1887

Abstract

A test is described that is useful for characterizing mollicutes in terms of the ability to maintain growth in medium containing 15 to 20% fetal bovine serum or in serum-free media with or without 0.04% Tween 80 (polyoxyethylene sorbitan). Representative species maintained growth in serum-free medium, and about half of the strains tested grew well in Tween 80-supplemented medium. Representative and species did not maintain growth in either serum-free medium alone or when Tween 80 was added. species and group representatives generally failed to sustain growth in serum-free medium with or without Tween 80, but at least four of the spiroplasmas tested maintained growth in serum-free medium. The representative species grew in serum-free media only when Tween 80 was added, as did . Although the test has obvious determinative uses for members of the class , it does not supplant the conventional methodology for assaying the cholesterol requirements of these organisms.

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1993-07-01
2022-05-19
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References

  1. Bonnet F., Saillard C., Vignault J.-C., Garnier M., Carle P., Bove J. M., Rose D. L., Tully J. G., Whitcomb R. F. 1991; Acholeplasma seiffertii, sp. nov., a mollicute from plant surfaces.. Int. J. Syst. Bacteriol 41:45–49
    [Google Scholar]
  2. Davis R. E., Lee I.-M., Worley J. F. 1981; Spiroplasma floricola a new species isolated from surfaces of flowers of the tulip tree, Liriodendron tulipifera L. Int. J. Syst. Bacteriol 31:456–164
    [Google Scholar]
  3. Eden-Green S. J., Tully J. G. 1979; Isolation of Acholeplasma spp. from coconut palms affected by lethal yellowing disease in Jamaica. Curr. Microbiol 2:311–316
    [Google Scholar]
  4. Edward D. G. ff. 1971; Determination of sterol requirement for Mycoplasmatales.. J. Gen. Microbiol 69:205–210
    [Google Scholar]
  5. Guo Y. H., Chen T. A., Whitcomb R. F., Rose D. L., Tully J. G., Williamson D. L., Ye X. D., Chen Y. X. 1990; Spiroplasma chinense sp. nov. from flowers of Cafystegia hederacea in China. Int. J. Syst. Bacteriol 40:421–425
    [Google Scholar]
  6. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes. 1979; Proposal of minimum standards for the description of new species of the class Mollicutes. Int. J. Syst. Bacteriol 29:172–180
    [Google Scholar]
  7. McCoy R. E., Basham H. G., Tully J. G., Rose D. L., Carle P., Bove J. M. 1984; Acholeplasma florum: a new species isolated from plants. Int. J. Syst. Bacteriol 34:11–15
    [Google Scholar]
  8. Navas-Castillo J., Laigret F., Tully J. G., Bove J. M. 1992; Le mollicute Acholeplasma florum possede un gene du systeme phosphoenolpyruvate sucre-phosphotransferase et il utilise UGA comme codon tryptophan. C.R. Acad. Sci. Ser. Ill Sci. Vie 315:43–48
    [Google Scholar]
  9. Neimark H. C., Lange C. S. 1990; Pulse-field electrophoresis indicates full-length mycoplasma chromosomes range widely in size. Nucleic Acids Res 18:5443–5448
    [Google Scholar]
  10. Neimark H. C., Tully J. G., Rose D., Lange C. 1992; Chromosome size polymorphism among mollicutes. Proc. 9th Int. Congr. Internal Organ Mycoplasmol. IOM Lett 2:261
    [Google Scholar]
  11. Nes W. D. 1987; Biosynthesis and requirement for sterols in the growth and reproduction of oomycetes. Am. Chem. Soc. Symp. Ser 325:305–312
    [Google Scholar]
  12. Oliver J. E., Rose D. L., Tully J. G., Whitcomb R. F. Unpublished data
  13. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol 102:306–310
    [Google Scholar]
  14. Robertson J. A., Pyle L. E., Stemke G. W., Finch L. R. 1990; Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis. Nucleic Acids Res 18:1451–1456
