A. April 1983 European Patent Office Application 82305061.2
    [Google Scholar]
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