1887

Abstract

Abstract

A comparative analysis of seven new 16S rRNA gene sequences of pathogenic species with previously published vibrio sequences confirmed that represents a group that is not closely related to the core organisms of the genus In addition, we found that , () and branch off separately from the core group. A comparison of the 16S rRNA gene sequences of strains belonging to biotypes 1 and 2 revealed that the sequences of all but four biotype 1 strains were identical to each other but slightly different (17 bases) from the sequences of the rest of the strains investigated. In addition, the sequences of variable regions of the 23S rRNA genes of and C7184 and TW1 were determined, aligned, and compared with all available bacterial 23S rRNA sequences in order to search for specific target sites. As a result, four oligonucleotide probes specific for were synthesized, and the specificities of these probes were evaluated by dot blot hybridization to membrane-bound RNAs from 21 strains, 13 strains belonging to other species, 61 strains belonging to species that are members of the alpha, beta, and gamma subclasses of the and 3 eucaryotic microorganisms. Two probes hybridized with all of the strains tested, and the other two probes distinguished biotype 1 strains from all other organisms. In situ identification of by using tetramethylrhodamine- or fluorescein-labelled oligonucleotides is now possible.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-2-330
1994-04-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/44/2/ijs-44-2-330.html?itemId=/content/journal/ijsem/10.1099/00207713-44-2-330&mimeType=html&fmt=ahah

