1887

Abstract

Abstract

Cellular fatty acid profiles were determined for species in 33 genera of anaerobic gram-negative bacilli and were confirmed to be a useful taxonomic tool. Most of the genera could be differentiated by visual inspection of their profiles. The three genus pairs that were most difficult to distinguish visually ( and and and and ) and the species of these genera were differentiated by the MIDI (Microbial ID, Inc.) identification system. Similarities in cellular fatty acid profiles may be correlated with similarities in other phenotypic characteristics, but more often there is no other obvious phenotypic relationship. Although medium components may not change the constituents detected or the ratios among the constituents detected for some species, identical medium changes may result in vast differences in the profiles obtained with other species. Thus, if a worker wishes to compare profiles of various taxa, it is essential that the same cultural and analytical conditions be used.

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1994-04-01
2023-02-01
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References

  1. Adriaans B., Shah H. 1988; Fusobacterium ulcerans sp. nov. from tropical ulcers. Int. J. Syst. Bacteriol. 38 447 448
    [Google Scholar]
  2. Baron E. J., Summanen P., Downes J., Roberts M. C., Wexler H., Finegold S. M. 1989; Bilophila wadsworthia, gen. nov. and sp. nov., a unique gram-negative anaerobic rod recovered from appendicitis specimens and human faeces. J. Gen. Microbiol. 135 3405 3411
    [Google Scholar]
  3. Benjamin J., Leaper S., Owens R. J., Skirrow M. B. 1983; Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. Curr. Microbiol. 8 231 238
    [Google Scholar]
  4. Brondz I., Olsen I. 1991; Multivariate analyses of cellular fatty acids in Bacteroides, Prevotella, Porphyromonas, Wolinella, and Campylobacter spp. J. Clin. Microbiol. 29 183 189
    [Google Scholar]
  5. Bryant M. P. 1984; Butyrivibrio Bryant and Small 1956, 18, emend. Moore, Johnson and Holdeman 1976, 241AL . 641 643 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  6. Bryant M. P. 1984; Succinimonas Bryant, Small, Bouma and Chu 1958, 21AL,. 643 644 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  7. Bryant M. P. 1984; Succinivibrio Bryant and Small 1956, 22AL,. 644 645 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  8. Bryant M. P. 1984; Anaerobiospirillum Davis, Cleven, Brown and Balish 1976,503AL,. 645 646 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  9. Bryant M. P. 1984; Selenomonas Von Prowazek 1913, 36AL,. 650 653 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  10. Calhoon D. A., Mayberry W. R., Slots J. 1983; Cellular fatty acid and soluble protein profiles of oral fusobacteria. J. Dent. Res. 62 1181 1185
    [Google Scholar]
  11. Cato E. P., Moore L. V. H., Moore W. E. C. 1985; Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus. Int. J. Syst. Bacteriol. 35 475 477
    [Google Scholar]
  12. Collins M. D., Shah H. N. 1986; Reclassification of Bacteroides praeacutus Tissier (Holdeman and Moore) in a new genus, Tissierella, as Tissierella praeacuta comb. nov. Int. J. Syst. Bacteriol. 36 461 163
    [Google Scholar]
  13. Collins M. D., Shah H. N., Mitsuoka T. 1985; Reclassification of Bacteroides microfusus (Kaneuchi and Mitsuoka) in a new genus, Rikenella, as Rikenella microfusus comb. nov. Syst. Appl. Microbiol. 6 79 81
    [Google Scholar]
  14. Dewhirst F. E., Paster B. J., La Fontaine S., Rood J. I. 1990; Transfer of Kingella indologenes (Snell and Lapage 1976) to the genus Suttonella gen. nov. as Suttonella indologenes comb, nov.; transfer of Bacteroides nodosus (Beveridge 1941) to the genus Dichelobacter gen. nov. as Dichelobacter nodosus comb, nov.; and assignment of the genera Cardiobacterium, Dichelobacter, and Suttonella to Cardiobacteriaceae fam. nov. in the gamma division of Proteobacteria on the basis of 16S rRNA sequence comparisons. Int. J. Syst. Bacteriol. 40 426 433
    [Google Scholar]
  15. Gebhart C. J., Edmonds P., Ward G. E., Kirtz H. J., Brenner D. J. 1985; “Campylobacter hyointestinalis” sp. nov.: a new species of Campylobacter found in the intestines of pigs and other animals. J. Clin. Microbiol. 21 715 720
    [Google Scholar]
  16. Hofstad T. 1984; Leptotrichia Trevisan 1879,138AL,. 637 641 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  17. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe laboratory manual , 4th ed.. VPI Anaerobe Laboratory; Blacksburg, Va:
    [Google Scholar]
