1887

Abstract

We isolated a methanogenic strain, designated strain SA (= DSM 7056), from an enrichment culture inoculated with a Japanese paddy field soil. Cells of this strain were strictly anaerobic, nonmotile, short rods and stained gram positive. The strain was able to use H-COor formate as a methanogenic substrate. It required vitamins, but not acetate, for growth. Growth was fastest at 35 to 40°C. Methane was produced most rapidly at pH 6.0 to 7.5. The cellular lipid composition of strain SA was similar to that of A2 (= DSM 2462). The G+C content of the DNA was 26.4 mol%. Strain SA had DNA-DNA hybridization values of more than 70% with DH1(= DSM 1125). On the basis of phenotypic and genotypic characteristics, we identified strain SA as In the course of our identification work, the genetic heterogeneity of was revealed by the results of DNA-DNA hybridization experiments. Although strain AZ (= DSM 744) should be classified as a member of a species distinct from the species containing the other four strains studied (DH1, A2, DC [= DSM 1536], and SA), further phenotypic characterization will be required before a new species can be proposed.

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1993-10-01
2024-04-19
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References

  1. Akagawa-Matsushita M., Matsuo M., Koga Y., Yama-sato K. 1992; Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides. Int. J. Syst. Bacteriol. 42:621–627
    [Google Scholar]
  2. Batch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43:260–296
    [Google Scholar]
  3. Boone D. R., Whitman W. B. 1988; Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int. J. Syst. Bacteriol. 38:212–219
    [Google Scholar]
  4. Bouwman A. F. 1989; The role of soils and land use in the greenhouse effect. Neth. J. Agric. Sci. 37:13–19
    [Google Scholar]
  5. Conrad R., Bak F., Seitz H. J., Thebrath B., Mayer H. P., Schiitz H. 1989; Hydrogen turnover by psychrotrophic homoac- etogenic and mesophilic methanogenic bacteria in anoxic paddy soil and lake sediment. FEMS Microbiol. Ecol. 62:285–294
    [Google Scholar]
  6. Conway de Macario E., Macario A. J. L., Wolin M. J. 1982; Antigenic analysis of Methanomicrobiales and Methano brevibacter arboriphilus. J. Bacteriol. 152:762–764
    [Google Scholar]
  7. Doddema H. J., Derksen J. W. M., Vogels G. D. 1979; Fimbriae and flagella of methanogenic bacteria. FEMS Microbiol. Lett. 5:135–138
    [Google Scholar]
  8. Japanese Society of Soil Microbiology 1975 Experimental method in soil microbiology p. 431–443 Yokendo; Tokyo: (In Japanese.)
    [Google Scholar]
  9. Koga Y., Ohga M., Nishihara M., Morii H. 1987; Distribution of a diphytanyl ether analog of phosphatidylserine and an ethanolamine-containing tetraether lipid in methanogenic bacteria. Syst. Appl. Microbiol. 9:176–182
    [Google Scholar]
  10. Marmiir J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  11. Miller T. L. 1989; Genus II. Methanobrevibacter. p. 2178–2183 In Staley J. T., Bryant M. P., Pfennig N., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 3 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  12. Morii H., Koga Y. 1992; An improved assay method for a pseudomurein-degrading enzyme of Methanobacterium wolfei and the protoplast formation of Methanobacterium thermoau-totrophicum by the enzyme. J. Ferment. Bioeng. 73:6–10
    [Google Scholar]
  13. Morii H., Nishihara M., Koga Y. 1983; Isolation, characterization and physiology of a new formate-assimilable methanogenic strain (A2) of Methanobrevibacter arboriphilus. Agric. Biol. Chem. 47:2781–2789
    [Google Scholar]
  14. Morii H., Nishihara M., Koga Y. 1988; Composition of polar lipids of Methanobrevibacter arboriphilicus and structure determination of the signature phosphoglycolipid of Methano-bacteriaceae. Agric. Biol. Chem. 52:3149–3156
    [Google Scholar]
  15. Nishihara M., Koga Y. 1987; Extraction and composition of polar lipids from the archaebacterium, Methanobacterium thermoautotrophicum: effective extraction of tetraether lipids by an acidified solvent. J. Biochem. 101:997–1005
    [Google Scholar]
  16. Nishihara M., Koga Y. 1988; Quantitative conversion of diether or tetraether phospholipids to glycerophosphoesters by dealkylation with boron trichloride: a tool for structural analysis of archaebacterial lipids. J. Lipid Res. 29:384–388
    [Google Scholar]
  17. Nishihara M., Morii H., Koga Y. 1987; Structure determination of a quartet of novel tetraether lipids from Methanobacterium thermoautotrophicum. J. Biochem. 101:1007–1015
    [Google Scholar]
  18. Rajagopal B. S., Belay N., Daniels L. 1988; Isolation and characterization of methanogenic bacteria from rice paddies. FEMS Microbiol. Ecol. 53:153–158
    [Google Scholar]
  19. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  21. Zehnder A. J. B., Wuhrmann K. 1977; Physiology of a Methanobacterium strain AZ. Arch. Microbiol. 111:199–205
    [Google Scholar]
  22. Zeikus J. G. 1977; The biology of methanogenic bacteria. Bacteriol. Rev. 41:514–541
    [Google Scholar]
  23. Zeikus J. G., Henning D. L. 1975; Methanobacterium arbophilicum sp. nov. An obligate anaerobe isolated from wetwood of living trees. Antonie Leeuwenhoek 41:543–552
    [Google Scholar]
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