1887

Abstract

A mycoplasma designated strain 4229(T = type strain) was isolated in 1984 from the turbinate of a duck in France, and similar strains were isolated from geese in France and from a partridge in England. All of these strains were originally identified as by immunofluorescence and growth inhibition tests, but subsequent serological and molecular studies indicated only a partial relationship to this species and DNA-DNA hybridization studies revealed only approximately 40 to 46% genetic homology with PG31. In this study morphological, cultural, and physical investigations were carried out on strain 4229and partridge strain B2/85, after we first demonstrated the similarity between these organisms by performing a restriction enzyme analysis of their DNAs. Both strains had phenotypic properties very similar to properties, including the presence of an attachment organelle. As a result of these investigations, the organisms were assigned to the class , the order , and the genus They fermented glucose, reduced triphenyl tetrazolium chloride aerobically and anaerobically, and exhibited hemadsorption and hemagglutination, but other biochemical tests were negative. Apart from a serological cross-reaction with , these organisms exhibited no significant relationship with any previously described species as determined by growth inhibition or immunofluorescence tests or with a number of additional serovars and unclassified avian strains. This taxon therefore appears to be a new species, for which we propose the name The type strain is strain 4229 (= NCTC 11733 = ATCC 51306). The significance of the organism has not been fully investigated, but preliminary in vitro and in vivo studies have indicated that it may be pathogenic.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-4-721
1993-10-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/4/ijs-43-4-721.html?itemId=/content/journal/ijsem/10.1099/00207713-43-4-721&mimeType=html&fmt=ahah

References

  1. Aluotto B. B., Wittier R. G., Williams C. O., Faber J. E. 1970; Standardized bacteriologic techniques for the characterization of Mycoplasma species. Int. J. Syst. Bacteriol. 20:35–58
    [Google Scholar]
  2. Askaa G., Erno H. 1976; Elevation of Mycoplasma agalactiae subsp bovis to species rank: Mycoplasma bovis (Hale et al.) comb. nov. Int. J. Syst. Bacteriol. 26:322–325
    [Google Scholar]
  3. Bentina D., Dorrer D., Tadina T. 1987; Mycoplasma species isolated from six avian species. Avian Pathol. 16:653–664
    [Google Scholar]
  4. Bencina D., Tadina T., Dorrer D. 1988; Natural infection of ducks with Mycoplasma synoviae and Mycoplasma gallisepticum and Mycoplasma egg transmission. Avian Pathol. 17:441–449
    [Google Scholar]
  5. Bencina D., Tadina T., Dorrer D. 1988; Natural infection of geese with Mycoplasma gallisepticum and Mycoplasma synoviae and egg transmission of the mycoplasmas. Avian Pathol. 17:925–928
    [Google Scholar]
  6. Black F. T. 1973; Modification of the growth inhibition test and its application to human T-mycoplasmas. Appl. Microbiol. 25:528–533
    [Google Scholar]
  7. Bradbury J. M. 1977; Rapid biochemical tests for characterization of the Mycoplasmatales. J. Clin. Microbiol. 5:531–534
    [Google Scholar]
  8. Bradbuiy J. M. 1984; Avian mycoplasma infections: prototype of mixed infections with mycoplasmas, bacteria and viruses. Ann. Microbiol. (Inst. Pasteur) 135A:83–89
    [Google Scholar]
  9. Bradbury J. M. Unpublished data
  10. Bradbury J. M., Forrest M., Williams A. 1983; Mycoplasma lipofaciens, a new species of avian origin. Int. J. Syst. Bacteriol. 33:329–335
