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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 44, Issue 2

Sequence Determination of rRNA Genes of Pathogenic Species and Whole-Cell Identification of with rRNA-Targeted Oligonucleotide Probes Free

Abstract

Abstract

A comparative analysis of seven new 16S rRNA gene sequences of pathogenic species with previously published vibrio sequences confirmed that represents a group that is not closely related to the core organisms of the genus In addition, we found that , () and branch off separately from the core group. A comparison of the 16S rRNA gene sequences of strains belonging to biotypes 1 and 2 revealed that the sequences of all but four biotype 1 strains were identical to each other but slightly different (17 bases) from the sequences of the rest of the strains investigated. In addition, the sequences of variable regions of the 23S rRNA genes of and C7184 and TW1 were determined, aligned, and compared with all available bacterial 23S rRNA sequences in order to search for specific target sites. As a result, four oligonucleotide probes specific for were synthesized, and the specificities of these probes were evaluated by dot blot hybridization to membrane-bound RNAs from 21 strains, 13 strains belonging to other species, 61 strains belonging to species that are members of the alpha, beta, and gamma subclasses of the and 3 eucaryotic microorganisms. Two probes hybridized with all of the strains tested, and the other two probes distinguished biotype 1 strains from all other organisms. In situ identification of by using tetramethylrhodamine- or fluorescein-labelled oligonucleotides is now possible.

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Copyright 1994, International Union of Microbiological Societies
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1994-04-01
2024-04-20
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Sequence Determination of rRNA Genes of Pathogenic Vibrio Species and Whole-Cell Identification of Vibrio vulnificus with rRNA-Targeted Oligonucleotide Probes
Int J Syst Evol Microbiol 44, 330 (1994); https://doi.org/10.1099/00207713-44-2-330
/content/journal/ijsem/10.1099/00207713-44-2-330
/content/journal/ijsem/10.1099/00207713-44-2-330
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