    [Google Scholar]
  15. Robinson I. M., Freundt E. A. 1987; Proposal for an amended classification of anaerobic mollicutes. Int. J. Syst. Bacteriol 37:78–81
    [Google Scholar]
  16. Rose D. L., Kocka J. P., Somerson N. L., Tully J. G., Whitcomb R. F., Carle P., Bove J. M., Colflesh D. E., Williamson D. L. 1990; Mycoplasma lactucae sp. nov. a sterol-requiring mollicute from a plant surface. Int. J. Syst. Bacteriol 40:138–142
    [Google Scholar]
  17. Tully J. G. 1979; Special features of the acholeplasmas.. p. 431–449 In Barile M. F., Razin S. (ed.) The mycoplasmas vol. 1: Academic Press Inc.; New York:
    [Google Scholar]
  18. Tully J. G. 1983; Cloning and filtration techniques for mycoplasmas. Methods Mycoplasmol 1:173–177
    [Google Scholar]
  19. Tully J. G. 1983; Tests for digitonin sensitivity and sterol requirement. Methods Mycoplasmol 1:355–362
    [Google Scholar]
  20. Tully J. G. 1983; Reflections on recovery of some fastidious mollicutes with implications of the changing host patterns of these organisms. Yale J. Biol. Med 56:799–813
    [Google Scholar]
  21. Tully J. G. 1984; Family Acholeplasmataceae genus Acholeplasma. p. 781–787 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology vol. 1: The Williams … ;Wilkins Co.; Baltimore:
    [Google Scholar]
  22. Tully J. G., Bove J. M., Laigret F., Whitcomb R. F. 1993; Revised taxonomy of the class Mollicutes’. proposed elevation of a monophyletic cluster of arthropod-associated mollicutes to ordinal rank (Entomoplasmatales ord. nov.) with provision for familial rank to separate species with nonhelical morphology (Entomoplasmataceae, fam. nov.) from helical species (Spiro-plasmataceae) and emended descriptions of the order Myco-plasmatales, family Mycoplasmataceae. Int. J. Syst. Bacteriol 43:378–385
    [Google Scholar]
  23. Tully J. G., Razin S. 1970; Acholeplasma axanthum sp. n.: a new sterol-nonrequiring member of the Mycoplasmatales. J. Bacteriol 103:751–754
    [Google Scholar]
  24. Tully J. G., Rose D. L., Carle P., Bove J. M., Hackett K. J., Whitcomb R. F. 1988; Acholeplasma entomophilum sp. nov. from gut contents of a wide range of host insects. Int. J. Syst. Bacteriol 38:164–167
    [Google Scholar]
  25. Tully J. G., Rose D. L., Whitcomb R. F., Hackett K. J., Clark T. B., Henegar R. B., Clark E., Carle P., Bove J. M. 1987; Characterization of some new insect-derived acholeplasmas. Isr. J. Med. Sci 23:699–703
    [Google Scholar]
  26. Tully J. G., Taylor-Robinson D., Rose D. L., Cole R. M., Bove J. M. 1983; Mycoplasma genitalium a new species from the human urogenital tract. Int. J. Syst. Bacteriol 33:387–396
    [Google Scholar]
  27. Tully J. G., Whitcomb R. F., Rose D. L., Hackett K. J., Clark E., Henegar R. B., Carle P., Bove J. M. 1990; Current insight into the host diversity of acholeplasmas. Zentralbl. Bakteriol. Suppl 20:461–467
    [Google Scholar]
  28. Weisburg W., Tully G. J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of mycoplasmas: basis for their classification. J. Bacteriol 171:6455–6457
    [Google Scholar]
  29. Whitcomb R. F. 1983; Culture media for spiroplasmas. Methods Mycoplasmol 1:147–158
    [Google Scholar]
  30. Williamson D. L., Tully J. G., Rose D. L., Hackett K. J., Henegar R., Carle P., Bove J. M., Colflesh D. E., Whitcomb R. F. 1990; Mycoplasma somnilux sp. nov. Mycoplasma luminosum sp. nov., and Mycoplasma lucivorax sp. nov., new sterol-requiring mollicutes from firefly beetles (Coleoptera: Lampyridae). Int. J. Syst. Bacteriol 40:160–164
    [Google Scholar]
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