Serovar Determination and Molecular Taxonomic Correlation in , and : A Cooperative Study of the International Working Group on Mycobacterial Taxonomy Free

Abstract

A cooperative study was conducted by the International Working Group on Mycobacterial Taxonomy to correlate the agglutination serovar designations of , and strains with the species ascriptions of these organisms according to molecular criteria and cultural properties and to assess the reproducibility of serovar determinations for a set of 63 reference strains of these species. Among the molecular criteria, the level of agreement between results obtained with nucleic acid probes and T-catalase serology results was 94% for strains of and . Nucleic acid probes were not available for , but none of the 10 strains ascribed to this species on the basis of catalase serology data reacted with a nucleic acid probe for or . Ascription to a species on the basis of mycolic acid high-performance liquid chromatography patterns was in agreement with catalase serology results in 86% of the cases examined. Most strains belonging to serovars 1 through 6 and 8 through 11 were identified by molecular criteria as , most strains belonging to serovars 7, 12 through 20, 23, and 25 were identified as , and most strains belonging to serovars 41 through 43 were identified as , in agreement with common current practice. Evidence for assigning serovar 27 to was obtained. However, two strains of a given serovar may, on occasion, be placed in different species. The dominant species assignments for strains belonging to serovars 21, 24, 26, and 28 remain unresolved. Data from laboratories which used panels of sera that corresponded to all of the serovars represented in the study were in agreement with the consensus results in 84% of the instances. Laboratories in which a limited panel of sera was used were far more likely to report results that did not agree with the consensus results.

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1993-07-01
2024-03-29
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References

  1. Baess I. 1983; Deoxyribonucleic acid relationships between different serovars of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum.. Acta Pathol. Microbiol. Immunol. Scand 91:201–203
    [Google Scholar]
  2. Brennan P. J., Mayer H., Aspinall G. O., Nam Shin J. E. 1981; Structures of the glycopeptidolipid antigens from serovars in the Mycobacterium avium/Mycobacterium intracellulare /Mycobacterium scrofulaceum. serocomplex. Eur. J. Biochem 115:7–15.
    [Google Scholar]
  3. Butler W. R., Jost K. C. Jr., Kilbum J. O. 1991; Identification of mycobacteria by high-performance liquid chromatography. J. Clin. Microbiol 29:2468–2472
    [Google Scholar]
  4. Butler W. R., Kilbum J. O. 1988; Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids. J. Clin. Microbiol 26:50–53
    [Google Scholar]
  5. Denner J. C., Tsang A. Y., Chatterjee D., Brennan P. J. 1992; Comprehensive approach to identification of serovars of Mycobacterium avium complex. J. Clin. Microbiol 30:473–478
    [Google Scholar]
  6. Ferguson C., Hammond P., Lankford R., McAllister D., Jonas V. 1991; Phenotypic discordance within species of the genus Mycobacterium revealed by application of DNA probes abstr. 30. Progr. Abstr. 91st Annu. Meet. Am. Soc. Microbiol 1991 American Society for Microbiology; Washington D.C.:
    [Google Scholar]
  7. Good R. C., Beam R. 1984; Seroagglutination. p. 105–122 In Kubica G. P., Wayne L. G. (ed.) The mycobacteria: a sourcebook Marcel Dekker Inc.; New York:
    [Google Scholar]
