1887

Abstract

Variation among isolates of was examined by using restriction fragmentation patterns and the PCR performed with arbitrary and sequence-specific oligonucleotide primers. The patterns were compared with the patterns generated from strains of closely related species belonging to the “ group” of bacteria, including , and . All profiles were identical for each of 18 restriction enzymes, each of 10 arbitrary PCR primers, and a repetitive extragenic palindrome-specific PCR primer. The PCR profiles generated with a coliphage M13-based primer exhibited slight pattern variation in a 400- to 500-bp band region. The profiles were unique compared with the profiles of the other species examined. In these other species, strain-to-strain variations were observed. Our results showed that isolates of are almost completely homogeneous, indicating a clonal lineage, and are distinct from other members of the group and that , as a species in its own right, may have evolved only relatively recently.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-1-99
1994-01-01
2019-10-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-1-99
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error