References

  1. Amann R. I., Binder B. J., Olson R. J., Chrisholm S. W., Devereux R., Stahl A. D. 1990; Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56 1919 1925
    [Google Scholar]
  2. Amann R. I., Krumholz L., Stahl D. A. 1990; Fluorescent oligonucleotide probing of whole cells for determinative phylogenetic and environmental studies in microbiology. J. Bacteriol. 172 762 770
    [Google Scholar]
  3. Amaro C., Biosca E. G., Fouz B., Garay E. 1992; Electrophoretic analysis of heterogeneous lipopolysaccharides from various strains of Vibrio vulnificus biotypes 1 and 2 by silver staining and immunoblotting. Curr. Microbiol. 25 99 104
    [Google Scholar]
  4. Baumann L., Bang S. S., Baumann P. 1980; Study of relationship among species of Vibrio, Photobacterium, and terrestrial enterobacteria by an immunological comparison of glutamine synthetase and superoxide dismutase. Curr. Microbiol. 4 133 138
    [Google Scholar]
  5. Baumann P., Baumann L., Reichelt J. L. 1973; Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica. J. Bacteriol. 113 1144 1155
    [Google Scholar]
  6. Betzl D., Ludwig W., Schleifer K. H. 1990; Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 56 2927 2929
    [Google Scholar]
  7. Biosca E. G., Amaro C., Esteve C., Alcaide E., Garay E. 1991; First record of Vibrio vulnificus biotype 2 from diseased European eel, Anguilla anguilla L. J. Fish Dis. 14 103 109
    [Google Scholar]
  8. Brauns L. A., Hudson M. C., Oliver J. D. 1991; Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells. Appl. Environ. Microbiol. 57 2651 2655
    [Google Scholar]
  9. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148 107 127
    [Google Scholar]
  10. Coyne V. E., Pillidge C. J., Sledjeski D. D., Hori H., Ortiz-Conde B. A., Muir D. G., Weiner R. M., Colwell R. R. 1989; Reclassification of Alteromonas colwelliana to the genus Shewanella by DNA-DNA hybridization, serology and 5S ribosomal RNA sequence data. Syst. Appl. Microbiol. 12 275 279
    [Google Scholar]
  11. DeLong E. F., Wickham G. S., Pace N. R. 1989; Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 243 1360 1363
    [Google Scholar]
  12. Dorsch M., Lane D., Stackebrandt E. 1992; Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences. Int. J. Syst. Bacteriol. 42 58 63
    [Google Scholar]
  13. Farmer J. J. III 1980; Revival of the name Vibrio vulnificus. Int. J. Syst. Bacteriol. 30 656
    [Google Scholar]
  14. Felsenstein J. 1982; Numerical methods for inferring phylogenetic trees. Q. Rev. Biol. 57 379 404
    [Google Scholar]
  15. Hertel C., Ludwig W., Obst M., Vogel R. F., Hammes W. P., Schleifer K. H. 1991; 23S rRNA-targeted oligonucleotide probes for the rapid identification of meat lactobacilli. Syst. Appl. Microbiol. 14 173 177
    [Google Scholar]
  16. Hill W. E., Keasler S. P., Trucksess M. W., Feng P., Kaysner C. A., Lampei K. A. 1991; Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters. Appl. Environ. Microbiol. 57 707 711
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules,. 21 132 Munro H. N. Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  18. Kita-Tsukamoto K., Oyaizu H., Nanba K., Simidu U. 1993; Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int. J. Syst. Bacteriol. 43 8 19
    [Google Scholar]
  19. Ludwig W., Kirchhof G., Klugbauer N., Weizenegger M., Betzl D., Ehrmann M., Hertel C., Jilig S., Tatzel R., Zitzeisberger H., Liebl S., Hochberger M., Lane D., Wallnöfer P. R., Schleifer K. H. 1993; Complete 23S ribosomal RNA sequences of gram-positive bacteria with a low DNA G+C content. Syst. Appl. Microbiol. 15 487 501
    [Google Scholar]
  20. MacDonell M. T., Colwell R. R. 1985; Phylogeny of Vibrionaceae and recommendation for two new genera, Listonella and Shewanella. Syst. Appl. Microbiol. 6 171 182
    [Google Scholar]
  21. Morris J. G. Jr., Wright A. C., Roberts D. R., Wood P. K., Simpson L. M., Oliver J. D. 1987; Identification of environmental Vibrio vulnificus isolates with a DNA probe for the cytotoxinhemolysin gene. Appl. Environ. Microbiol. 53 193 195
    [Google Scholar]
  22. Olsen G. J., Overbeek R., Larsen N., Marsh T. L., McCaughey M. J., Maciukenas M. A., Kuan W. M., Macke T. J., Xing Y., Woese C. R. 1992; The ribosomal database project. Nucleic Acids Res. 20 (Suppl.) 2199 2200
    [Google Scholar]
  23. O’Neil K. R., Jones S. H., Grimes D. J. 1992; Seasonal incidence of Vibrio vulnificus in the Great Bay Estuary of New Hampshire and Maine. Appl. Environ. Microbiol. 58 3257 3262
    [Google Scholar]
  24. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8 151 156
    [Google Scholar]
  25. Reichelt J. L., Baumann P., Baumann L. 1976; Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch. Microbiol. 110 101 120
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 406 425
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual , 2nd ed.. Cold Spring Harbor Laboratory, Cold Spring Harbor; N.Y.:
    [Google Scholar]
  28. Schleifer K. H., Ludwig W., Amann R. 1993; Nucleic acid probes,. 463 509 Goodfellow M., O’Donnell A. O. Handbook of new bacterial systematics Academic Press; London:
    [Google Scholar]
  29. Stelma G. N. Jr., Reyes A. L., Peeler J. T., Johnson C. H., Spaulding P. L. 1992; Virulence characteristics of clinical and environmental isolates of Vibrio vulnificus. Appl. Environ. Microbiol. 58 2776 2782
    [Google Scholar]
  30. Tison D. L., Nishibuchi M., Greenwood J. D., Seidler R. J. 1982; Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl. Environ. Microbiol. 44 640 646
    [Google Scholar]
  31. Tison D. L., Seidler R. J. 1981; Genetic relatedness of clinical and environmental isolates of lactose-positive Vibrio vulnificus. Curr. Microbiol. 6 181 184
    [Google Scholar]
  32. Westerdahl A., Amann R. I. Unpublished data
/content/journal/ijsem/10.1099/00207713-44-2-330
Loading
/content/journal/ijsem/10.1099/00207713-44-2-330
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error