1887

Abstract

We propose the name sp. nov. for a rickettsial serotype of unknown pathogenicity isolated in 1979 in Switzerland from ticks and designated the Swiss agent. The growth characteristics and the results of microimmunofluorescence serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting (immunoblotting) with specific mice sera, and a polymerase chain reaction followed by restriction fragment length polymorphism analysis confirmed previously reported preliminary findings which suggested that this rickettsia, to which a name was given provisionally, does represent a new member of the spotted fever group of rickettsiae. The type strain is C3 (Reference Center for Rickettsioses, Marseille, France).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-3-521
1993-07-01
2022-08-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/3/ijs-43-3-521.html?itemId=/content/journal/ijsem/10.1099/00207713-43-3-521&mimeType=html&fmt=ahah

References

  1. Anacker R. L., Philip R. N., Casper E., Todd W. J., Mann R. E., Johnston M. R., Nauck C. J. 1983; Biological properties of rabbit antibodies to a surface antigen of Rickettsia rickettsii. Infect. Immun 40:292–298
    [Google Scholar]
  2. Beati L., Eremeeva M., Yu X. J., Tissot Dupont H., Raoult D. 1992; Rickettsia massiliae (sp. nov.). Abstr. 10th Ses-quiannu. Meet. Am. Soc Rickettsiol; Rickettsial Dis:
    [Google Scholar]
  3. Beati L., Finidori J.-P., Gilot B., Raoult D. 1992; Comparison of microimmunofluorescence serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and polymerase chain reaction followed by restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J. Clin. Microbiol 30:1922–1930
    [Google Scholar]
  4. Burgdorfer W., Aeschlimann A., Peter O., Hayes S. F., Philip R. N. 1979; Ixodes ricinus vector of a hitherto undescribed spotted fever group agent in Switzerland. Acta Trop 36:357–367
    [Google Scholar]
  5. Fiset P., Ormsbee R. A., Silbermann R., Peacock M., Spielman S. H. 1969; A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol 13:60
    [Google Scholar]
  6. Gimenez D. F. 1964; Staining rickettsiae in yolk-sac cultures. Stain Technol 39:135–140
    [Google Scholar]
  7. Goldwasser R. A., Steinman Y., Klingberg W., Swartz T. A., Klingberg M. A. 1974; The isolation of strains of rickettsiae of the spotted fever group in Israel and their differentiation from other members of the group by immunofluorescence methods. Scand. J. Infect. Dis 6:53–62
    [Google Scholar]
  8. Hayes S. F., Burgdorfer W., Aeschlimann A. 1980; Sexual transmission of spotted fever group rickettsiae by infected male ticks: detection of rickettsiae in immature spermatozoa of Ixodes ricinus. Infect. Immun 27:638–642
    [Google Scholar]
  9. Kelly P. J., Matthewman L. A., Beati L., Raoult D., Mason P. R., Dreary M., Makombe R. 1992; African tick-bite fever: a new spotted fever group rickettsiosis under an old name. Lancet 340:982–983
    [Google Scholar]
  10. Kelly P. J., Raoult D., Mason P. R. 1991; Isolation of spotted fever group rickettsias from triturated ticks using a modification of the centrifugation-shell vial technique. Trans. R. Soc. Trop. Med. Hyg 85:397–398
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  12. Peter O. 1981; Contribution a la connaissance de Pecologie de la “rickettsie suisse” transmise par la tique Ixodes ricinus L. 1758. Ph.D. thesis. Faculty of Sciences University of Neuchatel; Neuchatel, Switzerland:
    [Google Scholar]
  13. Peter O. 1985; Presence d’anticorps contre la “rickettsie suisse” chez les mammiferes sauvages et domestiques du Canton de Neuchatel. Schweiz. Arch. Tierheilkd 127:461–468
    [Google Scholar]
  14. Peter O., Raoult D., Gilot B. 1990; Isolation by sensitive centrifugation cell culture system of 52 strains of spotted fever group rickettsiae from ticks collected in France. J. Clin. Microbiol 28:1597–1599
    [Google Scholar]
  15. Peter O., Burgdorfer J. 1985; Rickettsia helvetica, a new spotted fever group rickettsia: immunochemical analysis of the antigens of 5 spotted fever group rickettsiae. p. 99–105 In Kazar J. (ed.) Rickettsiae and rickettsial diseases Slovak Academy of Sciences; Bratislava, Czechoslovakia:
    [Google Scholar]
  16. Philip R. N., Casper E. A., Burgdorfer W., Gerloff R. K., Hughes L. E., Bell E. J. 1978; Serologic typing of rickettsiae of the spotted fever group by indirect microimmunofluorescence. J. Immunol 121:1961–1968
    [Google Scholar]
  17. Regneiy R. L., Spruill C. L., Plikaytis B. D. 1991; Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J. Bacteriol 173:1576–1589
    [Google Scholar]
  18. Robertson R. G., Wissemann C. L. 1973; Tick-borne rickettsiae of the spotted fever group in West Pakistan. Am. J. Epidemiol 97:55–64
    [Google Scholar]
  19. Teysseire N., Chiche-Portiche C., Raoult D. 1992; Intracellular movements of Rickettsia conorii and Rickettsia typhi based on actin polymerization. Res. Microbiol 143:821–829
    [Google Scholar]
  20. Towbin H., Staehelin T., Gordo J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354
    [Google Scholar]
  21. Webb L., Carl M., Malloy D. M., Dasch G. A., Azad A. F. 1990; Detection of murine typhus infection in fleas using polymerase chain reaction. J. Clin. Microbiol 28:530–534
    [Google Scholar]
  22. Weiss E., Coolbaugh J. C., Williams J. C. 1975; Separation of viable Rickettsia typhi from yolk sac and L-cell host components by Renografin density gradient centrifugation. Appl. Microbiol 30:456–463
    [Google Scholar]
  23. Yu X., H- Walker D., Jerrells T. R. 1990; Polypeptides constituting the antigenic basis for identification of Rickettsia sibirica species by the standard serotyping method for spotted fever group rickettsiae. Acta Virol 34:71–79
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-3-521
Loading
/content/journal/ijsem/10.1099/00207713-43-3-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error