A total of 14 field strains originally labeled , 4 field strains of , and 12 type strains, as well as four additional reference strains, were classified by performing a restriction endonuclease analysis of chromosomal DNAs digested with 718, I, and RI. The patterns were analyzed by using (i) Jaccard coefficients and the unweighted pair group algorithm with arithmetic averages and (ii) principal-component analysis (PCA) and soft independent modeling of class analogy (SIMCA). Grouping by using the Jaccard coefficients and the unweighted pair group algorithm with arithmetic averages resulted in six clusters, defined at a similarity level of 69%. Cluster 1 comprised 10 field strains of sp. strain DSM 20056, and DSM 20016 (T = type strain) and could be divided into four subclusters. Cluster 2 included two field strains. Cluster 3 included one field strain labeled and one field strain labeled . Cluster 4 could be divided into three subclusters and included two field strains labeled , one reference strain and the type strain of , and four additional type strains. Cluster 5 contained two strains. Cluster 6 included two type strains. The two numerical methods gave the same general results, but the PCA-SIMCA method resulted in a more complete view of the relationships. The SIMCA analysis grouped DSM 20016, strain DSM 20056, and, with one exception, all of the field strains as a “class.” DSM 20075 and subsp. DSM 20008 were the type strains most closely related to the SIMCA class. The strains isolated from rats could be separated from the strains isolated from humans and pigs by the PCA. DNA-DNA-hybridization showed that strains classified as on the basis of the results of the restriction enzyme analysis also exhibited >70% DNA-DNA-homology to the type strain of . The phenotype of (ability to ferment carbohydrates) coincided in most cases with the genotype; this seemed not to be the case for strains.


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