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Volume 43, Issue 3

Isolation and Characterization of sp. nov. and DNA Reassociation Study of the Genus Free

Abstract

Abstract

Six strains of coccoid, halophilic methanogens were isolated from various salinaria and natural hypersaline environments. These isolates (strains FDF-1 [T = type strain], FDF-2, SF-2, Ret-1, SD-1, and Cas-1) grew on media containing methanol and mono-, di-, and trimethylamines as catabolic substrates, but not on media containing dimethyl sulfide, methane thiol, H, formate, or acetate; when cells were provided with H in addition to methanol or trimethylamine, they grew on the medium containing a methyl substrate but did not catabolize H. All of the strains were capable of growth in mineral medium to which trimethylamine was added as a catabolic substrate, although some strains were greatly stimulated by biotin or p-aminobenzoate. DNA reassociation and denaturing electrophoresis of whole-cell proteins indicated that strains FDF-1, FDF-2, SF-2, and Ret-1, together with previously described strains SF-1, Z-7302, Z-7401, Z-7404, and Z-7405, belong to a new taxon named sp. nov; FDF-1 (= OCM 59) is the type strain. These strains grew fastest at temperatures near 40°C and, in medium containing 0.5 to 2.5 M NaCl, at pH values near 7. The two new strains excluded from the species on the basis of the results of phylogenetic tests, strains Cas-1 and SD-1, also differed from in some minor physiological characteristics. Strain Cas-1 was less halophilic (fastest growth occurred in the presence of 0.5 to 1 M NaCl), and strain SD-1 was slightly alkaliphilic (fastest growth occurred at pH 7.8). The DNA reassociation study also showed that SLP exhibited 52% sequence similarity with Z-7982, supporting the classification of these organisms as separate but closely related species.

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Isolation and Characterization of sp. nov. and DNA Reassociation Study of the Genus
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1993-07-01
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Isolation and Characterization of Methanohalophilus portucalensis sp. nov. and DNA Reassociation Study of the Genus Methanohalophilus
Int J Syst Evol Microbiol 43, 430 (1993); https://doi.org/10.1099/00207713-43-3-430
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