  8. Goodfellow M., Lind A., Mordarska H., Pattyn S., Tsukamura M. 1974; A co-operative numerical analysis of cultures considered to belong to the “rhodochrous” taxon. J. Gen. Microbiol 85:291–302
    [Google Scholar]
  9. Goslee S., Rynearson T. K., Wolinsky E. 1976; Additional serotypes of Mycobacterium scrofulaceum Mycobacterium gordonae Mycobacterium marinum, and Mycobacterium xe-nopi determined by agglutination. Int. J. Syst. Bacteriol 26:136–142
    [Google Scholar]
  10. Kubica G. P., Baess I., Gordon R. E., Jenkins P. A., Kwapinski J. B. G., McDurmont C., Pattyn S. R., Saito H., Silcox V. A., Stanford J. L., Takeya K., Tsukamura M. 1972; A cooperative numerical analysis of rapidly growing mycobacteria. J. Gen. Microbiol 73:55–70
    [Google Scholar]
  11. Lim S. D., Todd J., Lopez J., Ford E., Janda J. M. 1991; Genotypic identification of pathogenic Mycobacterium species by using a nonradioactive oligonucleotide probe. J. Clin. Microbiol. 29:1276–1278
    [Google Scholar]
  12. Meissner G., Schroder K. H., Amadio G. E., Anz W., Chaparas S., Engel H. W. B., Jenkins P. A., Kappler W., Kleeberg H. H., Kubala E., Kubin M., Lauterbach D., Lind A., Magnusson M., Mikova Z. D., Pattyn S. R., Schaefer W. B., Stanford J. L., Tsukamura M., Wayne L. G., Willers I., Wolinsky E. 1974; A cooperative numerical analysis of nonscoto-and nonphotochromogenic slowly growing mycobacteria. J. Gen. Microbiol 83:207–235
    [Google Scholar]
  13. Reznikov M., Leggo J. H. 1972; Modification of Schaefer’s procedure for serotyping of organisms of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex. Appl. Microbiol 23819–823
    [Google Scholar]
  14. Saito H., Gordon R. E., Juhlin I., Kappler W., Kwapinski J. B. G., McDurmont C., Pattyn S. R., Runyon E. H., Stanford J. L., Tarnok I., Tasaka H., Tsukamura M., Weiszfeiier J. 1977; Cooperative numerical analysis of rapidly growing mycobacteria. Int. J. Syst. Bacteriol 27:75–85
    [Google Scholar]
  15. Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. 1990; Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare.. J. Clin. Microbiol 28:1694–1697
    [Google Scholar]
  16. Schaefer W. B. 1965; Serologic identification and classification of atypical mycobacteria by their agglutination. Am. Rev. Respir. Dis 92:Suppl.85–93
    [Google Scholar]
  17. Tsang A. Y., Denner J. C., Brennan P. J., McClatchy J. K. 1992; Clinical and epidemiological importance of typing of Mycobacterium avium complex isolates. J. Clin. Microbiol 30:479–484
    [Google Scholar]
  18. Wayne G. L., Andrade L., Froman S., Kappler W., Kubala E., Meissner G., Tsukamura M. 1979; A co-operative numerical analysis of Mycobacterium gastri Mycobacterium kansasii and Mycobacterium marinum. J. Gen. Microbiol 109:319–327
    [Google Scholar]
  19. Wayne L. G., Diaz G. A. 1986; Differentiation between T-catalases derived from Mycobacterium avium and Mycobacterium intracellulare by a solid-phase immunosorbent assay. Int. J. Syst. Bacteriol 36:363–367
    [Google Scholar]
  20. Wayne L. G., Diaz G. A. 1987; Intrinsic catalase dot blot immunoassay for identification of Mycobacterium tuberculosis Mycobacterium avium, and Mycobacterium intracellulare.. J. Clin. Microbiol 251687–1690
    [Google Scholar]
  21. Wayne L. G., Dietz T. M., Gernez-Rieux C., Jenkins P. A., Kappler W., Kubica G. P. G., Kwapinski J. B., Meissner G., Pattyn S. R., Runyon E. H., Schroder K. H., Silcox V. A., Tsukamura M., Wolinsky E. 1971; A cooperative numerical taxonomic analysis of scotochromogenic slowly growing mycobacteria. J. Gen. Microbiol 66:255–271
    [Google Scholar]