  18. Holdeman L. V., Johnson J. L. 1977; Bacteroides disiens sp. nov. and Bacteroides bivius sp. nov. from human clinical infections. Int. J. Syst. Bacteriol. 27 337 345
    [Google Scholar]
  19. Holdeman L. V., Johnson J. L. 1982; Description of Bacteroides loescheii sp. nov. and emendation of the descriptions of Bacteroides melaninogenicus (Oliver and Wherry) Roy and Kelly 1939 and Bacteroides denticola Shah and Collins 1981. Int. J. Syst. Bacteriol. 32 399 409
    [Google Scholar]
  20. Holdeman L. V., Kelly R. W., Moore W. E. C. 1984; Genus 1. Bacteroides Castellani and Chalmers 1919, 959AL,. 604 631 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Holdeman L. V., Moore W. E. C., Churn P. C., Johnson J. L. 1982; Bacteroides oris and Bacteroides buccae, new species from human periodontitis and other human infections. Int. J. Syst. Bacteriol. 32 125 131
    [Google Scholar]
  22. Holt S. C., Kinder S. A. 1989; Genus II. Capnocytophaga Leadbetter, Holt and Socransky 1982,266VP,. 2025 2058 Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Bergey’s manual of systematic bacteriology 3 The Williams and Wilkins Co.; Baltimore:
    [Google Scholar]
  23. Ifkovits R. W., Ragheb H. S. 1968; Cellular fatty acid composition and identification of rumen bacteria. Appl. Microbiol. 16 1406 1413
    [Google Scholar]
  24. Jackson F. L., Goodman Y. 1984; Eikenella Jackson and Goodman 1972, 74AL,. 591 597 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  25. Johnson J. L. 1978; Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species. Int. J. Syst. Bacteriol. 28 245 256
    [Google Scholar]
  26. Johnson J. L. Personal communication
    [Google Scholar]
  27. Johnson J. L., Harich B. 1986; Ribosomal ribonucleic acid homology among species of the genus Bacteroides. Int. J. Syst. Bacteriol. 36 71 79
    [Google Scholar]
  28. Johnson J. L., Holdeman L. V. 1983; Bacteroides intermedius comb. nov. and descriptions of Bacteroides corporis sp. nov. and Bacteroides levii sp. nov. Int. J. Syst. Bacteriol. 33 15 25
    [Google Scholar]
  29. Johnson J. L., Holdeman L. V., Moore W. E. C. 1985; Replacement of the type strain of Selenomonas sputigena under Rule 18g. Int. J. Syst. Bacteriol. 35 371 374
    [Google Scholar]
  30. Johnson J. L., Moore W. E. C., Moore L. V. H. 1986; Bacteroides caccae sp. nov., Bacteroides merdae sp. nov., and Bacteroides stercoris sp. nov. isolated from human feces. Int. J. Syst. Bacteriol. 36 499 501
    [Google Scholar]
  31. Lai C.-H., Males M., Dougherty P. A., Berthold P., Listgarten M. A. 1983; Centipeda periodontii gen. nov., sp. nov. from human periodontal lesions. Int. J. Syst. Bacteriol. 33 628 635
    [Google Scholar]
  32. Lambe D. W. Jr., Ferguson K. P., Mayberry W. R. 1982; Characterization of Bacteroides gingivalis by direct fluorescent antibody staining and cellular fatty acid profiles. Can. J. Microbiol. 28 367 374
    [Google Scholar]
  33. Lee S. Y. 1984; Pectinatus Lee, Mabee and Jangaard 1978, 582AL,. 655 658 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  34. Love D. N., Bailey G. D., Collings S., Briscoe D. A. 1992; Description of Porphyromonas circumdentaria sp. nov. and reassignment of Bacteroides salivosus (Love, Johnson, Jones, and Calverley 1987) as Porphyromonas (Shah and Collins 1988) salivosa comb. nov. Int. J. Syst. Bacteriol. 42 434 438
    [Google Scholar]
  35. Mayberry W. R., Lambe D. W. Jr., Ferguson K. P. 1982; Identification of Bacteroides species by cellular fatty acid profiles. Int. J. Syst. Bacteriol. 32 21 27
    [Google Scholar]
  36. Miyagawa E., Azuma R., Suto T. 1979; Cellular fatty acid composition in gram-negative obligately anaerobic rods. J. Gen. Microbiol. 25 41 51
    [Google Scholar]
  37. Montgomery L., Flesher B., Stahl D. 1988; Transfer of Bacteroides succinogenes (Hungate) to Fibrobacter gen. nov. as Fibrobacter succinogenes comb. nov. and description of Fibrobacter intestinalis sp. nov. Int. J. Syst. Bacteriol. 38 430 435
    [Google Scholar]
  38. Moore L. V. H., Johnson J. L., Moore W. E. C. 1987; Selenomonas noxia sp. nov., Selenomonas flueggei sp. nov., Selenomonas infelix sp. nov., Selenomonas dianae sp. nov., and Selenomonas artemidis sp. nov. from the human gingival crevice. Int. J. Syst. Bacteriol. 37 271 280
    [Google Scholar]
  39. Moore L. V. H., Moore W. E. C. 1993 Anaerobe laboratory manual update, 1993 VPI Anaerobe Laboratory; Blacksburg, Va:
    [Google Scholar]
  40. Moore L. V. H., Moore W. E. C. 1994; Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallelia seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb, nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. Int. J. Syst. Bacteriol. 44 187 192
    [Google Scholar]
