1887

Abstract

is a V factor-dependent species. A plasmid conferring V factor independence in and was transferred to plasmid-free Rd by DNA transformation. The growth characteristics of the transformants in a complex and a chemically defined medium were compared, and the ability of several exogenous pyridine nucleotides and precursors to support growth was examined. Although the transformants appeared to be V factor independent in a complex medium, in a chemically defined medium they exhibited both V factor-dependent and nicotinamide-dependent growth. Because of the inability of the plasmid-free Rd to utilize nicotinamide for growth, it was concluded that the genes conferring this function were plasmid linked. Our results indicate that the V factor requirement, as it is presently defined, is not suitable to serve as a definitive taxonomic criterion for species determination in the family

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-4-799
1993-10-01
2023-02-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/4/ijs-43-4-799.html?itemId=/content/journal/ijsem/10.1099/00207713-43-4-799&mimeType=html&fmt=ahah

References

  1. Alexander H. E., Leidy G. 1951; Determination of inherited traits of Haemophilus influenzae by deoxyribonucleic acid fractions isolated from type specific cells. J. Exp. Med. 93:345–349
    [Google Scholar]
  2. Berkman S. 1942; Accessory growth factor requirements of the members of the genus Pasteurella. J. Infect. Dis. 71:201–211
    [Google Scholar]
  3. Cynamon M. H., Sorg T. B., Patapow A. 1988; Utilization and metabolism of NAD by Haemophilus parainfluenzae. J. Gen. Microbiol. 134:2789–2799
    [Google Scholar]
  4. Foster J. W., Moat A. G. 1980; Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems. Microbiol. Rev. 44:83–105
    [Google Scholar]
  5. Gromkova R., Dangor Y., Miller S. D. 1989; V-factor (NAD)-independent Haemophilus parainfluenzae recovered from a human genital ulcer. Eur. J. Clin. Microbiol. Infect. Dis. 8:569 (Letter.)
    [Google Scholar]
  6. Gromkova R., Koornhof H. J. 1990; Naturally occurring NAD-independent Haemophilus parainfluenzae. J. Gen. Microbiol. 136:1031–1035
    [Google Scholar]
  7. Gromkova R. C., Rowji P. B., Koornhof H. J. 1989; Induction of competence in nonencapsulated and encapsulated strains of Haemophilus influenzae. Curr. Microbiol. 19:241–245
    [Google Scholar]
  8. Herriott R. M., Meyer E. Y., Vogt M., Modan M. 1970; Defined medium for growth of Haemophilus influenzae. J. Bacteriol. 101:513–516
    [Google Scholar]
  9. Kahn D. W., Anderson B. M. 1986; Characterization of Haemophilus influenzae nucleotide pyrophosphatase. An enzyme of critical importance for growth of the organism. J. Biol. Chem. 261:6016–6025
    [Google Scholar]
  10. Kasarov L. B., Moat A. G. 1973; Biosynthesis of NAD in Haemophilus haemoglobinophilus. Biochim. Biophys. Acta 320:372–378
    [Google Scholar]
  11. Kilian M. 1976; A taxonomic study of the genus Haemophilus with the proposal of a new species. J. Gen. Microbiol. 93:9–62
    [Google Scholar]
  12. Koser S. A., Berkman S., Dorfman A. 1941; Comparative activity of nicotinic acid and nicotinamide as growth factors for microorganisms. Proc. Soc. Exp. Biol. Med. 47:504–507
    [Google Scholar]
  13. Lwoff A., Lwoff M. 1937; Studies on codehydrogenases. I. Nature of growth factor “V.”. Proc. R. Soc. London B Biol. Sci. 122:352–359
    [Google Scholar]
  14. Niven D. F., Levesque M. 1988; V-factor-dependent growth of Actinobacillus pleuropneumoniae biotype 2 (Bert- schinger 2008/76). Int. J. Syst. Bacteriol. 38:319–320
    [Google Scholar]
  15. Niven D. F., O’Reilly T. 1990; Significance of V-factor dependency in the taxonomy of Haemophilus species and related organisms. Int. J. Syst. Bacteriol. 40:1–4
    [Google Scholar]
  16. Nsanze H., Plummer F. A., Maggwa A. B. N., Maitha G., Dylewski J., Piot P., Ronald A. R. 1984; Comparison of media for the primary isolation of Haemophilus ducreyi. Sex. Transm. Dis. 11:6–9
    [Google Scholar]
  17. Pohl S., Bertschinger H. U., Frederiksen W., Mannheim W. 1983; Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb, nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. Int. J. Syst. Bacteriol. 33:510–514
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory; Cold Spring Harbor, N.Y.:
    [Google Scholar]
  19. Wessman G. E. 1965; Cultivation of Pasteurella haemolytica in a casein hydrolysate medium. Appl. Microbiol. 13:426–431
    [Google Scholar]
  20. Wessman G. E. 1966; Cultivation of Pasteurella haemolytica in a chemically defined medium. Appl. Microbiol. 14:597–602
    [Google Scholar]
  21. Wessman G. E., Wessman G. 1970; Chemically defined media for Pasteurella multocida and Pasteurella ureae, and a comparison of their thiamine requirements with those of Pasteurella haemolytica. Can. J. Microbiol. 16:751–757
    [Google Scholar]
  22. Wessman G. E., Wessman G. 1972; Requirements for growth of Pasteurella ureae in a chemically defined medium. Can. J. Microbiol. 18:107–109
    [Google Scholar]
  23. Windsor H. M. Unpublished data
  24. Windsor H. M., Gromkova R. C., Koornhof H. J. 1991; Plasmid-mediated NAD independence in Haemophilus parain-fluenzae. J. Gen. Microbiol. 137:2415–2421
    [Google Scholar]
  25. Windsor H. M., Gromkova R. C., Koornhof H. J. 1993; Transformation of V-factor independence from Haemophilus ducreyi to Haemophilus influenzae and Haemophilus parainflu-enzae. Med. Microbiol. Lett. 2:159–167
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-4-799
Loading
/content/journal/ijsem/10.1099/00207713-43-4-799
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error