    [Google Scholar]
  11. Buntz B. 1987; Isolement et mise en evidence du pouvoir pathogene de M. gallisepticum chez l’oie (Anser anser). Ph.D. thesis. Universite de Bordeaux II, Villenave d’Ornon, France
    [Google Scholar]
  12. Buntz B., Bradbury J. M., Vuillaume A., Rousselot-Paillet D. 1986; Isolation of Mycoplasma gallisepticum from geese. Avian Pathol. 15:615–617
    [Google Scholar]
  13. Coie R. M. 1983; Transmission electron microscopy: basic techniques. Methods Mycoplasmol. 1:43–50
    [Google Scholar]
  14. Costas M., Leach R. H., Mitchelmore D. L. 1987; Numerical analysis of PAGE protein patterns and the taxonomic relationships within the “Mycoplasma mycoides cluster.”. J. Gen. Microbiol. 133:3319–3329
    [Google Scholar]
  15. Dove P., Bencina D., Antes R., Mann W. 1992; Recombinant DNA probes and PCR for detection of Mycoplasma gallisepticum strains. Int. Org. Mycoplasmol. Lett. 2:109
    [Google Scholar]
  16. Dupiellet J.-P. 1984; Mycoplasmes et acholeplasmes des palmipedes a foie gras: isolement, caracterisation, etude du role dans la pathologie. Rapport de D.E.A. de Pathologie. Universite de Bordeaux II, Villenave d’Ornon; France:
    [Google Scholar]
  17. Dupiellet J.-P. 1988; Mycoplasmes de l’oie et du canard: contribution a l’etude serologique et moleculaire de souches aparentees a Mycoplasma gallisepticum. Ph.D. thesis. Universite de Bordeaux II, Villenave d’Ornon, France
    [Google Scholar]
  18. Dupiellet J.-P., Vuillaume A., Rousselot D., Bove J. M., Bradbury J. M. 1988; Serological and molecular studies on Mycoplasma gallisepticum strains, p. 150. Abstr. 7th Congr. Int. Org. Mycoplasmol. International Organization for Myco-plasmology
    [Google Scholar]
  19. Dupiellet J. P., Vuillaume A., Rousselot D., Bove J. M., Bradbuiy J. M. 1990; Serological and molecular studies on Mycoplasma gallisepticum strains. Zentralbl. Bakteriol. Suppl. 20:859–864
    [Google Scholar]
  20. Edward D. G. ff., Moore W. B. 1975; A method for determining utilization of glucose by mycoplasmas. J. Med. Microbiol. 8:451–454
    [Google Scholar]
  21. Erno H., Stipkovits L. 1973; Bovine mycoplasmas. Cultural and biochemical studies. I. Acta Vet. Scand. 19:436–449
    [Google Scholar]
  22. Fernandez C., Mattsson J. G., Bolske G., Levisohn S., Johansson K.-E. Species-specific oligonucleotide probes complementary to 16S rRNA of Mycoplasma gallisepticum and Mycoplasma synoviae. Res. Vet. Sci. in press
    [Google Scholar]
  23. Freundt E. A., Andrews B. E., Erno H., Kunze M., Black F. T. 1973; The sensitivity of Mycoplasmatales to sodium polyanetholsulfonate and digitonin. Zentralbl. Bakteriol. Para-sitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 255:104–112
    [Google Scholar]
  24. Freundt E. A., Erno H., Lemcke R. M. 1979; Identification of mycoplasmas. Methods Microbiol. 13:377–434
    [Google Scholar]
  25. Gardella R. S., DelGiudice R. A. 1983; Hemagglutination, hemadsorption and hemolysis. Methods Mycoplasmol. 1:379–384
    [Google Scholar]
  26. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of MoUicutes 1979; Proposal of minimal standards for descriptions of new species of the class Molli-cutes. Int. J. Syst. Bacteriol. 29:172–180
    [Google Scholar]
  27. Johnson J. L. 1984; Bacterial classification. III. Nucleic acids in bacterial classification. p. 8–11 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams and Wilkins; Baltimore:
    [Google Scholar]
  28. Jordan F. T. W., Amin M. M. 1980; A survey of mycoplasma infections in domestic poultry. Res. Vet. Sci. 28:96–100
    [Google Scholar]