  22. Wayne L. G., Engbaek H. C. B., Engel H. W., Froman S., Gross W., Hawkins J., Kappler W., Karlson A. G., Kleeberg H. H., Krasnow I., Kubica G. P., McDurmont C., Net E. E., Pattyn S. R., Schroder K. H., Showalter S., Tarnok I., Tsukamura M., Vergmann B., Wolinsky E. 1974; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium : tests for pigment urease resistance to sodium chloride hydrolysis of Tween 80 and (3-galactosidase. Int. J. Syst. Bacteriol 24:412–419
    [Google Scholar]
  23. Wayne L. G., Engel H. W. B., Grassi C., Gross W., Hawkins J., Jenkins P. A., Kappler W., Kleeberg H. H., Krasnow I., Nel E. E., Pattyn S. R., Richards P. A., Showalter S., Slosarek M., Szabo I., Tarnok I., Tsukamura M., Vergmann B., Wolinsky E. 1976; Highly reproducible techniques for use in systematic bacteriology in the genus Mycobacterium. II. Tests for niacin and catalase and for resistance to isoniazid thiophene 2-carbox-ylic acid hydrazide hydroxylamine and p-nitrobenzoate. Int. J. Syst. Bacteriol 26:311–318
    [Google Scholar]
  24. Wayne L. G., Good R. C., Krichevsky M. I., Beam R. E., Blacklock Z., Chaparas S. D., Dawson D., Froman S., Gross W., Hawkins J., Jenkins P. A., Juhlin I., Kappler W., Kleeberg H. H., Krasnow I., Lefford M. J., Mankiewicz E., McDurmont C., Meissner G., Morgan P., Nel E. E., Pattyn S. R., Portaels F., Richards P. A., Riisch S., Schroder K. H., Silcox V. A., Szabo I., Tsukamura M., Vergmann B. 1981; First report of the cooperative open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int. J. Syst. Bacteriol 31:1–20
    [Google Scholar]
  25. Wayne L. G., Good R. C., Krichevsky M. I., Beam R. E., Blacklock Z., David H. L., Dawson D., Gross W., Hawkins J., Jenkins P. A., Juhlin I., Kappler W., Kleeberg H. H., Krasnow I., Lefford M. J., Mankiewicz E., McDurmont C., Nel E. E., Portaels F., Richards P. A., Riisch S., Schroder K. H., Silcox V. A., Szabo I., Tsukamura M., Van den Breen L., Vergmann B. 1983; Second report of the cooperative open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int. J. Syst. Bacteriol 33:265–274
    [Google Scholar]
  26. Wayne L. G., Good R. C., Krichevsky M. I., Blacklock Z., David H. L., Dawson D., Gross W., Hawkins J., Jenkins P. A., Juhlin I., Kappler W., Klee berg H. H., Levy-Frebault V., McDurmont C., Nel E. E., Portaels Riisch-Gerdes F., Schroder K. H., Silcox V. A., Szabo I., Tsukamura M., Van Den Breen L., Vergmann B., Yakrus M. A. 1989; Third report of the cooperative open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int. J. Syst. Bacteriol 39:267–278
    [Google Scholar]
  27. Wayne L. G., Good R. C., Krichevsky M. I., Blacklock Z., David H. L., Dawson D., Gross W., Hawkins J., Levy-Frebault V. V., McManus C., Portaels F., Riisch-Gerdes S., Schroder K. H., Silcox V. A., Tsukamura M., Van Den Breen L., Yakrus M. A. 1991; Fourth report of the cooperative open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int. J. Syst. Bacteriol 41:463–472
    [Google Scholar]
  28. Wayne L. G., Hollander D., Anderson B., Sramek H. A., Vadheim C. M., Rotter J. I. 1992; Immunoglobulin A (IgA) and IgG serum antibodies to mycobacterial antigens in Crohn’s disease patients and their relatives. J. Clin. Microbiol 30:2013–2018
    [Google Scholar]
  29. Wolinsky E. 1979; Emendation of proposed additional serotypes of mycobacteria determined by agglutination. Int. J. Syst. Bacteriol 29:59
    [Google Scholar]
  30. Wolinsky E., Schaefer W. B. 1973; Proposed numbering scheme for mycobacterial serotypes. Int. J. Syst. Bacteriol 23:182–183
    [Google Scholar]
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