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Abstract

Abstract

By using specific primers, the 16S rRNA genes of Japanese mycoplasmalike organisms (MLOs) were amplified by polymerase chain reactions from MLO-enriched fractions of plants infected with each of six different MLOs. Each of the polymerase chain reaction fragments (length, 1,370 nucleotides) was directly sequenced in both strands by using 17 oligonucleotide primers. A phylogenetic tree constructed by using the sequence data showed that these Japanese MLOs are phylogenetically diverse microorganisms that fall into three groups, group I (onion yellows, tomato yellows, mulberry dwarf, and paulownia witches’ broom MLOs), group II (tsuwabuki witches’ broom MLO), and group III (rice yellow dwarf MLO). A high level of sequence homology (99%) between the MLO and the severe strain of the western aster yellows MLO on the one hand and group I MLOs on the other indicates that the MLO and the severe strain of the western aster yellows MLO belong to group I and suggests that these MLOs, isolated from two geographically separated locations, descended from a very similar ancestor. Although group I contains phylogenetically identical MLOs, the organisms are transmitted by diverse insect vectors. The three MLO groups are more closely related to than to Thus, although MLOs are phylogenetically diverse, they are evolutionarily distant from other mollicutes. These data, together with other information (including phylogenetic relationships, vector specificity, plant-pathogenic properties, and habitat in plant phloem sieve tubes), suggest that MLOs could be classified into at least three phylogenetic groups (groups I through III).

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1993-07-01
2024-12-09
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References

  1. Clark M. F., Morton A., Buss S. L. 1989; Preparation of mycoplasma immunogens from plants and a comparison of polyclonal associated antigens. Ann. Appl. Biol. 114:111–124
    [Google Scholar]
  2. Davis R. E., Dally E. L., Bertaccini A., Credi R., Lee I.-M., Osier R., Carraro L., Barba M. 1992; Cloned DNA probes for specific detection of Italian periwinkle virescence mycoplasmalike organism (MLO) and investigation of genetic relatedness with other MLOs. Phytopathol. Mediterr. 31:5–12
    [Google Scholar]
  3. Davis R. E., Sinclair W. A., Lee I.-M., Dally E. L. 1992; Cloned DNA probes specific for detection of a mycoplasmalike organism associated with ash yellows. Mol. Plant-Microbe Interact. 5:163–169
    [Google Scholar]
  4. Doi Y., Teranaka M., Yora K., Asuyama H. 1967; Mycoplasma or P.L.T. group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches’-broom, aster yellows or paulownia witches’-broom. Ann. Phytopathol. Soc. Jpn. 33:259–266
    [Google Scholar]
  5. Eden-Green S. J., Waters H. 1981; Isolation and preliminary characterization of a spiroplasma from coconut palms in Jamaica. J. Gen. Microbiol. 124:263–270
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  7. Innis M. A., Gelfand D. H. 1990; Optimization of PCRs. 3–12 Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. PCR protocols: a guide to methods and applications Academic Press; San Diego, Calif:
    [Google Scholar]
  8. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Mollicutes. 1993; Minutes of the interim meetings, 1 and 2 August 1992, Ames, Iowa. Int. J. Syst. Bacteriol. 43:394–397
    [Google Scholar]
  9. Kato S., Iwanami S. 1990; A new MLO disease, tsuwabuki witches’ broom in Nichinan. Ann. Phytopathol. Soc. Jpn. 56:394
    [Google Scholar]
  10. Kato S., Shiomi T., Wakibe H., Iwanami S. 1988; Tomato yellows transmitted by the leafhopper vector, Macrosteles orientalis Virbaste. Ann. Phytopathol. Soc. Jpn. 54:220–223