  41. Moore W. E. C., Hash D. E., Holdeman L. V., Cato E. P. 1980; Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl. Environ. Microbiol. 39 900 907
    [Google Scholar]
  42. Moore W. E. C., Holdeman L. V. 1984; Genus Desulfomonas Moore, Johnson and Holdeman 1976, 238AL,. 672 673 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  43. Moore W. E. C., Holdeman L. V., Kelley R. W. 1984; Genus II. Fusobacterium Knorr 1922, 4AL,. 631 637 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  44. Prins R. A. 1984; Anaerovibrio Hungate 1966, 80AL,. 653 655 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  45. Sandstedt K., Ursing J. 1991; Description of Campylobacter upsaliensis sp. nov. previously known as the CNW group. Syst. Appl. Microbiol. 14 39 45
    [Google Scholar]
  46. Shah H. N., Collins M. D. 1980; Fatty acid and isoprenoid quinone composition in the classification of Bacteroides melaninogenicus and related taxa. J. Appl. Bacteriol. 48 75 87
    [Google Scholar]
  47. Shah H. N., Collins M. D. 1982; Reclassification of Bacteroides hypermegas (Harrison and Hansen) in a new genus, Megamonas, as Megamonas hypermegas comb. nov. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 3 394 389
    [Google Scholar]
  48. Shah H. N., Collins M. D. 1982; Reclassification of Bacteroides multiacidus (Mitsuokella, Terada, Watanabe, and Uchida) in a new genus Mitsuokella, as Mitsuokella multiacidus comb. nov. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe C 3 491 494
    [Google Scholar]
  49. Shah H. N., Collins M. D. 1986; Reclassification of Bacteroides furcosus Veillon and Zuber (Hauduroy, Ehringer, Urbain, Guillot and Magrou) in a new genus Anaerorhabdus, as Anaerorhabdus furcosus comb. nov. Syst. Appl. Microbiol. 8 86 88
    [Google Scholar]
  50. Shah H. N., Collins M. D. 1988; Proposal for reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a new genus, Porphyromonas. Int. J. Syst. Bacteriol. 38 128 131
    [Google Scholar]
  51. Shah H. N., Collins M. D. 1989; Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int. J. Syst. Bacteriol. 39 85 87
    [Google Scholar]
  52. Shah H. N., Collins M. D. 1990; Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int. J. Syst. Bacteriol. 40 205 208
    [Google Scholar]
  53. Shah H. N., Collins M. D., Watanabe J., Mitsuoka T. 1985; Bacteroides oulorum sp. nov., a nonpigmented saccharolytic species from the oral cavity. Int. J. Syst. Bacteriol. 35 193 197
    [Google Scholar]
  54. Shah H. N., Gharbia S. E. 1992; Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov. Int. J. Syst. Bacteriol. 42 542 546
    [Google Scholar]
  55. Slots J., Potts T. V., Mashimo P. A. 1983; Fusobacterium periodonticum, a new species from the human oral cavity. J. Dent. Res. 62 960 963
    [Google Scholar]
  56. Smibert R. M. 1984; Treponema Schaudinn 1905, 1728AL,. 49 57 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  57. Smibert R. M. 1984; Campylobacter Sebald and Véron 1963, 907AL,. 111 118 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  58. Stackebrandt E., Hippe H. 1986; Transfer of Bacteroides amylophilus to a new genus Ruminobacter gen. nov., nom. rev. as Ruminobacter amylophilus comb. nov. Syst. Appl. Microbiol. 8 204 207
    [Google Scholar]
  59. Stanton T. B. 1992; Proposal to change the genus designation of Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. Int. J. Syst. Bacteriol. 42 189 190
    [Google Scholar]
  60. Tanner A. C. R., Listgarten M. A., Eberson J. L. 1984; Wolinella curva sp. nov.: “Vibrio succinogenes” of human origin. Int. J. Syst. Bacteriol. 34 275 282
    [Google Scholar]
  61. Tanner A. C. R., Socransky S. S. 1984; Genus VIII. Wolinella Tanner, Badger, Listgarten, Visconti and Socransky 1981, 439VP,. 646 651 Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  62. Vainshtein M., Hippe H., Kroppenstedt R. M. 1992; Cellular fatty acid composition of Desulfovibrio species and its use in classification of sulfate-reducing bacteria. Syst. Appl. Microbiol. 15 554 566
    [Google Scholar]
  63. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Archobacter gen. nov. Int. J. Syst. Bacteriol. 41 88 103
    [Google Scholar]
  64. Watanabe J., Benno Y., Mitsuoka T. 1983; Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int. J. Syst. Bacteriol. 33 57 64
    [Google Scholar]
  65. Wu C. C., Johnson J. L., Moore W. E. C., Moore L. V. H. 1992; Emended descriptions of Prevotella denticola, Prevotella loescheii, Prevotella veroralis, and Prevotella melaninogenica. Int. J. Syst. Bacteriol. 42 536 541
    [Google Scholar]
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