  29. Kleven S. H., Browning G. F., Bulach D. M., Ghiocas E., Morrow C. J., Whithear K. G. 1988; Examination of Mycoplasma gallisepticum strains using restriction endonuclease DNA analysis and DNA-DNA hybridization. Avian Pathol. 17:559–570
    [Google Scholar]
  30. Kleven S. H., Morrow C. J., Whithear K. G. 1988; Comparison of Mycoplasma gallisepticum strains by hemagglu- tination-inhibition and restriction enzyme analysis. Avian Dis. 32:731–741
    [Google Scholar]
  31. Livingston C. W. 1972; Isolation of T-strain mycoplasma from Texas feedlot cattle. Am. J. Vet. Res. 33:1925–1929
    [Google Scholar]
  32. Madden D. L., Henderson W. H., Moses H. E. 1967; Case report: isolation of Mycoplasma gallisepticum from bobwhite quail (Colinus virginianus). Avian Dis. 11:378–380
    [Google Scholar]
  33. Osborn O. H., Pomeroy B. S. 1958; Isolation of the agent of infectious sinusitis of turkeys from naturally infected pheasants. Avian Dis. 2:370–372
    [Google Scholar]
  34. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol. 102:306–310
    [Google Scholar]
  35. Reece R. L., Ireland L., Barr D. A. 1986; Infectious sinusitis associated with Mycoplasma gallisepticum in gamebirds. Aust. Vet. J. 63:167–168
    [Google Scholar]
  36. Rosendal S., Black F. T. 1972; Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies. Acta Pathol. Microbiol. Scand. Sect. B 80:615–622
    [Google Scholar]
  37. Saed O. M., Bradbury J. M. Unpublished data
  38. Saed O. M., Ross G., Bradbury J. M. 1990; Pathogenicity studies on an avian mycoplasma related to Mycoplasma gallisepticum. Int. Org. Mycoplasmol. Lett. 1:547–548
    [Google Scholar]
  39. Shepard M. C., Howard D. R. 1970; Identification of “T” mycoplasmas in primary agar cultures by means of a direct test for urease. Ann. N.Y. Acad. Sci. 174:809–819
    [Google Scholar]
  40. Tiong S. K. 1978; Isolation of Mycoplasma gallisepticum from sinuses of three quail (Cortumix cortunix japonica). Vet. Rec. 103:539
    [Google Scholar]
  41. Tully J. G., Whitcomb R. G., Clark H. F., Williamson D. L. 1977; Pathogenic mycoplasmas: cultivation and vertebrate pathogenicity of a new spiroplasma. Science 195:892–894
    [Google Scholar]
  42. Van Roekel H., Olesiuk O. M. 1953; The etiology of chronic respiratory disease. p. 289–303 In Proceedings of the 90th Annual Meeting of the American Veterinary Medicine Association American Veterinary Medical Association; Chicago:
    [Google Scholar]
  43. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., £. Murray R. G., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  44. Wichmann R. W. 1957; Case report—PPLO infection in chukar partridges (Alectoris graeca). Avian Dis. 1:222–226
    [Google Scholar]
  45. Yagihashi T., Nunoya T., Tajima M. 1988; Pathogenicity for chickens of six strains of Mycoplasma gallisepticum isolated from various birds. Avian Pathol. 17:725–729
    [Google Scholar]
  46. Yoder H. W. Jr., Hofstad M. S. 1964; Characterisation of avian mycoplasmas. Avian Dis. 8:481–512
    [Google Scholar]
  47. Yogev D., Levisohn S., Kleven S. H., Halachmi D., Razin S. 1988; Ribosomal RNA gene probes to detect intraspecies heterogeneity in Mycoplasma gallisepticum and M. synoviae. Avian Dis. 32:220–231
    [Google Scholar]
  48. Yogev D., Sela S., Bercovier H., Razin S. 1988; Elongation factor (EF-Tu) gene probe detects polymorphism in Mycoplasma strains. FEMS Microbiol. Lett. 50:145–149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-4-721
Loading
/content/journal/ijsem/10.1099/00207713-43-4-721
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error