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111–120
    [Google Scholar]
  12. Kirkpatrick B. C., Stenger D. C., Morris T. J., Purcell A. H. 1987; Cloning and detection of DNA from a noncultur- able plant pathogenic mycoplasma-like organism. Science 238:197–200
    [Google Scholar]
  13. Kloepper J. W., Garrott D. G. 1980; Relation of in vivo morphology to isolation of plant spiroplasmas. Curr. Microbiol. 4:365–370
    [Google Scholar]
  14. Kollar A., Seemiiller E. 1989; Base composition of the DNA of mycoplasmalike organisms associated with various plant diseases. J. Phytopathol. 127:177–186
    [Google Scholar]
  15. Kollar A., Seemiiller E., Bonnet F., Saillard C., Bové J. M. 1990; Isolation of the DNA of various plant pathogenic mycoplasmalike organisms from infected plants. Phytopathology 80:233–237
    [Google Scholar]
  16. Kuske C. R., Kirkpatrick B. C. 1990; Identification and characterization of plasmids from the western aster yellows mycoplasmalike organism. J. Bacteriol. 172:1628–1633
    [Google Scholar]
  17. Kuske C. R., Kirkpatrick B. C. 1992; Phylogenetic relationships between the western aster yellows mycoplasmalike organism and other procaryotes established by 16S rRNA gene sequence. Int. J. Syst. Bacteriol. 42:226–233
    [Google Scholar]
  18. Lee I.-M., Davis R. E. 1986; Prospects for in vitro culture of plant-pathogenic mycoplasma-like organisms. Annu. Rev. Phytopathol. 24:339–354
    [Google Scholar]
  19. Lee I.-M., Davis R. E. 1992; Mycoplasmas which infect plants and insects. 379–390 Maniloff J., McElhaney R. N., Finch L. R., Baseman J. B. Mycoplasmas: molecular biology and pathogenesis American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  20. Lee I.-M., Davis R. E., Chen T.-A., Chiykowski L. N., Fletcher J., Hiruki C., Schaff D. A. 1992; A genotype-based system for identification and classification of mycoplasmalike organisms (MLOs) in the aster yellows MLO strain cluster. Phytopathology 82:977–986
    [Google Scholar]
  21. Lee I.-M., Davis R. E., DeWitt N. D. 1990; Nonradioactive screening method for isolation of disease-specific probes to diagnose plant diseases caused by mycoplasmalike organisms. Appl. Environ. Microbiol. 56:1471–1475
    [Google Scholar]
  22. Lee I.-M., Davis R. E., Hiruki C. 1991; Genetic interrelatedness among clover proliferation mycoplasmalike organisms (MLOs) and other MLOs investigated by nucleic acid hybridization and restriction fragment length polymorphism analyses. Appl. Environ. Microbiol. 57:3565–3569
    [Google Scholar]
  23. Lee I.-M., Gundersen D. E., Davis R. E., Chiykowski L. N. 1992; Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge. J. Bacteriol. 174:6694–6698
    [Google Scholar]
  24. Lim P.-O., Sears B. B. 1989; 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolution- arily distinct from animal mycoplasmas. J. Bacteriol. 171:5901–5906
    [Google Scholar]
  25. Lim P.-O., Sears B. B. 1991; The genome size of a plant-pathogenic mycoplasmalike organism resembles those of animal mycoplasmas. J. Bacteriol. 173:2128–2130
    [Google Scholar]
  26. Lim P.-O., Sears B. B. 1991; DNA sequence of the ribosomal protein genes rpl2 and rpsl9 from a plant-pathogenic mycoplasma-like organism. FEMS Microbiol. Lett. 84:71–74
    [Google Scholar]
  27. Lim P.-O., Sears B. B. 1992; Evolutionaiy relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J. Bacteriol. 174:2606–2611
    [Google Scholar]
  28. Lim P.-O., Sears B. B., Klomparens K. L. 1992; Membrane properties of a plant-pathogenic mycoplasmalike organism. J. Bacteriol. 174:682–686
    [Google Scholar]
  29. Lin C. P., Chen T. A. 1985; Monoclonal antibodies against the aster yellows agent. Science 227:1233–1235
    [Google Scholar]
  30. Maniloff J. 1983; Evolution of wall-less prokaryotes. Annu. Rev. Microbiol. 37:477–499
