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DNA-DNA hybridization studies (with the S1 nuclease method [J. L. Johnson, Methods Microbiol. 18:33-74, 1985]) were performed on members of the genus Fusobacterium. Fusobacterium varium ATCC 8501Tshowed 88 and 79% DNA homology with F. pseudonecrophorum JCM 3722Tand JCM 3723, respectively, while F. pseudonecrophorum JCM 3722Tshowed 81 and 82% DNA homology with F. varium ATCC 8501Tand F. pseudonecrophorum JCM 3723, respectively. These genetic data and their similar phenotypic characteristics suggest that F. varium(Eggerth and Gagnon 1933) Moore and Holdeman 1969 and F. pseudonecrophorum(ex Prévot 1940) Shinjo et al. 1990 belong to a single species. We propose, therefore, that strains JCM 3722 and JCM 3723 of F. pseudonecrophorum be transferred to the species F. varium.
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