    [Google Scholar]
  31. McCoy, R. E., A. Caudwell, C. J. Chang, T. A. Chen, L. N. Chiykowski, M. T. Cousin, J. L. Dale, G. T. N. de Leeuw, D. A. Golino, K. J. Hackett, B. C. Kirkpatrick, R. Marwitz, H. Petzold, R. C. Sinha, M. Sugiura, R. F. Whitcomb, I. L. Yang, B. M. Zhu Seemüller E. 1989; Plant diseases associated with mycoplasma-like organisms. 545–640 Whitcomb R. F., Tully J. G. The mycoplasmas, vol. 5. Spiroplasmas, acholeplasmas, and mycoplasmas of plants and arthropods Academic Press; New York:
    [Google Scholar]
  32. Miyahara K., Matsuzaki M., Tanaka K., Sako N. 1982; A new disease of onion caused by mycoplasma-like organism in Japan. Ann. Phytopathol. Soc. Jpn. 48:551–554
    [Google Scholar]
  33. Namba S. Unpublished data
    [Google Scholar]
  34. Namba S., Kato S., Iwanami S., Oyaizu H., Shiozawa H., @@ @@, Tsuchizaki T. Detection and differentiation of plant-pathogenic mycoplasma-like organisms using polymerase chain reaction Phytopathology, in press;
    [Google Scholar]
  35. Nasu S., Sugiura M., Wakimoto S., Iida T. 1967; On the etiologic agent of rice yellow dwarf. Ann. Phytopathol. Soc. Jpn. 33:343–344
    [Google Scholar]
  36. Neefs J. M., van de Peer Y., Hendriks L., De Wachter R. 1990; Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 18:Sequence Suppl.2237–2317
    [Google Scholar]
  37. Neimark H. C., Lange C. S. 1990; Pulsed-field electrophoresis indicates full-length mycoplasma chromosomes range widely in size. Nucleic Acids Res. 18:5443–5448
    [Google Scholar]
  38. Nyland G., Raju B. C. 1978; Isolation and culture of a spiroplasma from pear trees affected by pear decline. Phytopathol. News 12:216
    [Google Scholar]
  39. Raju B. C., Nyland G. 1978; Effects of different media on the growth and morphology of three newly isolated plant spiroplasmas. Phytopathol. News 12:216
    [Google Scholar]
  40. Razin S. 1985; Molecular biology and genetics of mycoplasmas (Mollicutes). Microbiol. Rev. 49:419–455
    [Google Scholar]
  41. Rogers M. J., Simmons J., Walker R. T., Weisburg W. G., Woese D. R., Tanner R. S., Robinson I. M., Stahl D. A., Olsen G., Leach R. H., Maniloff J. 1985; Construction of the mycoplasma evolutionary tree from 5S rRNA sequence data. Proc. Natl. Acad. Sci. USA 82:1160–1164
    [Google Scholar]
  42. Saiki R. K., Gelfand D. H., Stoffe S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primerdirected enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  43. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406–425
    [Google Scholar]
  44. Sambrook J., Fritsch E. F., Maniatis T. 1982 Molecular cloning: a laboratory manual, 2nd ed.. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y.:
    [Google Scholar]
  45. Sears B. B., Lim P.-O., Holland N., Kirkpatrick B. C., Klomparens K. L. 1989; Isolation and characterization of DNA from a mycoplasmalike organism. Mol. Plant-Microbe Interact. 2:175–180
    [Google Scholar]
  46. Tabor S., Richardson C. C. 1989; Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase. Proc. Natl. Acad. Sci. USA 86:4076–4080
    [Google Scholar]
  47. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
    [Google Scholar]
  48. Williamson D. L., Tully J. G., Whitcomb R. F. 1989; The genus Spiroplasma. 71–111 Whitcomb R. F., Tully J. G. The mycoplasmas, vol. 5. Spiroplasmas, acholeplasmas, and mycoplasmas of plants and arthropods Academic Press; New York:
    [Google Scholar]
  49. Woese C. R., Maniloff J., Zablen L. B. 1980; Phylogenetic analysis of the mycoplasmas. Proc. Natl. Acad. Sci. USA 77:494–498
    [Google Scholar]
  50. Xia Z.-S., Kasahara H., Namba S., Yamashita S., Doi Y. 1987; Purification of mulberry dwarf MLO and production of antiserum. Ann. Phytopathol. Soc. Jpn. 53:122
    [Google Scholar]
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